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Antitumor effect of nuclear factor-κB decoy transfer by mannose-modified bubble lipoplex into macrophages in mouse malignant ascites.

Kono Y, Kawakami S, Higuchi Y, Maruyama K, Yamashita F, Hashida M - Cancer Sci. (2014)

Bottom Line: Patients with malignant ascites (MAs) display several symptoms, such as dyspnea, nausea, pain, and abdominal tenderness, resulting in a significant reduction in their quality of life.In contrast, mRNA levels of Th1 cytokines (IL-12, tumor necrosis factor-α, and IL-6) were increased significantly.Moreover, the expression level of vascular endothelial growth factor in ascites was suppressed significantly, and peritoneal angiogenesis showed a reduction.

View Article: PubMed Central - PubMed

Affiliation: Department of Drug Delivery Research, Graduate School of Pharmaceutical Sciences, Kyoto University, Kyoto, Japan.

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Effect of in vivo nuclear factor-κB (NF-κB) decoy transfection by ultrasound (US)-responsive and mannose-unmodified liposome/NF-κB decoy complexes (Bare-PEG bubble lipoplexes) or ultrasound (US)-responsive and mannose-modified liposome/NF-κB decoy complexes (Man-PEG bubble lipoplexes) (10 μg NF-κB decoy) with or without US exposure on cytokine mRNA expression in tumor-associated macrophages. mRNA levels of interleukin-10 (IL-10) (a), IL-12 (b), tumor necrosis factor-α (TNF-α) (c), and IL-6 (d) in tumor-associated macrophages collected at 24 h post-transfection. Data are represented as the mean + SD (n = 4). *P < 0.05, **P < 0.01 compared with non-treatment (N.T.).
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fig03: Effect of in vivo nuclear factor-κB (NF-κB) decoy transfection by ultrasound (US)-responsive and mannose-unmodified liposome/NF-κB decoy complexes (Bare-PEG bubble lipoplexes) or ultrasound (US)-responsive and mannose-modified liposome/NF-κB decoy complexes (Man-PEG bubble lipoplexes) (10 μg NF-κB decoy) with or without US exposure on cytokine mRNA expression in tumor-associated macrophages. mRNA levels of interleukin-10 (IL-10) (a), IL-12 (b), tumor necrosis factor-α (TNF-α) (c), and IL-6 (d) in tumor-associated macrophages collected at 24 h post-transfection. Data are represented as the mean + SD (n = 4). *P < 0.05, **P < 0.01 compared with non-treatment (N.T.).

Mentions: Because the phenotype of macrophages is generally classified according to the profile of cytokine expression,(15,16) we assessed the expression levels of cytokine mRNA in TAMs transfected with the NF-κB decoy. Expression of IL-10 mRNA in TAMs transfected with the NF-κB decoy by Man-PEG bubble lipoplexes and US exposure was the lowest among all groups (Fig. 3a). In contrast, mRNA expression levels of IL-12, TNF-α, and IL-6 in TAMs transfected with the NF-κB decoy by Man-PEG bubble lipoplexes and US exposure were significantly higher compared with those in other groups (Fig. 3b–d).


Antitumor effect of nuclear factor-κB decoy transfer by mannose-modified bubble lipoplex into macrophages in mouse malignant ascites.

Kono Y, Kawakami S, Higuchi Y, Maruyama K, Yamashita F, Hashida M - Cancer Sci. (2014)

Effect of in vivo nuclear factor-κB (NF-κB) decoy transfection by ultrasound (US)-responsive and mannose-unmodified liposome/NF-κB decoy complexes (Bare-PEG bubble lipoplexes) or ultrasound (US)-responsive and mannose-modified liposome/NF-κB decoy complexes (Man-PEG bubble lipoplexes) (10 μg NF-κB decoy) with or without US exposure on cytokine mRNA expression in tumor-associated macrophages. mRNA levels of interleukin-10 (IL-10) (a), IL-12 (b), tumor necrosis factor-α (TNF-α) (c), and IL-6 (d) in tumor-associated macrophages collected at 24 h post-transfection. Data are represented as the mean + SD (n = 4). *P < 0.05, **P < 0.01 compared with non-treatment (N.T.).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4317846&req=5

fig03: Effect of in vivo nuclear factor-κB (NF-κB) decoy transfection by ultrasound (US)-responsive and mannose-unmodified liposome/NF-κB decoy complexes (Bare-PEG bubble lipoplexes) or ultrasound (US)-responsive and mannose-modified liposome/NF-κB decoy complexes (Man-PEG bubble lipoplexes) (10 μg NF-κB decoy) with or without US exposure on cytokine mRNA expression in tumor-associated macrophages. mRNA levels of interleukin-10 (IL-10) (a), IL-12 (b), tumor necrosis factor-α (TNF-α) (c), and IL-6 (d) in tumor-associated macrophages collected at 24 h post-transfection. Data are represented as the mean + SD (n = 4). *P < 0.05, **P < 0.01 compared with non-treatment (N.T.).
Mentions: Because the phenotype of macrophages is generally classified according to the profile of cytokine expression,(15,16) we assessed the expression levels of cytokine mRNA in TAMs transfected with the NF-κB decoy. Expression of IL-10 mRNA in TAMs transfected with the NF-κB decoy by Man-PEG bubble lipoplexes and US exposure was the lowest among all groups (Fig. 3a). In contrast, mRNA expression levels of IL-12, TNF-α, and IL-6 in TAMs transfected with the NF-κB decoy by Man-PEG bubble lipoplexes and US exposure were significantly higher compared with those in other groups (Fig. 3b–d).

Bottom Line: Patients with malignant ascites (MAs) display several symptoms, such as dyspnea, nausea, pain, and abdominal tenderness, resulting in a significant reduction in their quality of life.In contrast, mRNA levels of Th1 cytokines (IL-12, tumor necrosis factor-α, and IL-6) were increased significantly.Moreover, the expression level of vascular endothelial growth factor in ascites was suppressed significantly, and peritoneal angiogenesis showed a reduction.

View Article: PubMed Central - PubMed

Affiliation: Department of Drug Delivery Research, Graduate School of Pharmaceutical Sciences, Kyoto University, Kyoto, Japan.

Show MeSH
Related in: MedlinePlus