Antitumor effect of nuclear factor-κB decoy transfer by mannose-modified bubble lipoplex into macrophages in mouse malignant ascites.
Bottom Line: Patients with malignant ascites (MAs) display several symptoms, such as dyspnea, nausea, pain, and abdominal tenderness, resulting in a significant reduction in their quality of life.In contrast, mRNA levels of Th1 cytokines (IL-12, tumor necrosis factor-α, and IL-6) were increased significantly.Moreover, the expression level of vascular endothelial growth factor in ascites was suppressed significantly, and peritoneal angiogenesis showed a reduction.
Affiliation: Department of Drug Delivery Research, Graduate School of Pharmaceutical Sciences, Kyoto University, Kyoto, Japan.Show MeSH
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Mentions: We carried out in vivo TAM-targeted NF-κB decoy transfer by Man-PEG bubble lipoplexes constructed with a FAM-labeled NF-κB decoy combined with US exposure. The amount of FAM-labeled NF-κB decoy introduced into TAMs by Man-PEG bubble lipoplexes and US exposure was eightfold higher than that by Man-PEG bubble lipoplexes without US exposure (Fig. 1). Moreover, this level of FAM-labeled NF-κB decoy transfection by Man-PEG bubble lipoplexes and US exposure was much higher than that by Bare-PEG bubble lipoplexes and US exposure. Similarly, intranuclear p50 and p65 levels in TAMs were significantly decreased by NF-κB decoy transfer using Man-PEG bubble lipoplexes and US exposure (Fig. 2).
Affiliation: Department of Drug Delivery Research, Graduate School of Pharmaceutical Sciences, Kyoto University, Kyoto, Japan.