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Antitumor effect of nuclear factor-κB decoy transfer by mannose-modified bubble lipoplex into macrophages in mouse malignant ascites.

Kono Y, Kawakami S, Higuchi Y, Maruyama K, Yamashita F, Hashida M - Cancer Sci. (2014)

Bottom Line: Patients with malignant ascites (MAs) display several symptoms, such as dyspnea, nausea, pain, and abdominal tenderness, resulting in a significant reduction in their quality of life.In contrast, mRNA levels of Th1 cytokines (IL-12, tumor necrosis factor-α, and IL-6) were increased significantly.Moreover, the expression level of vascular endothelial growth factor in ascites was suppressed significantly, and peritoneal angiogenesis showed a reduction.

View Article: PubMed Central - PubMed

Affiliation: Department of Drug Delivery Research, Graduate School of Pharmaceutical Sciences, Kyoto University, Kyoto, Japan.

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In vivo transfection efficiency of the nuclear factor-κB (NF-κB) decoy into tumor-associated macrophages by ultrasound (US)-responsive and mannose-unmodified liposome/NF-κB decoy com-plexes (Bare-PEG bubble lipoplexes) and ultrasound (US)-responsive and mannose-modified liposome/NF-κB decoy complexes (Man-PEG bubble lipoplexes) (10 μg NF-κB decoy) with (+) or without (−) US exposure. Fluorescent intensity of the FAM-labeled NF-κB decoy in tumor-associated macrophages at 1 h after addition of bubble lipoplexes. **P < 0.01.
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fig01: In vivo transfection efficiency of the nuclear factor-κB (NF-κB) decoy into tumor-associated macrophages by ultrasound (US)-responsive and mannose-unmodified liposome/NF-κB decoy com-plexes (Bare-PEG bubble lipoplexes) and ultrasound (US)-responsive and mannose-modified liposome/NF-κB decoy complexes (Man-PEG bubble lipoplexes) (10 μg NF-κB decoy) with (+) or without (−) US exposure. Fluorescent intensity of the FAM-labeled NF-κB decoy in tumor-associated macrophages at 1 h after addition of bubble lipoplexes. **P < 0.01.

Mentions: We carried out in vivo TAM-targeted NF-κB decoy transfer by Man-PEG bubble lipoplexes constructed with a FAM-labeled NF-κB decoy combined with US exposure. The amount of FAM-labeled NF-κB decoy introduced into TAMs by Man-PEG bubble lipoplexes and US exposure was eightfold higher than that by Man-PEG bubble lipoplexes without US exposure (Fig. 1). Moreover, this level of FAM-labeled NF-κB decoy transfection by Man-PEG bubble lipoplexes and US exposure was much higher than that by Bare-PEG bubble lipoplexes and US exposure. Similarly, intranuclear p50 and p65 levels in TAMs were significantly decreased by NF-κB decoy transfer using Man-PEG bubble lipoplexes and US exposure (Fig. 2).


Antitumor effect of nuclear factor-κB decoy transfer by mannose-modified bubble lipoplex into macrophages in mouse malignant ascites.

Kono Y, Kawakami S, Higuchi Y, Maruyama K, Yamashita F, Hashida M - Cancer Sci. (2014)

In vivo transfection efficiency of the nuclear factor-κB (NF-κB) decoy into tumor-associated macrophages by ultrasound (US)-responsive and mannose-unmodified liposome/NF-κB decoy com-plexes (Bare-PEG bubble lipoplexes) and ultrasound (US)-responsive and mannose-modified liposome/NF-κB decoy complexes (Man-PEG bubble lipoplexes) (10 μg NF-κB decoy) with (+) or without (−) US exposure. Fluorescent intensity of the FAM-labeled NF-κB decoy in tumor-associated macrophages at 1 h after addition of bubble lipoplexes. **P < 0.01.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4317846&req=5

fig01: In vivo transfection efficiency of the nuclear factor-κB (NF-κB) decoy into tumor-associated macrophages by ultrasound (US)-responsive and mannose-unmodified liposome/NF-κB decoy com-plexes (Bare-PEG bubble lipoplexes) and ultrasound (US)-responsive and mannose-modified liposome/NF-κB decoy complexes (Man-PEG bubble lipoplexes) (10 μg NF-κB decoy) with (+) or without (−) US exposure. Fluorescent intensity of the FAM-labeled NF-κB decoy in tumor-associated macrophages at 1 h after addition of bubble lipoplexes. **P < 0.01.
Mentions: We carried out in vivo TAM-targeted NF-κB decoy transfer by Man-PEG bubble lipoplexes constructed with a FAM-labeled NF-κB decoy combined with US exposure. The amount of FAM-labeled NF-κB decoy introduced into TAMs by Man-PEG bubble lipoplexes and US exposure was eightfold higher than that by Man-PEG bubble lipoplexes without US exposure (Fig. 1). Moreover, this level of FAM-labeled NF-κB decoy transfection by Man-PEG bubble lipoplexes and US exposure was much higher than that by Bare-PEG bubble lipoplexes and US exposure. Similarly, intranuclear p50 and p65 levels in TAMs were significantly decreased by NF-κB decoy transfer using Man-PEG bubble lipoplexes and US exposure (Fig. 2).

Bottom Line: Patients with malignant ascites (MAs) display several symptoms, such as dyspnea, nausea, pain, and abdominal tenderness, resulting in a significant reduction in their quality of life.In contrast, mRNA levels of Th1 cytokines (IL-12, tumor necrosis factor-α, and IL-6) were increased significantly.Moreover, the expression level of vascular endothelial growth factor in ascites was suppressed significantly, and peritoneal angiogenesis showed a reduction.

View Article: PubMed Central - PubMed

Affiliation: Department of Drug Delivery Research, Graduate School of Pharmaceutical Sciences, Kyoto University, Kyoto, Japan.

Show MeSH
Related in: MedlinePlus