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Duodenal follicular lymphoma: comprehensive gene expression analysis with insights into pathogenesis.

Takata K, Tanino M, Ennishi D, Tari A, Sato Y, Okada H, Maeda Y, Goto N, Araki H, Harada M, Ando M, Iwamuro M, Tanimoto M, Yamamoto K, Gascoyne RD, Yoshino T - Cancer Sci. (2014)

Bottom Line: In contrast, protocadherin gamma subfamily genes were upregulated in DFL and NFL.Double immunofluorescence studies revealed that CCL20 and CCR6 were co-expressed in both DFL and MALT.We hypothesize that increased expression of CCL20 and MAdCAM-1 and co-expression of CCL20 and CCR6 may play an important role in tumorigenesis.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Okayama, Japan.

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Validation of microarray data and double immunohistochemical staining for CCL20 and CCR6. (a–i) Immunohistochemical results for PCDHGA3, PCDHGA8 and PCDHGB4. Duodenal follicular lymphoma (DFL) and nodal follicular lymphoma (NFL) were positive for PCDHGA3 (a) and (c), PCDHGA8 (d) and (f), and PCDHGB4 (g) and (i), while MALT was negative for all three markers (b), (e) and (h). (j) Double immunohistochemical staining for CCL20 and CCR6 in DFL. Tumor cells co-expressed CCL20 and CCR6.
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fig05: Validation of microarray data and double immunohistochemical staining for CCL20 and CCR6. (a–i) Immunohistochemical results for PCDHGA3, PCDHGA8 and PCDHGB4. Duodenal follicular lymphoma (DFL) and nodal follicular lymphoma (NFL) were positive for PCDHGA3 (a) and (c), PCDHGA8 (d) and (f), and PCDHGB4 (g) and (i), while MALT was negative for all three markers (b), (e) and (h). (j) Double immunohistochemical staining for CCL20 and CCR6 in DFL. Tumor cells co-expressed CCL20 and CCR6.

Mentions: Immunohistochemical studies revealed that CCL20 protein expression was significantly higher in DFL and MALT samples than in NFL samples (P < 0.001). CCL20 was positive in 97% (31/32) of DFL samples and 89% (17/19) of MALT samples, but only 4% (1/27) of NFL samples (Fig.4c–e). MAdCAM-1 expression, present in vascular endothelial cells, was more frequently expressed in DFL (28/32, 88%) and MALT (18/19, 95%) samples than in NFL samples (5/27, 19%; Fig.4f–h). This difference was also statistically significant (P < 0.001). In contrast, the expression of PCDHGA8, A3 and B4 was significantly higher in DFL and NFL than in MALT (Fig.5a–i). A summary of the immunohistochemical results is shown in Table1.


Duodenal follicular lymphoma: comprehensive gene expression analysis with insights into pathogenesis.

Takata K, Tanino M, Ennishi D, Tari A, Sato Y, Okada H, Maeda Y, Goto N, Araki H, Harada M, Ando M, Iwamuro M, Tanimoto M, Yamamoto K, Gascoyne RD, Yoshino T - Cancer Sci. (2014)

Validation of microarray data and double immunohistochemical staining for CCL20 and CCR6. (a–i) Immunohistochemical results for PCDHGA3, PCDHGA8 and PCDHGB4. Duodenal follicular lymphoma (DFL) and nodal follicular lymphoma (NFL) were positive for PCDHGA3 (a) and (c), PCDHGA8 (d) and (f), and PCDHGB4 (g) and (i), while MALT was negative for all three markers (b), (e) and (h). (j) Double immunohistochemical staining for CCL20 and CCR6 in DFL. Tumor cells co-expressed CCL20 and CCR6.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4317842&req=5

fig05: Validation of microarray data and double immunohistochemical staining for CCL20 and CCR6. (a–i) Immunohistochemical results for PCDHGA3, PCDHGA8 and PCDHGB4. Duodenal follicular lymphoma (DFL) and nodal follicular lymphoma (NFL) were positive for PCDHGA3 (a) and (c), PCDHGA8 (d) and (f), and PCDHGB4 (g) and (i), while MALT was negative for all three markers (b), (e) and (h). (j) Double immunohistochemical staining for CCL20 and CCR6 in DFL. Tumor cells co-expressed CCL20 and CCR6.
Mentions: Immunohistochemical studies revealed that CCL20 protein expression was significantly higher in DFL and MALT samples than in NFL samples (P < 0.001). CCL20 was positive in 97% (31/32) of DFL samples and 89% (17/19) of MALT samples, but only 4% (1/27) of NFL samples (Fig.4c–e). MAdCAM-1 expression, present in vascular endothelial cells, was more frequently expressed in DFL (28/32, 88%) and MALT (18/19, 95%) samples than in NFL samples (5/27, 19%; Fig.4f–h). This difference was also statistically significant (P < 0.001). In contrast, the expression of PCDHGA8, A3 and B4 was significantly higher in DFL and NFL than in MALT (Fig.5a–i). A summary of the immunohistochemical results is shown in Table1.

Bottom Line: In contrast, protocadherin gamma subfamily genes were upregulated in DFL and NFL.Double immunofluorescence studies revealed that CCL20 and CCR6 were co-expressed in both DFL and MALT.We hypothesize that increased expression of CCL20 and MAdCAM-1 and co-expression of CCL20 and CCR6 may play an important role in tumorigenesis.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Okayama, Japan.

Show MeSH
Related in: MedlinePlus