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Duodenal follicular lymphoma: comprehensive gene expression analysis with insights into pathogenesis.

Takata K, Tanino M, Ennishi D, Tari A, Sato Y, Okada H, Maeda Y, Goto N, Araki H, Harada M, Ando M, Iwamuro M, Tanimoto M, Yamamoto K, Gascoyne RD, Yoshino T - Cancer Sci. (2014)

Bottom Line: In contrast, protocadherin gamma subfamily genes were upregulated in DFL and NFL.Double immunofluorescence studies revealed that CCL20 and CCR6 were co-expressed in both DFL and MALT.We hypothesize that increased expression of CCL20 and MAdCAM-1 and co-expression of CCL20 and CCR6 may play an important role in tumorigenesis.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Okayama, Japan.

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Related in: MedlinePlus

Validation of microarray data by quantitative RT-PCR and immunohistochemical studies. (a, b) Quantitative RT-PCR for CCL20 and MAdCAM-1, respectively. duodenal follicular lymphoma (DFL) and MALT showed higher expression levels of CCL20 and MAdCAM-1 than nodal follicular lymphoma (NFL) (P < 0.001). The coefficient of correlation with the microarray data was 0.925 for CCL20 and 0.7934 for MAdCAM-1. (c–k) Immunohistochemical results for CCL20, MAdCAM-1 and CCR6 in DFL, MALT and NFL. DFL and MALT were positive for CCL20 (c) and (d), while NFL was negative for CCL20 (e). Vascular endothelial cells were highly expressed in DFL and MALT (f) and (g), but not in NFL (h). DFL (i), MALT (j) and NFL (k) were positive for CCR6.
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fig04: Validation of microarray data by quantitative RT-PCR and immunohistochemical studies. (a, b) Quantitative RT-PCR for CCL20 and MAdCAM-1, respectively. duodenal follicular lymphoma (DFL) and MALT showed higher expression levels of CCL20 and MAdCAM-1 than nodal follicular lymphoma (NFL) (P < 0.001). The coefficient of correlation with the microarray data was 0.925 for CCL20 and 0.7934 for MAdCAM-1. (c–k) Immunohistochemical results for CCL20, MAdCAM-1 and CCR6 in DFL, MALT and NFL. DFL and MALT were positive for CCL20 (c) and (d), while NFL was negative for CCL20 (e). Vascular endothelial cells were highly expressed in DFL and MALT (f) and (g), but not in NFL (h). DFL (i), MALT (j) and NFL (k) were positive for CCR6.

Mentions: The set of genes selected for validation by quantitative RT-PCR and immunohistochemistry included DEG, CCL20 (that was common in DFL and MALT, with opposite expression in NFL) and MAdCAM-1 (common in DFL and NFL, with opposite expression in MALT). Quantitative RT-PCR analysis showed significantly higher expression of CCL20 and MAdCAM-1 in DFL and MALT versus NFL. Coefficients of correlation with the microarray were 0.9245 for CCL20 and 0.7934 for MAdCAM-1 (Fig.4a,b). MAdCAM-1 was slightly upregulated in 5 of 18 NFL samples. These samples were all abdominal or mesenteric lymph nodes.


Duodenal follicular lymphoma: comprehensive gene expression analysis with insights into pathogenesis.

Takata K, Tanino M, Ennishi D, Tari A, Sato Y, Okada H, Maeda Y, Goto N, Araki H, Harada M, Ando M, Iwamuro M, Tanimoto M, Yamamoto K, Gascoyne RD, Yoshino T - Cancer Sci. (2014)

Validation of microarray data by quantitative RT-PCR and immunohistochemical studies. (a, b) Quantitative RT-PCR for CCL20 and MAdCAM-1, respectively. duodenal follicular lymphoma (DFL) and MALT showed higher expression levels of CCL20 and MAdCAM-1 than nodal follicular lymphoma (NFL) (P < 0.001). The coefficient of correlation with the microarray data was 0.925 for CCL20 and 0.7934 for MAdCAM-1. (c–k) Immunohistochemical results for CCL20, MAdCAM-1 and CCR6 in DFL, MALT and NFL. DFL and MALT were positive for CCL20 (c) and (d), while NFL was negative for CCL20 (e). Vascular endothelial cells were highly expressed in DFL and MALT (f) and (g), but not in NFL (h). DFL (i), MALT (j) and NFL (k) were positive for CCR6.
© Copyright Policy - open-access
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4317842&req=5

fig04: Validation of microarray data by quantitative RT-PCR and immunohistochemical studies. (a, b) Quantitative RT-PCR for CCL20 and MAdCAM-1, respectively. duodenal follicular lymphoma (DFL) and MALT showed higher expression levels of CCL20 and MAdCAM-1 than nodal follicular lymphoma (NFL) (P < 0.001). The coefficient of correlation with the microarray data was 0.925 for CCL20 and 0.7934 for MAdCAM-1. (c–k) Immunohistochemical results for CCL20, MAdCAM-1 and CCR6 in DFL, MALT and NFL. DFL and MALT were positive for CCL20 (c) and (d), while NFL was negative for CCL20 (e). Vascular endothelial cells were highly expressed in DFL and MALT (f) and (g), but not in NFL (h). DFL (i), MALT (j) and NFL (k) were positive for CCR6.
Mentions: The set of genes selected for validation by quantitative RT-PCR and immunohistochemistry included DEG, CCL20 (that was common in DFL and MALT, with opposite expression in NFL) and MAdCAM-1 (common in DFL and NFL, with opposite expression in MALT). Quantitative RT-PCR analysis showed significantly higher expression of CCL20 and MAdCAM-1 in DFL and MALT versus NFL. Coefficients of correlation with the microarray were 0.9245 for CCL20 and 0.7934 for MAdCAM-1 (Fig.4a,b). MAdCAM-1 was slightly upregulated in 5 of 18 NFL samples. These samples were all abdominal or mesenteric lymph nodes.

Bottom Line: In contrast, protocadherin gamma subfamily genes were upregulated in DFL and NFL.Double immunofluorescence studies revealed that CCL20 and CCR6 were co-expressed in both DFL and MALT.We hypothesize that increased expression of CCL20 and MAdCAM-1 and co-expression of CCL20 and CCR6 may play an important role in tumorigenesis.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Okayama, Japan.

Show MeSH
Related in: MedlinePlus