Duodenal follicular lymphoma: comprehensive gene expression analysis with insights into pathogenesis.
Bottom Line: In contrast, protocadherin gamma subfamily genes were upregulated in DFL and NFL.Double immunofluorescence studies revealed that CCL20 and CCR6 were co-expressed in both DFL and MALT.We hypothesize that increased expression of CCL20 and MAdCAM-1 and co-expression of CCL20 and CCR6 may play an important role in tumorigenesis.
Affiliation: Department of Pathology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Okayama, Japan.Show MeSH
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Mentions: The set of genes selected for validation by quantitative RT-PCR and immunohistochemistry included DEG, CCL20 (that was common in DFL and MALT, with opposite expression in NFL) and MAdCAM-1 (common in DFL and NFL, with opposite expression in MALT). Quantitative RT-PCR analysis showed significantly higher expression of CCL20 and MAdCAM-1 in DFL and MALT versus NFL. Coefficients of correlation with the microarray were 0.9245 for CCL20 and 0.7934 for MAdCAM-1 (Fig.4a,b). MAdCAM-1 was slightly upregulated in 5 of 18 NFL samples. These samples were all abdominal or mesenteric lymph nodes.
Affiliation: Department of Pathology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Okayama, Japan.