Limits...
Gene expression profiling of Epstein-Barr virus-positive diffuse large B-cell lymphoma of the elderly reveals alterations of characteristic oncogenetic pathways.

Kato H, Karube K, Yamamoto K, Takizawa J, Tsuzuki S, Yatabe Y, Kanda T, Katayama M, Ozawa Y, Ishitsuka K, Okamoto M, Kinoshita T, Ohshima K, Nakamura S, Morishima Y, Seto M - Cancer Sci. (2014)

Bottom Line: To confirm the results of the expression profiles, in vitro analysis was performed.The results of the present study suggest that activation of the JAK-STAT and NF-κB pathways was characteristic of EBV(+)DLBCL-E, which may reflect the nature of EBV-positive tumor cells.Targeting these pathways as therapies might improve clinical outcomes of EBV(+)DLBCL-E.

View Article: PubMed Central - PubMed

Affiliation: Division of Molecular Medicine, Aichi Cancer Center Research Institute, Nagoya, Aichi, Japan; Department of Hematology and Cell Therapy, Aichi Cancer Center Hospital, Nagoya, Aichi, Japan; Department of Cancer Genetics, Nagoya University Graduate School of Medicine, Nagoya, Aichi, Japan.

Show MeSH

Related in: MedlinePlus

Supervised analysis and clustering results for significant genes expressed in Epstein–Barr virus-positive diffuse large B-cell lymphoma of the elderly (EBV[+]DLBCL-E). Hierarchical clustering of the probes with an average expression level of more than 0.5 log fold-change in five cases of EBV(+)DLBCL-E and seven cases of EBV-negative DLBCL (EBV[−]DLBCL). Supervised analysis showed the distinct separation of EBV(+)DLBCL-E as a single cluster. ABC, activated B-cell; GCB, germinal center B-cell.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4317839&req=5

fig01: Supervised analysis and clustering results for significant genes expressed in Epstein–Barr virus-positive diffuse large B-cell lymphoma of the elderly (EBV[+]DLBCL-E). Hierarchical clustering of the probes with an average expression level of more than 0.5 log fold-change in five cases of EBV(+)DLBCL-E and seven cases of EBV-negative DLBCL (EBV[−]DLBCL). Supervised analysis showed the distinct separation of EBV(+)DLBCL-E as a single cluster. ABC, activated B-cell; GCB, germinal center B-cell.

Mentions: After normalization of the raw data, 36 693 probes were used for further analyses. Among these probes, a total of 1432 showed a P > 0.05 with a false discovery rate (FDR) <25%. The number of probes showing high expression in EBV(+)DLBCL-E and EBV(−)DLBCL was 545 (including 474 genes) and 887 (including 767 genes), respectively. First we selected the differentially expressed probes with an average expression level of more than 0.5 log fold-change (268 probes in EBV[+]DLBCL-E and 275 probes in EBV[−]DLBCL), which could separate EBV(+)DLBCL-E cases as a single cluster (Fig.1).


Gene expression profiling of Epstein-Barr virus-positive diffuse large B-cell lymphoma of the elderly reveals alterations of characteristic oncogenetic pathways.

Kato H, Karube K, Yamamoto K, Takizawa J, Tsuzuki S, Yatabe Y, Kanda T, Katayama M, Ozawa Y, Ishitsuka K, Okamoto M, Kinoshita T, Ohshima K, Nakamura S, Morishima Y, Seto M - Cancer Sci. (2014)

Supervised analysis and clustering results for significant genes expressed in Epstein–Barr virus-positive diffuse large B-cell lymphoma of the elderly (EBV[+]DLBCL-E). Hierarchical clustering of the probes with an average expression level of more than 0.5 log fold-change in five cases of EBV(+)DLBCL-E and seven cases of EBV-negative DLBCL (EBV[−]DLBCL). Supervised analysis showed the distinct separation of EBV(+)DLBCL-E as a single cluster. ABC, activated B-cell; GCB, germinal center B-cell.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4317839&req=5

fig01: Supervised analysis and clustering results for significant genes expressed in Epstein–Barr virus-positive diffuse large B-cell lymphoma of the elderly (EBV[+]DLBCL-E). Hierarchical clustering of the probes with an average expression level of more than 0.5 log fold-change in five cases of EBV(+)DLBCL-E and seven cases of EBV-negative DLBCL (EBV[−]DLBCL). Supervised analysis showed the distinct separation of EBV(+)DLBCL-E as a single cluster. ABC, activated B-cell; GCB, germinal center B-cell.
Mentions: After normalization of the raw data, 36 693 probes were used for further analyses. Among these probes, a total of 1432 showed a P > 0.05 with a false discovery rate (FDR) <25%. The number of probes showing high expression in EBV(+)DLBCL-E and EBV(−)DLBCL was 545 (including 474 genes) and 887 (including 767 genes), respectively. First we selected the differentially expressed probes with an average expression level of more than 0.5 log fold-change (268 probes in EBV[+]DLBCL-E and 275 probes in EBV[−]DLBCL), which could separate EBV(+)DLBCL-E cases as a single cluster (Fig.1).

Bottom Line: To confirm the results of the expression profiles, in vitro analysis was performed.The results of the present study suggest that activation of the JAK-STAT and NF-κB pathways was characteristic of EBV(+)DLBCL-E, which may reflect the nature of EBV-positive tumor cells.Targeting these pathways as therapies might improve clinical outcomes of EBV(+)DLBCL-E.

View Article: PubMed Central - PubMed

Affiliation: Division of Molecular Medicine, Aichi Cancer Center Research Institute, Nagoya, Aichi, Japan; Department of Hematology and Cell Therapy, Aichi Cancer Center Hospital, Nagoya, Aichi, Japan; Department of Cancer Genetics, Nagoya University Graduate School of Medicine, Nagoya, Aichi, Japan.

Show MeSH
Related in: MedlinePlus