Antitumor effect of fibrin glue containing temozolomide against malignant glioma.
Bottom Line: Our high-power liquid chromatography studies indicated that FG containing TMZ (TMZ-FG) manifested a sustained drug release potential.In both phases, TMZ-FG failed to severely damage normal brain tissue.TMZ-FG may represent a safe new drug delivery system with sustained drug release potential to treat malignant glioma.
Affiliation: Department of Neurosurgery, Kumamoto University Graduate School of Medical Science, Honjo, Chuo-ku, Kumamoto, Japan.Show MeSH
Related in: MedlinePlus
Mentions: To investigate the effect of FGS containing 1 mM TMZ and of FGS alone on the brain tissue of normal ICR mice in the acute and chronic phase, we stained brain tissue samples with H&E, GFAP, COX-2 and Nissl to investigate the inflammatory responses of astrocytes, the infiltration of other cells and demyelination. In the acute phase, GFAP and COX-2 staining around the FGS and TMZ-FGS was slightly stronger than in the controls; H&E and Nissl staining was not markedly different from the controls (Fig.6a). In the chronic phase we observed no staining difference (Fig.6b). Treatment with FGS containing TMZ did not result in severe edema, demyelination or inflammation of the normal brain in both phases after FGS placement. All FGS had retained their original shape at day 14. None of the mice manifested weight loss, infections or delayed healing and none died during the observation period (data not shown). Based on these findings we suggest that FGS containing TMZ is safe for normal brain tissue.
Affiliation: Department of Neurosurgery, Kumamoto University Graduate School of Medical Science, Honjo, Chuo-ku, Kumamoto, Japan.