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Antitumor effect of fibrin glue containing temozolomide against malignant glioma.

Anai S, Hide T, Takezaki T, Kuroda J, Shinojima N, Makino K, Nakamura H, Yano S, Kuratsu J - Cancer Sci. (2014)

Bottom Line: Our high-power liquid chromatography studies indicated that FG containing TMZ (TMZ-FG) manifested a sustained drug release potential.In both phases, TMZ-FG failed to severely damage normal brain tissue.TMZ-FG may represent a safe new drug delivery system with sustained drug release potential to treat malignant glioma.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurosurgery, Kumamoto University Graduate School of Medical Science, Honjo, Chuo-ku, Kumamoto, Japan.

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Related in: MedlinePlus

Western blot analysis of subcutaneous tumors. (a) Western blots of cleaved caspase 3, LC3-I, LC3-II, p16 and β-tubulin in subcutaneous tumors treated for 7 days with fibrin glue sheets (FGS) alone (control) or temozolomide (TMZ-FGS) (n = 3). (b) Graphs of the intensity of the bands. Analysis was with the unpaired two-tailed t-test. A P < 0.05 was considered statistically significant. *P < 0.05. **P < 0.001.
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fig05: Western blot analysis of subcutaneous tumors. (a) Western blots of cleaved caspase 3, LC3-I, LC3-II, p16 and β-tubulin in subcutaneous tumors treated for 7 days with fibrin glue sheets (FGS) alone (control) or temozolomide (TMZ-FGS) (n = 3). (b) Graphs of the intensity of the bands. Analysis was with the unpaired two-tailed t-test. A P < 0.05 was considered statistically significant. *P < 0.05. **P < 0.001.

Mentions: Western blot analysis showed that the expression of cleaved caspase 3, LC3 and p16 was significantly higher in mice treated with TMZ-FGS than in the controls (Fig.5). An increase in the LC3-II/LC3-I level is correlated with an increase in autophagy.


Antitumor effect of fibrin glue containing temozolomide against malignant glioma.

Anai S, Hide T, Takezaki T, Kuroda J, Shinojima N, Makino K, Nakamura H, Yano S, Kuratsu J - Cancer Sci. (2014)

Western blot analysis of subcutaneous tumors. (a) Western blots of cleaved caspase 3, LC3-I, LC3-II, p16 and β-tubulin in subcutaneous tumors treated for 7 days with fibrin glue sheets (FGS) alone (control) or temozolomide (TMZ-FGS) (n = 3). (b) Graphs of the intensity of the bands. Analysis was with the unpaired two-tailed t-test. A P < 0.05 was considered statistically significant. *P < 0.05. **P < 0.001.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4317836&req=5

fig05: Western blot analysis of subcutaneous tumors. (a) Western blots of cleaved caspase 3, LC3-I, LC3-II, p16 and β-tubulin in subcutaneous tumors treated for 7 days with fibrin glue sheets (FGS) alone (control) or temozolomide (TMZ-FGS) (n = 3). (b) Graphs of the intensity of the bands. Analysis was with the unpaired two-tailed t-test. A P < 0.05 was considered statistically significant. *P < 0.05. **P < 0.001.
Mentions: Western blot analysis showed that the expression of cleaved caspase 3, LC3 and p16 was significantly higher in mice treated with TMZ-FGS than in the controls (Fig.5). An increase in the LC3-II/LC3-I level is correlated with an increase in autophagy.

Bottom Line: Our high-power liquid chromatography studies indicated that FG containing TMZ (TMZ-FG) manifested a sustained drug release potential.In both phases, TMZ-FG failed to severely damage normal brain tissue.TMZ-FG may represent a safe new drug delivery system with sustained drug release potential to treat malignant glioma.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurosurgery, Kumamoto University Graduate School of Medical Science, Honjo, Chuo-ku, Kumamoto, Japan.

Show MeSH
Related in: MedlinePlus