Antitumor effect of fibrin glue containing temozolomide against malignant glioma.
Bottom Line: Our high-power liquid chromatography studies indicated that FG containing TMZ (TMZ-FG) manifested a sustained drug release potential.In both phases, TMZ-FG failed to severely damage normal brain tissue.TMZ-FG may represent a safe new drug delivery system with sustained drug release potential to treat malignant glioma.
Affiliation: Department of Neurosurgery, Kumamoto University Graduate School of Medical Science, Honjo, Chuo-ku, Kumamoto, Japan.Show MeSH
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Mentions: We used Ki-67, cleaved caspase3, LC3 and p16 as markers for proliferation, apoptosis, autophagy and senescence. At high-power magnification, we counted immunohistochemically positive cells among the tumor cells around the FGS in 10 fields. The Ki-67-positive ratio was lower in all TMZ-treated mice than in the controls. In contrast, the ratio of cleaved caspase 3, LC3 and p16 positivity was higher in TMZ-treated mice than in the controls (Fig.4a,b; Table S2). Changes induced by TMZ were observed mainly on the tumor side covered with the TMZ-FGS. Such findings were not detected in mice treated with FGS only or with oral TMZ only (Fig.4c).
Affiliation: Department of Neurosurgery, Kumamoto University Graduate School of Medical Science, Honjo, Chuo-ku, Kumamoto, Japan.