Antitumor effect of fibrin glue containing temozolomide against malignant glioma.
Bottom Line: Our high-power liquid chromatography studies indicated that FG containing TMZ (TMZ-FG) manifested a sustained drug release potential.In both phases, TMZ-FG failed to severely damage normal brain tissue.TMZ-FG may represent a safe new drug delivery system with sustained drug release potential to treat malignant glioma.
Affiliation: Department of Neurosurgery, Kumamoto University Graduate School of Medical Science, Honjo, Chuo-ku, Kumamoto, Japan.Show MeSH
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Mentions: We performed HPLC three times per sample to assay the release of TMZ from FGS containing 0.5 mM or 1 mM TMZ. The TMZ peak of the calibration curve standards and samples appeared at approximately 2.19 min (Fig.2a,b). The FGS containing 1 mM TMZ continued to release a greater amount of TMZ over the course of the 168-h observation period (Fig.2c). Up until 48 h the ratio of released TMZ was similar at the two TMZ concentrations; a significant difference was observed after 72 h (Fig.2d). The FGS containing 1 mM TMZ continued to release TMZ gradually over the entire 168-h observation period. This shows that TMZ was released gradually and that FGS containing the higher concentration of TMZ continued to release the drug at a higher concentration and for a longer period.
Affiliation: Department of Neurosurgery, Kumamoto University Graduate School of Medical Science, Honjo, Chuo-ku, Kumamoto, Japan.