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GATA4 immunolocalization in breast carcinoma as a potent prognostic predictor.

Takagi K, Moriguchi T, Miki Y, Nakamura Y, Watanabe M, Ishida T, Yamamoto M, Sasano H, Suzuki T - Cancer Sci. (2014)

Bottom Line: GATA4 status was significantly associated with nuclear grade and van Nuys classification in DCIS and was positively associated with distant metastasis, histological grade and HER2 status, but negatively correlated with progesterone receptor labeling index in IDC.Subsequent multivariate analysis demonstrated that GATA4 status was an independent prognostic factor for both disease-free and breast cancer-specific survival of IDC patients.All of these results indicate that GATA4 plays important roles in the progression of breast carcinoma from an early stage and that immunohistochemical GATA4 status is considered a potent prognostic factor in human breast cancer patients.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology and Histotechnology, Tohoku University Graduate School of Medicine, Sendai, Japan.

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Immunohistochemistry for GATA4 in breast carcinoma. (a, b) GATA4 immunoreactivity was detected in the nucleus of carcinoma cells in ductal carcinoma in situ (DCIS) (a) and invasive ductal carcinoma (IDC) (b) tissues. (c) GATA4 immunoreactivity was not detected in the non-neoplastic mammary epithelium or stroma. (d) In the positive control section, GATA4 immunoreactivity was detected in granulosa cells of the antral follicle in the ovary. Bar, 100 μm, respectively. (e) Association between immunohistochemical GATA4 status and the signal intensity of the GATA4 gene obtained from microarray (n = 17). Data are represented as a box and whisker plot (open box, GATA4-negative group; and gray box, GATA4-positive group). The median value is represented by a horizontal line in each box and the 75th (upper margin) and 25th (lower margin) percentiles of the values are demonstrated. The upper and lower bars indicated the 90th and 10th percentiles, respectively. Statistical analysis was performed using the Mann–Whitney U-test.
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fig02: Immunohistochemistry for GATA4 in breast carcinoma. (a, b) GATA4 immunoreactivity was detected in the nucleus of carcinoma cells in ductal carcinoma in situ (DCIS) (a) and invasive ductal carcinoma (IDC) (b) tissues. (c) GATA4 immunoreactivity was not detected in the non-neoplastic mammary epithelium or stroma. (d) In the positive control section, GATA4 immunoreactivity was detected in granulosa cells of the antral follicle in the ovary. Bar, 100 μm, respectively. (e) Association between immunohistochemical GATA4 status and the signal intensity of the GATA4 gene obtained from microarray (n = 17). Data are represented as a box and whisker plot (open box, GATA4-negative group; and gray box, GATA4-positive group). The median value is represented by a horizontal line in each box and the 75th (upper margin) and 25th (lower margin) percentiles of the values are demonstrated. The upper and lower bars indicated the 90th and 10th percentiles, respectively. Statistical analysis was performed using the Mann–Whitney U-test.

Mentions: GATA4 immunoreactivity was detected in the nuclei of carcinoma cells in both DCIS (Fig.2a) and IDC (Fig.2b) tissues, but was negative in non-neoplastic mammary glands and stroma (Fig.2c). In the positive control, GATA4 was immunolocalized in the ovarian antral follicle (Fig.2d), as reported previously.21 When we immunolocalized GATA4 in 17 IDC cases using the microarray analysis, the median value of GATA4 signal intensity was 1.8-fold higher in GATA4-positive cases (n = 7) than GATA4-negative cases (n = 10), although the P-value did not reach significance (P = 0.071) (Fig.2e).


GATA4 immunolocalization in breast carcinoma as a potent prognostic predictor.

Takagi K, Moriguchi T, Miki Y, Nakamura Y, Watanabe M, Ishida T, Yamamoto M, Sasano H, Suzuki T - Cancer Sci. (2014)

Immunohistochemistry for GATA4 in breast carcinoma. (a, b) GATA4 immunoreactivity was detected in the nucleus of carcinoma cells in ductal carcinoma in situ (DCIS) (a) and invasive ductal carcinoma (IDC) (b) tissues. (c) GATA4 immunoreactivity was not detected in the non-neoplastic mammary epithelium or stroma. (d) In the positive control section, GATA4 immunoreactivity was detected in granulosa cells of the antral follicle in the ovary. Bar, 100 μm, respectively. (e) Association between immunohistochemical GATA4 status and the signal intensity of the GATA4 gene obtained from microarray (n = 17). Data are represented as a box and whisker plot (open box, GATA4-negative group; and gray box, GATA4-positive group). The median value is represented by a horizontal line in each box and the 75th (upper margin) and 25th (lower margin) percentiles of the values are demonstrated. The upper and lower bars indicated the 90th and 10th percentiles, respectively. Statistical analysis was performed using the Mann–Whitney U-test.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4317835&req=5

fig02: Immunohistochemistry for GATA4 in breast carcinoma. (a, b) GATA4 immunoreactivity was detected in the nucleus of carcinoma cells in ductal carcinoma in situ (DCIS) (a) and invasive ductal carcinoma (IDC) (b) tissues. (c) GATA4 immunoreactivity was not detected in the non-neoplastic mammary epithelium or stroma. (d) In the positive control section, GATA4 immunoreactivity was detected in granulosa cells of the antral follicle in the ovary. Bar, 100 μm, respectively. (e) Association between immunohistochemical GATA4 status and the signal intensity of the GATA4 gene obtained from microarray (n = 17). Data are represented as a box and whisker plot (open box, GATA4-negative group; and gray box, GATA4-positive group). The median value is represented by a horizontal line in each box and the 75th (upper margin) and 25th (lower margin) percentiles of the values are demonstrated. The upper and lower bars indicated the 90th and 10th percentiles, respectively. Statistical analysis was performed using the Mann–Whitney U-test.
Mentions: GATA4 immunoreactivity was detected in the nuclei of carcinoma cells in both DCIS (Fig.2a) and IDC (Fig.2b) tissues, but was negative in non-neoplastic mammary glands and stroma (Fig.2c). In the positive control, GATA4 was immunolocalized in the ovarian antral follicle (Fig.2d), as reported previously.21 When we immunolocalized GATA4 in 17 IDC cases using the microarray analysis, the median value of GATA4 signal intensity was 1.8-fold higher in GATA4-positive cases (n = 7) than GATA4-negative cases (n = 10), although the P-value did not reach significance (P = 0.071) (Fig.2e).

Bottom Line: GATA4 status was significantly associated with nuclear grade and van Nuys classification in DCIS and was positively associated with distant metastasis, histological grade and HER2 status, but negatively correlated with progesterone receptor labeling index in IDC.Subsequent multivariate analysis demonstrated that GATA4 status was an independent prognostic factor for both disease-free and breast cancer-specific survival of IDC patients.All of these results indicate that GATA4 plays important roles in the progression of breast carcinoma from an early stage and that immunohistochemical GATA4 status is considered a potent prognostic factor in human breast cancer patients.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology and Histotechnology, Tohoku University Graduate School of Medicine, Sendai, Japan.

Show MeSH
Related in: MedlinePlus