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Inhibitors of differentiation-1 promotes nitrosopyrrolidine-induced transformation of HPV 16-immortalized cervical epithelial cell.

Xie L, Li J, Zhang Y, Liu B, Peng X, Lin Y, Xu W, Hu L - Cancer Sci. (2014)

Bottom Line: Our previous study implied a correlation between inhibitors of differentiation-1 (Id-1) and cervical cancer development.However, how Id-1 contributes to cervical carcinogenesis is unknown.Our results suggest that Id-1 plays an oncogenic role in HPV-related cervical carcinogenesis, which sheds light on cervical cancer development mechanisms and implies that Id-1 is a potential target for cervical cancer prevention and therapy.

View Article: PubMed Central - PubMed

Affiliation: Department of Gynecology and Obstetrics, West China Second Hospital, Sichuan University, Chengdu, China; Laboratory of Biomedical Ultrasonics/Gynecological Oncology Laboratory, West China Second Hospital, Sichuan University, Chengdu, China.

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Evaluations of human papillomavirus E6, E7 and p53, and pRb during N-nitrosopyrrolidine-induced cell transformation. Total proteins from NPYR transformed or untreated H8 cells were subjected to 10% SDS-PAGE gels and followed by western blot. β-actin served as an input control.
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fig07: Evaluations of human papillomavirus E6, E7 and p53, and pRb during N-nitrosopyrrolidine-induced cell transformation. Total proteins from NPYR transformed or untreated H8 cells were subjected to 10% SDS-PAGE gels and followed by western blot. β-actin served as an input control.

Mentions: To explore the possible mechanism of Id-1 upregulation in NPYR-treated H8 cells, we examined the expression of the HPV E6 and E7 oncoproteins and their target tumor suppressors p53 and pRb in transformed H8 cells induced by NPYR. The results show that the expression of HPV E6 and E7 proteins was significantly increased, while that of p53 and pRb protein was decreased in the transformed cells (Fig.7).


Inhibitors of differentiation-1 promotes nitrosopyrrolidine-induced transformation of HPV 16-immortalized cervical epithelial cell.

Xie L, Li J, Zhang Y, Liu B, Peng X, Lin Y, Xu W, Hu L - Cancer Sci. (2014)

Evaluations of human papillomavirus E6, E7 and p53, and pRb during N-nitrosopyrrolidine-induced cell transformation. Total proteins from NPYR transformed or untreated H8 cells were subjected to 10% SDS-PAGE gels and followed by western blot. β-actin served as an input control.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4317834&req=5

fig07: Evaluations of human papillomavirus E6, E7 and p53, and pRb during N-nitrosopyrrolidine-induced cell transformation. Total proteins from NPYR transformed or untreated H8 cells were subjected to 10% SDS-PAGE gels and followed by western blot. β-actin served as an input control.
Mentions: To explore the possible mechanism of Id-1 upregulation in NPYR-treated H8 cells, we examined the expression of the HPV E6 and E7 oncoproteins and their target tumor suppressors p53 and pRb in transformed H8 cells induced by NPYR. The results show that the expression of HPV E6 and E7 proteins was significantly increased, while that of p53 and pRb protein was decreased in the transformed cells (Fig.7).

Bottom Line: Our previous study implied a correlation between inhibitors of differentiation-1 (Id-1) and cervical cancer development.However, how Id-1 contributes to cervical carcinogenesis is unknown.Our results suggest that Id-1 plays an oncogenic role in HPV-related cervical carcinogenesis, which sheds light on cervical cancer development mechanisms and implies that Id-1 is a potential target for cervical cancer prevention and therapy.

View Article: PubMed Central - PubMed

Affiliation: Department of Gynecology and Obstetrics, West China Second Hospital, Sichuan University, Chengdu, China; Laboratory of Biomedical Ultrasonics/Gynecological Oncology Laboratory, West China Second Hospital, Sichuan University, Chengdu, China.

Show MeSH
Related in: MedlinePlus