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Inhibitors of differentiation-1 promotes nitrosopyrrolidine-induced transformation of HPV 16-immortalized cervical epithelial cell.

Xie L, Li J, Zhang Y, Liu B, Peng X, Lin Y, Xu W, Hu L - Cancer Sci. (2014)

Bottom Line: Our previous study implied a correlation between inhibitors of differentiation-1 (Id-1) and cervical cancer development.However, how Id-1 contributes to cervical carcinogenesis is unknown.Our results suggest that Id-1 plays an oncogenic role in HPV-related cervical carcinogenesis, which sheds light on cervical cancer development mechanisms and implies that Id-1 is a potential target for cervical cancer prevention and therapy.

View Article: PubMed Central - PubMed

Affiliation: Department of Gynecology and Obstetrics, West China Second Hospital, Sichuan University, Chengdu, China; Laboratory of Biomedical Ultrasonics/Gynecological Oncology Laboratory, West China Second Hospital, Sichuan University, Chengdu, China.

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Increased inhibitors of differentiation-1 (Id-1) expression in N-nitrosopyrrolidine (NPYR)-transformed H8 cells. (a) Total proteins extracted from H8, NPYR-H8 and SiHa cells were subjected to 10% SDS-PAGE gels and assayed by western blot. (b) Id-1 RNA levels in H8, NPYR-H8 and SiHa cells were detected by qRT-PCR. Expression of Id-1 gene, in each sample was normalized against the B2M endogenous control. The normalized values were then calibrated against the H8 cell values that were arbitrarily set as 1.
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fig03: Increased inhibitors of differentiation-1 (Id-1) expression in N-nitrosopyrrolidine (NPYR)-transformed H8 cells. (a) Total proteins extracted from H8, NPYR-H8 and SiHa cells were subjected to 10% SDS-PAGE gels and assayed by western blot. (b) Id-1 RNA levels in H8, NPYR-H8 and SiHa cells were detected by qRT-PCR. Expression of Id-1 gene, in each sample was normalized against the B2M endogenous control. The normalized values were then calibrated against the H8 cell values that were arbitrarily set as 1.

Mentions: We investigated Id-1 expression at both protein and RNA levels in parental and transformed H8 cells. SiHa cells were used as a positive control. A significant increase of Id-1 protein expression in transformed H8 cells compared to the parental H8 cells was detected by western blot (Fig.3a). By quantitative RT-PCR, the Id-1 RNA level in transformed H8 cells was found to be 3.8-fold higher than that in the untransformed H8 cells (Fig.3b, P < 0.05).


Inhibitors of differentiation-1 promotes nitrosopyrrolidine-induced transformation of HPV 16-immortalized cervical epithelial cell.

Xie L, Li J, Zhang Y, Liu B, Peng X, Lin Y, Xu W, Hu L - Cancer Sci. (2014)

Increased inhibitors of differentiation-1 (Id-1) expression in N-nitrosopyrrolidine (NPYR)-transformed H8 cells. (a) Total proteins extracted from H8, NPYR-H8 and SiHa cells were subjected to 10% SDS-PAGE gels and assayed by western blot. (b) Id-1 RNA levels in H8, NPYR-H8 and SiHa cells were detected by qRT-PCR. Expression of Id-1 gene, in each sample was normalized against the B2M endogenous control. The normalized values were then calibrated against the H8 cell values that were arbitrarily set as 1.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4317834&req=5

fig03: Increased inhibitors of differentiation-1 (Id-1) expression in N-nitrosopyrrolidine (NPYR)-transformed H8 cells. (a) Total proteins extracted from H8, NPYR-H8 and SiHa cells were subjected to 10% SDS-PAGE gels and assayed by western blot. (b) Id-1 RNA levels in H8, NPYR-H8 and SiHa cells were detected by qRT-PCR. Expression of Id-1 gene, in each sample was normalized against the B2M endogenous control. The normalized values were then calibrated against the H8 cell values that were arbitrarily set as 1.
Mentions: We investigated Id-1 expression at both protein and RNA levels in parental and transformed H8 cells. SiHa cells were used as a positive control. A significant increase of Id-1 protein expression in transformed H8 cells compared to the parental H8 cells was detected by western blot (Fig.3a). By quantitative RT-PCR, the Id-1 RNA level in transformed H8 cells was found to be 3.8-fold higher than that in the untransformed H8 cells (Fig.3b, P < 0.05).

Bottom Line: Our previous study implied a correlation between inhibitors of differentiation-1 (Id-1) and cervical cancer development.However, how Id-1 contributes to cervical carcinogenesis is unknown.Our results suggest that Id-1 plays an oncogenic role in HPV-related cervical carcinogenesis, which sheds light on cervical cancer development mechanisms and implies that Id-1 is a potential target for cervical cancer prevention and therapy.

View Article: PubMed Central - PubMed

Affiliation: Department of Gynecology and Obstetrics, West China Second Hospital, Sichuan University, Chengdu, China; Laboratory of Biomedical Ultrasonics/Gynecological Oncology Laboratory, West China Second Hospital, Sichuan University, Chengdu, China.

Show MeSH
Related in: MedlinePlus