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Inhibitors of differentiation-1 promotes nitrosopyrrolidine-induced transformation of HPV 16-immortalized cervical epithelial cell.

Xie L, Li J, Zhang Y, Liu B, Peng X, Lin Y, Xu W, Hu L - Cancer Sci. (2014)

Bottom Line: Our previous study implied a correlation between inhibitors of differentiation-1 (Id-1) and cervical cancer development.However, how Id-1 contributes to cervical carcinogenesis is unknown.Our results suggest that Id-1 plays an oncogenic role in HPV-related cervical carcinogenesis, which sheds light on cervical cancer development mechanisms and implies that Id-1 is a potential target for cervical cancer prevention and therapy.

View Article: PubMed Central - PubMed

Affiliation: Department of Gynecology and Obstetrics, West China Second Hospital, Sichuan University, Chengdu, China; Laboratory of Biomedical Ultrasonics/Gynecological Oncology Laboratory, West China Second Hospital, Sichuan University, Chengdu, China.

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Tumorigenicity of N-nitrosopyrrolidine (NPYR)-transformed H8 cells in nude mice. Nude mice were injected (s.c.) with H8 cells, NPYR transformed H8 (NPYR-H8) cells or SiHa cells, and were photographed 8 weeks after injection. Tumor size of each group is shown (n = 10). (a) One representative mouse from each group is shown. Injection sites are indicated by arrowheads. (b) Tumor growth curves of the NPYR-H8 and SiHa groups.
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fig02: Tumorigenicity of N-nitrosopyrrolidine (NPYR)-transformed H8 cells in nude mice. Nude mice were injected (s.c.) with H8 cells, NPYR transformed H8 (NPYR-H8) cells or SiHa cells, and were photographed 8 weeks after injection. Tumor size of each group is shown (n = 10). (a) One representative mouse from each group is shown. Injection sites are indicated by arrowheads. (b) Tumor growth curves of the NPYR-H8 and SiHa groups.

Mentions: The HPV-16 immortalized but not transformed H8 cells resemble normal human cervical epithelial cells but are not transformed by the HPV E6/E7 oncogene. We first confirmed that the H8 cells lack the capacities in anchorage-independent growth in soft agar and tumor-formation in nude mice, the malignant characteristics of cancer cells. The cervical cancer cell line SiHa was used as a positive control (Figs1 and 2). After treatment with NYPR for 4 weeks, the cells became capable of forming colonies in soft agar (Fig.1) and tumors in nude mice (Fig.2a). The tumor growth curve also showed a parallel trend between SiHa and transformed H8 groups (Fig.2b), suggesting that the cells were transformed by NPYR. Then the NYPR exposure protocol was used to assay the role of Id-1 in H8 transformation by NYPR.


Inhibitors of differentiation-1 promotes nitrosopyrrolidine-induced transformation of HPV 16-immortalized cervical epithelial cell.

Xie L, Li J, Zhang Y, Liu B, Peng X, Lin Y, Xu W, Hu L - Cancer Sci. (2014)

Tumorigenicity of N-nitrosopyrrolidine (NPYR)-transformed H8 cells in nude mice. Nude mice were injected (s.c.) with H8 cells, NPYR transformed H8 (NPYR-H8) cells or SiHa cells, and were photographed 8 weeks after injection. Tumor size of each group is shown (n = 10). (a) One representative mouse from each group is shown. Injection sites are indicated by arrowheads. (b) Tumor growth curves of the NPYR-H8 and SiHa groups.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4317834&req=5

fig02: Tumorigenicity of N-nitrosopyrrolidine (NPYR)-transformed H8 cells in nude mice. Nude mice were injected (s.c.) with H8 cells, NPYR transformed H8 (NPYR-H8) cells or SiHa cells, and were photographed 8 weeks after injection. Tumor size of each group is shown (n = 10). (a) One representative mouse from each group is shown. Injection sites are indicated by arrowheads. (b) Tumor growth curves of the NPYR-H8 and SiHa groups.
Mentions: The HPV-16 immortalized but not transformed H8 cells resemble normal human cervical epithelial cells but are not transformed by the HPV E6/E7 oncogene. We first confirmed that the H8 cells lack the capacities in anchorage-independent growth in soft agar and tumor-formation in nude mice, the malignant characteristics of cancer cells. The cervical cancer cell line SiHa was used as a positive control (Figs1 and 2). After treatment with NYPR for 4 weeks, the cells became capable of forming colonies in soft agar (Fig.1) and tumors in nude mice (Fig.2a). The tumor growth curve also showed a parallel trend between SiHa and transformed H8 groups (Fig.2b), suggesting that the cells were transformed by NPYR. Then the NYPR exposure protocol was used to assay the role of Id-1 in H8 transformation by NYPR.

Bottom Line: Our previous study implied a correlation between inhibitors of differentiation-1 (Id-1) and cervical cancer development.However, how Id-1 contributes to cervical carcinogenesis is unknown.Our results suggest that Id-1 plays an oncogenic role in HPV-related cervical carcinogenesis, which sheds light on cervical cancer development mechanisms and implies that Id-1 is a potential target for cervical cancer prevention and therapy.

View Article: PubMed Central - PubMed

Affiliation: Department of Gynecology and Obstetrics, West China Second Hospital, Sichuan University, Chengdu, China; Laboratory of Biomedical Ultrasonics/Gynecological Oncology Laboratory, West China Second Hospital, Sichuan University, Chengdu, China.

Show MeSH
Related in: MedlinePlus