Limits...
Inhibitors of differentiation-1 promotes nitrosopyrrolidine-induced transformation of HPV 16-immortalized cervical epithelial cell.

Xie L, Li J, Zhang Y, Liu B, Peng X, Lin Y, Xu W, Hu L - Cancer Sci. (2014)

Bottom Line: Our previous study implied a correlation between inhibitors of differentiation-1 (Id-1) and cervical cancer development.However, how Id-1 contributes to cervical carcinogenesis is unknown.Our results suggest that Id-1 plays an oncogenic role in HPV-related cervical carcinogenesis, which sheds light on cervical cancer development mechanisms and implies that Id-1 is a potential target for cervical cancer prevention and therapy.

View Article: PubMed Central - PubMed

Affiliation: Department of Gynecology and Obstetrics, West China Second Hospital, Sichuan University, Chengdu, China; Laboratory of Biomedical Ultrasonics/Gynecological Oncology Laboratory, West China Second Hospital, Sichuan University, Chengdu, China.

Show MeSH

Related in: MedlinePlus

Establishment of N-nitrosopyrrolidine (NPYR)-induced transformation in H8 cells. Cells treated with NPYR were seeded in soft agar, and colony numbers were counted 2 weeks after seeding. Untreated H8 cells and SiHa cells were seeded as negative and positive controls, respectively. (a) Representative images taken under a light microscope (40×). (b) Colony numbers in each group counted in five fields (mean ± SD).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4317834&req=5

fig01: Establishment of N-nitrosopyrrolidine (NPYR)-induced transformation in H8 cells. Cells treated with NPYR were seeded in soft agar, and colony numbers were counted 2 weeks after seeding. Untreated H8 cells and SiHa cells were seeded as negative and positive controls, respectively. (a) Representative images taken under a light microscope (40×). (b) Colony numbers in each group counted in five fields (mean ± SD).

Mentions: The HPV-16 immortalized but not transformed H8 cells resemble normal human cervical epithelial cells but are not transformed by the HPV E6/E7 oncogene. We first confirmed that the H8 cells lack the capacities in anchorage-independent growth in soft agar and tumor-formation in nude mice, the malignant characteristics of cancer cells. The cervical cancer cell line SiHa was used as a positive control (Figs1 and 2). After treatment with NYPR for 4 weeks, the cells became capable of forming colonies in soft agar (Fig.1) and tumors in nude mice (Fig.2a). The tumor growth curve also showed a parallel trend between SiHa and transformed H8 groups (Fig.2b), suggesting that the cells were transformed by NPYR. Then the NYPR exposure protocol was used to assay the role of Id-1 in H8 transformation by NYPR.


Inhibitors of differentiation-1 promotes nitrosopyrrolidine-induced transformation of HPV 16-immortalized cervical epithelial cell.

Xie L, Li J, Zhang Y, Liu B, Peng X, Lin Y, Xu W, Hu L - Cancer Sci. (2014)

Establishment of N-nitrosopyrrolidine (NPYR)-induced transformation in H8 cells. Cells treated with NPYR were seeded in soft agar, and colony numbers were counted 2 weeks after seeding. Untreated H8 cells and SiHa cells were seeded as negative and positive controls, respectively. (a) Representative images taken under a light microscope (40×). (b) Colony numbers in each group counted in five fields (mean ± SD).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4317834&req=5

fig01: Establishment of N-nitrosopyrrolidine (NPYR)-induced transformation in H8 cells. Cells treated with NPYR were seeded in soft agar, and colony numbers were counted 2 weeks after seeding. Untreated H8 cells and SiHa cells were seeded as negative and positive controls, respectively. (a) Representative images taken under a light microscope (40×). (b) Colony numbers in each group counted in five fields (mean ± SD).
Mentions: The HPV-16 immortalized but not transformed H8 cells resemble normal human cervical epithelial cells but are not transformed by the HPV E6/E7 oncogene. We first confirmed that the H8 cells lack the capacities in anchorage-independent growth in soft agar and tumor-formation in nude mice, the malignant characteristics of cancer cells. The cervical cancer cell line SiHa was used as a positive control (Figs1 and 2). After treatment with NYPR for 4 weeks, the cells became capable of forming colonies in soft agar (Fig.1) and tumors in nude mice (Fig.2a). The tumor growth curve also showed a parallel trend between SiHa and transformed H8 groups (Fig.2b), suggesting that the cells were transformed by NPYR. Then the NYPR exposure protocol was used to assay the role of Id-1 in H8 transformation by NYPR.

Bottom Line: Our previous study implied a correlation between inhibitors of differentiation-1 (Id-1) and cervical cancer development.However, how Id-1 contributes to cervical carcinogenesis is unknown.Our results suggest that Id-1 plays an oncogenic role in HPV-related cervical carcinogenesis, which sheds light on cervical cancer development mechanisms and implies that Id-1 is a potential target for cervical cancer prevention and therapy.

View Article: PubMed Central - PubMed

Affiliation: Department of Gynecology and Obstetrics, West China Second Hospital, Sichuan University, Chengdu, China; Laboratory of Biomedical Ultrasonics/Gynecological Oncology Laboratory, West China Second Hospital, Sichuan University, Chengdu, China.

Show MeSH
Related in: MedlinePlus