Diagnostic approach for cancer cells in urine sediments by 5-aminolevulinic acid-based photodynamic detection in bladder cancer.
Bottom Line: The overall sensitivities of conventional cytology, BTA, NMP22, fluorescence cytology, fluorescent spectrophotometric assay and CFAU assay were 48%, 33%, 40%, 86%, 86% and 87%, respectively.Three different ALA-based assays showed high sensitivity and specificity.Development of a rapid and automated device for ALA-based photodynamic assay is necessary to avoid the variability induced by troublesome steps and low stability of specimens.
Affiliation: Department of Urology, Nara Medical University, Kashihara-shi, Nara, Japan.Show MeSH
Related in: MedlinePlus
Mentions: The overall sensitivity values for conventional cytology, BTA, NMP22, fluorescence cytology, fluorescent spectrophotometric assay and CFAU assay were 48%, 33%, 40%, 86%, 86% and 87%, respectively (Table1). BTA and NMP22 were less sensitive compared to conventional cytology and fluorescence-based assays. Three different fluorescence-based assays showed similar sensitivity. Next, the results of conventional cytology, fluorescence cytology and fluorescent spectrophotometric assay were compared using contingency tables (Fig.6). Fluorescence cytology detected 34 cancers that conventional cytology failed to detect (Fig.6a). Only two cases were detected by conventional cytology that fluorescence cytology failed to detect. Fluorescence spectrophotometric assay detected 32 cases that conventional cytology failed to detect (Fig.6b). The results of fluorescence cytology and fluorescence spectrophotometric assay were compared, and a high concordance (86%, 48 of 58 cases) was demonstrated between the two different assays (Fig.6c).
Affiliation: Department of Urology, Nara Medical University, Kashihara-shi, Nara, Japan.