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Diagnostic approach for cancer cells in urine sediments by 5-aminolevulinic acid-based photodynamic detection in bladder cancer.

Miyake M, Nakai Y, Anai S, Tatsumi Y, Kuwada M, Onishi S, Chihara Y, Tanaka N, Hirao Y, Fujimoto K - Cancer Sci. (2014)

Bottom Line: The overall sensitivities of conventional cytology, BTA, NMP22, fluorescence cytology, fluorescent spectrophotometric assay and CFAU assay were 48%, 33%, 40%, 86%, 86% and 87%, respectively.Three different ALA-based assays showed high sensitivity and specificity.Development of a rapid and automated device for ALA-based photodynamic assay is necessary to avoid the variability induced by troublesome steps and low stability of specimens.

View Article: PubMed Central - PubMed

Affiliation: Department of Urology, Nara Medical University, Kashihara-shi, Nara, Japan.

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Related in: MedlinePlus

Detection of urothelial carcinoma cells by fluorescence microscopy. Cell suspension of ALA-treated urine sediments in PBS was transferred onto a microscope slide and covered using a cover slip. Cells were observed with regular light microscopy to find urothelial cells and exclude non-epithelial cells such as red blood cells. Representative photographs of T24 cells, pT1 HG bladder cancer, benign prostate hyperplasia (BPH), and chronic kidney disease (CKD) are shown. Blue arrowheads indicate red fluorescence-positive cells.
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fig02: Detection of urothelial carcinoma cells by fluorescence microscopy. Cell suspension of ALA-treated urine sediments in PBS was transferred onto a microscope slide and covered using a cover slip. Cells were observed with regular light microscopy to find urothelial cells and exclude non-epithelial cells such as red blood cells. Representative photographs of T24 cells, pT1 HG bladder cancer, benign prostate hyperplasia (BPH), and chronic kidney disease (CKD) are shown. Blue arrowheads indicate red fluorescence-positive cells.

Mentions: Using this method, PPIX red fluorescence was localized in the cytoplasm and/or nucleus of urothelial cells. Identification of UC cells was based on the morphologic criteria of malignancy, such as enlarged polymorphous cells with prominent lobulated nuclei and high nucleus/cytoplasm ratio. In each test, T24 cells treated with ALA for 2 h were used as a positive control. Exposure time and gain were adjusted so that T24 cells treated with ALA looked bright red and the background was completely black (Fig.2, upper panels). Fluorescence cytology was considered positive if the red fluorescence was detected in urothelial cells (Fig.2). The results were compared with the tumor histology, stage and findings of conventional cytological examination. The examiner had no clinical information prior to the examination.


Diagnostic approach for cancer cells in urine sediments by 5-aminolevulinic acid-based photodynamic detection in bladder cancer.

Miyake M, Nakai Y, Anai S, Tatsumi Y, Kuwada M, Onishi S, Chihara Y, Tanaka N, Hirao Y, Fujimoto K - Cancer Sci. (2014)

Detection of urothelial carcinoma cells by fluorescence microscopy. Cell suspension of ALA-treated urine sediments in PBS was transferred onto a microscope slide and covered using a cover slip. Cells were observed with regular light microscopy to find urothelial cells and exclude non-epithelial cells such as red blood cells. Representative photographs of T24 cells, pT1 HG bladder cancer, benign prostate hyperplasia (BPH), and chronic kidney disease (CKD) are shown. Blue arrowheads indicate red fluorescence-positive cells.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4317833&req=5

fig02: Detection of urothelial carcinoma cells by fluorescence microscopy. Cell suspension of ALA-treated urine sediments in PBS was transferred onto a microscope slide and covered using a cover slip. Cells were observed with regular light microscopy to find urothelial cells and exclude non-epithelial cells such as red blood cells. Representative photographs of T24 cells, pT1 HG bladder cancer, benign prostate hyperplasia (BPH), and chronic kidney disease (CKD) are shown. Blue arrowheads indicate red fluorescence-positive cells.
Mentions: Using this method, PPIX red fluorescence was localized in the cytoplasm and/or nucleus of urothelial cells. Identification of UC cells was based on the morphologic criteria of malignancy, such as enlarged polymorphous cells with prominent lobulated nuclei and high nucleus/cytoplasm ratio. In each test, T24 cells treated with ALA for 2 h were used as a positive control. Exposure time and gain were adjusted so that T24 cells treated with ALA looked bright red and the background was completely black (Fig.2, upper panels). Fluorescence cytology was considered positive if the red fluorescence was detected in urothelial cells (Fig.2). The results were compared with the tumor histology, stage and findings of conventional cytological examination. The examiner had no clinical information prior to the examination.

Bottom Line: The overall sensitivities of conventional cytology, BTA, NMP22, fluorescence cytology, fluorescent spectrophotometric assay and CFAU assay were 48%, 33%, 40%, 86%, 86% and 87%, respectively.Three different ALA-based assays showed high sensitivity and specificity.Development of a rapid and automated device for ALA-based photodynamic assay is necessary to avoid the variability induced by troublesome steps and low stability of specimens.

View Article: PubMed Central - PubMed

Affiliation: Department of Urology, Nara Medical University, Kashihara-shi, Nara, Japan.

Show MeSH
Related in: MedlinePlus