Diagnostic approach for cancer cells in urine sediments by 5-aminolevulinic acid-based photodynamic detection in bladder cancer.
Bottom Line: The overall sensitivities of conventional cytology, BTA, NMP22, fluorescence cytology, fluorescent spectrophotometric assay and CFAU assay were 48%, 33%, 40%, 86%, 86% and 87%, respectively.Three different ALA-based assays showed high sensitivity and specificity.Development of a rapid and automated device for ALA-based photodynamic assay is necessary to avoid the variability induced by troublesome steps and low stability of specimens.
Affiliation: Department of Urology, Nara Medical University, Kashihara-shi, Nara, Japan.Show MeSH
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Mentions: Using this method, PPIX red fluorescence was localized in the cytoplasm and/or nucleus of urothelial cells. Identification of UC cells was based on the morphologic criteria of malignancy, such as enlarged polymorphous cells with prominent lobulated nuclei and high nucleus/cytoplasm ratio. In each test, T24 cells treated with ALA for 2 h were used as a positive control. Exposure time and gain were adjusted so that T24 cells treated with ALA looked bright red and the background was completely black (Fig.2, upper panels). Fluorescence cytology was considered positive if the red fluorescence was detected in urothelial cells (Fig.2). The results were compared with the tumor histology, stage and findings of conventional cytological examination. The examiner had no clinical information prior to the examination.
Affiliation: Department of Urology, Nara Medical University, Kashihara-shi, Nara, Japan.