Diagnostic approach for cancer cells in urine sediments by 5-aminolevulinic acid-based photodynamic detection in bladder cancer.
Bottom Line: The overall sensitivities of conventional cytology, BTA, NMP22, fluorescence cytology, fluorescent spectrophotometric assay and CFAU assay were 48%, 33%, 40%, 86%, 86% and 87%, respectively.Three different ALA-based assays showed high sensitivity and specificity.Development of a rapid and automated device for ALA-based photodynamic assay is necessary to avoid the variability induced by troublesome steps and low stability of specimens.
Affiliation: Department of Urology, Nara Medical University, Kashihara-shi, Nara, Japan.Show MeSH
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Mentions: Before TURBT, 200–300 mL of voided urine was obtained for conventional cytology, a urine BTA test, a urine NMP22 test and fluorescence cytology. Fluorescence cytology was performed within 2 h after sample collection using the following procedures (Fig.1). Voided urine samples obtained from 20 benign cases were used as controls. Voided urine was centrifuged for 5 min at 400g. The cell pellet was washed using PBS. After centrifugation, the pellet was resuspended in serum-free culture medium, RPMI-1640 containing 1 mM of ALA. The cell suspension was then incubated for 2 h at 37°C to allow cells to produce and accumulate intracellular PPIX. After treatment with ALA, subsequent procedures were performed under light-protected conditions.
Affiliation: Department of Urology, Nara Medical University, Kashihara-shi, Nara, Japan.