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Effect of a poly(ADP-ribose) polymerase-1 inhibitor against esophageal squamous cell carcinoma cell lines.

Nasuno T, Mimaki S, Okamoto M, Esumi H, Tsuchihara K - Cancer Sci. (2013)

Bottom Line: Effective molecular target drugs that improve therapeutic efficacy with fewer adverse effects for esophageal cancer are highly anticipated.Of these eight cell lines, the clonogenic survival of one (TE-6) was reduced by AZD2281 to the level of DSB repair-defective Capan-1 and HCC1937 cells.Interestingly, a strong correlation between basal expression levels of γ-H2AX and sensitivity to AZD2281was observed in the TE-series cells (R(2)  = 0.5345).

View Article: PubMed Central - PubMed

Affiliation: Division of Translational Research, Exploratory Oncology Research and Clinical Trial Center, National Cancer Center, Kashiwa, Chiba, Japan; Department of Otorhinolaryngology, University of Kitasato Hospital, Minami-ku, Sagamihara, Kanagawa, Japan.

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A strong correlation between base-level γ-H2AX and sensitivity to AZD2281 of TE cells. (a) Eight non-treated TE-series cell lines were subjected to Western blot analysis with antibodies against γ-H2AX and actin. (b) The correlation between basal γ-H2AX expression levels and IC50 of AZD2281. The average intensity of γ-H2AX was standardized with actin. The data represent the averages and standard deviations of three independent experiments.
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fig04: A strong correlation between base-level γ-H2AX and sensitivity to AZD2281 of TE cells. (a) Eight non-treated TE-series cell lines were subjected to Western blot analysis with antibodies against γ-H2AX and actin. (b) The correlation between basal γ-H2AX expression levels and IC50 of AZD2281. The average intensity of γ-H2AX was standardized with actin. The data represent the averages and standard deviations of three independent experiments.

Mentions: The Western blotting and immunofluorescence data also suggested that the baseline γ-H2AX level was higher in the AZD2281-sensitive TE-6 cells than in the resistant TE-1 cells (Fig. 3a). Because the increased amount of γ-H2AX may suggest the accumulation of DSBs, we evaluated the correlation between the basal expression levels of γ-H2AX and sensitivity to AZD2281 among the eight TE cell lines. A significant correlation between the basal levels of γ-H2AX and the IC50 of AZD2281 was observed (R2 = 0.5345) (Fig. 4a,b).


Effect of a poly(ADP-ribose) polymerase-1 inhibitor against esophageal squamous cell carcinoma cell lines.

Nasuno T, Mimaki S, Okamoto M, Esumi H, Tsuchihara K - Cancer Sci. (2013)

A strong correlation between base-level γ-H2AX and sensitivity to AZD2281 of TE cells. (a) Eight non-treated TE-series cell lines were subjected to Western blot analysis with antibodies against γ-H2AX and actin. (b) The correlation between basal γ-H2AX expression levels and IC50 of AZD2281. The average intensity of γ-H2AX was standardized with actin. The data represent the averages and standard deviations of three independent experiments.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4317825&req=5

fig04: A strong correlation between base-level γ-H2AX and sensitivity to AZD2281 of TE cells. (a) Eight non-treated TE-series cell lines were subjected to Western blot analysis with antibodies against γ-H2AX and actin. (b) The correlation between basal γ-H2AX expression levels and IC50 of AZD2281. The average intensity of γ-H2AX was standardized with actin. The data represent the averages and standard deviations of three independent experiments.
Mentions: The Western blotting and immunofluorescence data also suggested that the baseline γ-H2AX level was higher in the AZD2281-sensitive TE-6 cells than in the resistant TE-1 cells (Fig. 3a). Because the increased amount of γ-H2AX may suggest the accumulation of DSBs, we evaluated the correlation between the basal expression levels of γ-H2AX and sensitivity to AZD2281 among the eight TE cell lines. A significant correlation between the basal levels of γ-H2AX and the IC50 of AZD2281 was observed (R2 = 0.5345) (Fig. 4a,b).

Bottom Line: Effective molecular target drugs that improve therapeutic efficacy with fewer adverse effects for esophageal cancer are highly anticipated.Of these eight cell lines, the clonogenic survival of one (TE-6) was reduced by AZD2281 to the level of DSB repair-defective Capan-1 and HCC1937 cells.Interestingly, a strong correlation between basal expression levels of γ-H2AX and sensitivity to AZD2281was observed in the TE-series cells (R(2)  = 0.5345).

View Article: PubMed Central - PubMed

Affiliation: Division of Translational Research, Exploratory Oncology Research and Clinical Trial Center, National Cancer Center, Kashiwa, Chiba, Japan; Department of Otorhinolaryngology, University of Kitasato Hospital, Minami-ku, Sagamihara, Kanagawa, Japan.

Show MeSH
Related in: MedlinePlus