Limits...
Effect of a poly(ADP-ribose) polymerase-1 inhibitor against esophageal squamous cell carcinoma cell lines.

Nasuno T, Mimaki S, Okamoto M, Esumi H, Tsuchihara K - Cancer Sci. (2013)

Bottom Line: Effective molecular target drugs that improve therapeutic efficacy with fewer adverse effects for esophageal cancer are highly anticipated.Of these eight cell lines, the clonogenic survival of one (TE-6) was reduced by AZD2281 to the level of DSB repair-defective Capan-1 and HCC1937 cells.Interestingly, a strong correlation between basal expression levels of γ-H2AX and sensitivity to AZD2281was observed in the TE-series cells (R(2)  = 0.5345).

View Article: PubMed Central - PubMed

Affiliation: Division of Translational Research, Exploratory Oncology Research and Clinical Trial Center, National Cancer Center, Kashiwa, Chiba, Japan; Department of Otorhinolaryngology, University of Kitasato Hospital, Minami-ku, Sagamihara, Kanagawa, Japan.

Show MeSH

Related in: MedlinePlus

AZD2281-induced G2/M arrest in TE-6 cells. (a) TE-6 and TE-1 cells were cultured with or without AZD2281 for 24 h. DNA ploidy was assessed by propidium iodide (PI) staining and flow cytometry. (b) The proportion of estimated cell-cycle phases in TE-6 and TE-1 cells treated with or without AZD2281. The data represent the average of three independent experiments.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4317825&req=5

fig02: AZD2281-induced G2/M arrest in TE-6 cells. (a) TE-6 and TE-1 cells were cultured with or without AZD2281 for 24 h. DNA ploidy was assessed by propidium iodide (PI) staining and flow cytometry. (b) The proportion of estimated cell-cycle phases in TE-6 and TE-1 cells treated with or without AZD2281. The data represent the average of three independent experiments.

Mentions: To further study the mechanism of growth retardation of TE-6 cells by AZD2281, the status of the cell cycle in these cells was assessed by analyzing the DNA ploidy pattern (Figs 2a,b, S3). Treatment with 1 and 5 μM of AZD2281 for 12 h increased the population with 4n DNA content from 36.7% to 40.7% and 40.6%, respectively. Treatment for 24 h further increased the population with 4n DNA content from 31.6% to 37.9% and 46.3%, respectively. This suggested an increase in the G2/M or M arrested population after AZD2281 treatment. On the other hand, no significant increase of tetraploid cells was observed in the TE-1 cells.


Effect of a poly(ADP-ribose) polymerase-1 inhibitor against esophageal squamous cell carcinoma cell lines.

Nasuno T, Mimaki S, Okamoto M, Esumi H, Tsuchihara K - Cancer Sci. (2013)

AZD2281-induced G2/M arrest in TE-6 cells. (a) TE-6 and TE-1 cells were cultured with or without AZD2281 for 24 h. DNA ploidy was assessed by propidium iodide (PI) staining and flow cytometry. (b) The proportion of estimated cell-cycle phases in TE-6 and TE-1 cells treated with or without AZD2281. The data represent the average of three independent experiments.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4317825&req=5

fig02: AZD2281-induced G2/M arrest in TE-6 cells. (a) TE-6 and TE-1 cells were cultured with or without AZD2281 for 24 h. DNA ploidy was assessed by propidium iodide (PI) staining and flow cytometry. (b) The proportion of estimated cell-cycle phases in TE-6 and TE-1 cells treated with or without AZD2281. The data represent the average of three independent experiments.
Mentions: To further study the mechanism of growth retardation of TE-6 cells by AZD2281, the status of the cell cycle in these cells was assessed by analyzing the DNA ploidy pattern (Figs 2a,b, S3). Treatment with 1 and 5 μM of AZD2281 for 12 h increased the population with 4n DNA content from 36.7% to 40.7% and 40.6%, respectively. Treatment for 24 h further increased the population with 4n DNA content from 31.6% to 37.9% and 46.3%, respectively. This suggested an increase in the G2/M or M arrested population after AZD2281 treatment. On the other hand, no significant increase of tetraploid cells was observed in the TE-1 cells.

Bottom Line: Effective molecular target drugs that improve therapeutic efficacy with fewer adverse effects for esophageal cancer are highly anticipated.Of these eight cell lines, the clonogenic survival of one (TE-6) was reduced by AZD2281 to the level of DSB repair-defective Capan-1 and HCC1937 cells.Interestingly, a strong correlation between basal expression levels of γ-H2AX and sensitivity to AZD2281was observed in the TE-series cells (R(2)  = 0.5345).

View Article: PubMed Central - PubMed

Affiliation: Division of Translational Research, Exploratory Oncology Research and Clinical Trial Center, National Cancer Center, Kashiwa, Chiba, Japan; Department of Otorhinolaryngology, University of Kitasato Hospital, Minami-ku, Sagamihara, Kanagawa, Japan.

Show MeSH
Related in: MedlinePlus