Apurinic/apyrimidinic endonuclease 1 induced upregulation of fibroblast growth factor 2 and its receptor 3 induces angiogenesis in human osteosarcoma cells.
Bottom Line: Our preliminary data show small interfering RNA-mediated silence of APE1 experiments, which further supports this hypothesis.APE1-small interfering RNA significantly inhibited tumor angiogenesis by downregulating in vitro expression of FGF2 and FGFR3 in human umbilical vein endothelial cells in Matrigel tube formation assay, and further inhibited tumor growth in vivo in a mouse xenograft model.Thus, the proposed APE1-FGF2 and FGFR3 pathway may provide a novel mechanism for regulation of FGF2 and FGFR3 by APE1 in tumor angiogenesis.
Affiliation: Cancer Center, Research Institute of Surgery, Daping Hospital, Third Military Medical University, Chongqing, China.Show MeSH
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Mentions: The correlation between the expression of APE1, FGF2/FGFR3 and MVD outlined in Table 1 shows that APE1 and FGF2/FGFR3 are significantly associated with MVD when univariate analysis and the Cox model are used to investigate prognostic factors. The overall survival analysis of osteosarcoma patients shows that the 2-year and 5-year survival rates were 33.8% and 18.3%, respectively (Fig. 2). To investigate the correlation between the prognosis and the clinco-pathological factors, such as age, gender, histopathology, Enneking Staging and tumor size, APE1, FGF2/FGFR3 and MVD, a log-rank test was carried out for univariate analysis.
Affiliation: Cancer Center, Research Institute of Surgery, Daping Hospital, Third Military Medical University, Chongqing, China.