Limits...
Role of plasmacytoid dendritic cells and inducible costimulator-positive regulatory T cells in the immunosuppression microenvironment of gastric cancer.

Huang XM, Liu XS, Lin XK, Yu H, Sun JY, Liu XK, Chen C, Jin HL, Zhang GE, Shi XX, Zhang Q, Yu JR - Cancer Sci. (2014)

Bottom Line: We found that the numbers of pDCs, Tregs, and ICOS(+) Tregs in peripheral blood were increased in GC patients compared with healthy donors.In tissue, Tregs and ICOS(+) Tregs were found distributing mainly in carcinoma tissue, whereas pDCs were mainly found in peritumor tissue.In conclusion, pDCs may play a potential role in recruiting ICOS(+) Tregs, and both participate in the immunosuppression microenvironment of GC.

View Article: PubMed Central - PubMed

Affiliation: Department of Gastrointestinal Surgery, The First Affiliated Hospital, Medical College, Zhejiang University, Hangzhou, China.

Show MeSH

Related in: MedlinePlus

Characterization and quantification of blood plasmacytoid dendritic cells (pDCs). (a) PBMCs isolated from a gastric cancer (GC) patient and a control subject were analyzed for pDC levels by flow cytometry. The first gate was set on lymphocytes and monocytes (P1) and the second gate was set on Lineage− and HLA-DR+ cells (Q1). The pDCs were identified as Lineage−HLA-DR+CD123brightCD11c− cells. (b) Cumulative percentages of pDCs in Lineage− cells of the GC patient and the control subject. The prevalence of pDCs was significantly higher in the GC patient than that in the control donor. (c) The presence of pDCs was significantly associated with an increased number of ICOS+ regulatory T cells (Tregs) in peripheral blood. FSC, forward scatter; SSC, side scatter.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4317822&req=5

fig03: Characterization and quantification of blood plasmacytoid dendritic cells (pDCs). (a) PBMCs isolated from a gastric cancer (GC) patient and a control subject were analyzed for pDC levels by flow cytometry. The first gate was set on lymphocytes and monocytes (P1) and the second gate was set on Lineage− and HLA-DR+ cells (Q1). The pDCs were identified as Lineage−HLA-DR+CD123brightCD11c− cells. (b) Cumulative percentages of pDCs in Lineage− cells of the GC patient and the control subject. The prevalence of pDCs was significantly higher in the GC patient than that in the control donor. (c) The presence of pDCs was significantly associated with an increased number of ICOS+ regulatory T cells (Tregs) in peripheral blood. FSC, forward scatter; SSC, side scatter.

Mentions: Plasmacytoid dendritic cells were identified as Lin−HLA-DR+CD11c−CD123high cells (Fig. 3a). The proportion of circulating pDCs among Lineage− cells was significantly higher in GC patients than in control donors (2.64% [range, 0.14–10.70%] vs 1.51% [range, 0.11–6.91%], P = 0.027; Fig. 3b).


Role of plasmacytoid dendritic cells and inducible costimulator-positive regulatory T cells in the immunosuppression microenvironment of gastric cancer.

Huang XM, Liu XS, Lin XK, Yu H, Sun JY, Liu XK, Chen C, Jin HL, Zhang GE, Shi XX, Zhang Q, Yu JR - Cancer Sci. (2014)

Characterization and quantification of blood plasmacytoid dendritic cells (pDCs). (a) PBMCs isolated from a gastric cancer (GC) patient and a control subject were analyzed for pDC levels by flow cytometry. The first gate was set on lymphocytes and monocytes (P1) and the second gate was set on Lineage− and HLA-DR+ cells (Q1). The pDCs were identified as Lineage−HLA-DR+CD123brightCD11c− cells. (b) Cumulative percentages of pDCs in Lineage− cells of the GC patient and the control subject. The prevalence of pDCs was significantly higher in the GC patient than that in the control donor. (c) The presence of pDCs was significantly associated with an increased number of ICOS+ regulatory T cells (Tregs) in peripheral blood. FSC, forward scatter; SSC, side scatter.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4317822&req=5

fig03: Characterization and quantification of blood plasmacytoid dendritic cells (pDCs). (a) PBMCs isolated from a gastric cancer (GC) patient and a control subject were analyzed for pDC levels by flow cytometry. The first gate was set on lymphocytes and monocytes (P1) and the second gate was set on Lineage− and HLA-DR+ cells (Q1). The pDCs were identified as Lineage−HLA-DR+CD123brightCD11c− cells. (b) Cumulative percentages of pDCs in Lineage− cells of the GC patient and the control subject. The prevalence of pDCs was significantly higher in the GC patient than that in the control donor. (c) The presence of pDCs was significantly associated with an increased number of ICOS+ regulatory T cells (Tregs) in peripheral blood. FSC, forward scatter; SSC, side scatter.
Mentions: Plasmacytoid dendritic cells were identified as Lin−HLA-DR+CD11c−CD123high cells (Fig. 3a). The proportion of circulating pDCs among Lineage− cells was significantly higher in GC patients than in control donors (2.64% [range, 0.14–10.70%] vs 1.51% [range, 0.11–6.91%], P = 0.027; Fig. 3b).

Bottom Line: We found that the numbers of pDCs, Tregs, and ICOS(+) Tregs in peripheral blood were increased in GC patients compared with healthy donors.In tissue, Tregs and ICOS(+) Tregs were found distributing mainly in carcinoma tissue, whereas pDCs were mainly found in peritumor tissue.In conclusion, pDCs may play a potential role in recruiting ICOS(+) Tregs, and both participate in the immunosuppression microenvironment of GC.

View Article: PubMed Central - PubMed

Affiliation: Department of Gastrointestinal Surgery, The First Affiliated Hospital, Medical College, Zhejiang University, Hangzhou, China.

Show MeSH
Related in: MedlinePlus