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Role of plasmacytoid dendritic cells and inducible costimulator-positive regulatory T cells in the immunosuppression microenvironment of gastric cancer.

Huang XM, Liu XS, Lin XK, Yu H, Sun JY, Liu XK, Chen C, Jin HL, Zhang GE, Shi XX, Zhang Q, Yu JR - Cancer Sci. (2014)

Bottom Line: We found that the numbers of pDCs, Tregs, and ICOS(+) Tregs in peripheral blood were increased in GC patients compared with healthy donors.In tissue, Tregs and ICOS(+) Tregs were found distributing mainly in carcinoma tissue, whereas pDCs were mainly found in peritumor tissue.In conclusion, pDCs may play a potential role in recruiting ICOS(+) Tregs, and both participate in the immunosuppression microenvironment of GC.

View Article: PubMed Central - PubMed

Affiliation: Department of Gastrointestinal Surgery, The First Affiliated Hospital, Medical College, Zhejiang University, Hangzhou, China.

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Related in: MedlinePlus

Increased population of regulatory T cells (Tregs) and inducible costimulator (ICOS)+ Tregs in carcinoma tissue. (a) Representative immunohistochemical (IHC) staining of Foxp3 in tumor, peritumor, and normal gastric tissue are shown (magnification, ×400). (b) Different tissues were stained for Foxp3 and ICOS using standard immunofluorescence techniques. FoxP3+ staining (green) was in the nucleus and ICOS+ staining (red) was on the surface of the cell. The top three photographs are shown at ×400 magnification, the three photographs below are shown at ×1000 magnification (green arrow, Foxp3+; red arrow, ICOS+; yellow arrow, double positive). (c) Box blot set showing the Foxp3+ cell level in different tissues (IHC). (d) ICOS+ Treg level was significantly higher in carcinoma tissue than in peritumor tissue or normal tissue. (e) Greater ICOS+ Treg/total Treg ratio is both in intratumor and peritumor tissue.
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fig02: Increased population of regulatory T cells (Tregs) and inducible costimulator (ICOS)+ Tregs in carcinoma tissue. (a) Representative immunohistochemical (IHC) staining of Foxp3 in tumor, peritumor, and normal gastric tissue are shown (magnification, ×400). (b) Different tissues were stained for Foxp3 and ICOS using standard immunofluorescence techniques. FoxP3+ staining (green) was in the nucleus and ICOS+ staining (red) was on the surface of the cell. The top three photographs are shown at ×400 magnification, the three photographs below are shown at ×1000 magnification (green arrow, Foxp3+; red arrow, ICOS+; yellow arrow, double positive). (c) Box blot set showing the Foxp3+ cell level in different tissues (IHC). (d) ICOS+ Treg level was significantly higher in carcinoma tissue than in peritumor tissue or normal tissue. (e) Greater ICOS+ Treg/total Treg ratio is both in intratumor and peritumor tissue.

Mentions: Circulating Tregs are defined as the population of CD4+CD25+Foxp3+ T cells as a percentage of CD4+ lymphocytes. There was an increased number of circulating Tregs in GC patients compared to the control group (4.69 ± 1.72% vs 3.38 ± 1.04%, P = 0.001; Fig. 1a,b). Moreover, the number of Foxp3+ T cells in tumor tissue (10.45 [range, 0.20–59.50]) are twice that in peritumor tissue (4.55 [range, 0.20–19.60], P = 0.001), and three times that in normal gastric mucosa (3.20 [range, 0.00–13.50], P = 0.001) (Fig. 2a,c). These results collectively suggest high enrichment of Tregs in GC patients.


Role of plasmacytoid dendritic cells and inducible costimulator-positive regulatory T cells in the immunosuppression microenvironment of gastric cancer.

Huang XM, Liu XS, Lin XK, Yu H, Sun JY, Liu XK, Chen C, Jin HL, Zhang GE, Shi XX, Zhang Q, Yu JR - Cancer Sci. (2014)

Increased population of regulatory T cells (Tregs) and inducible costimulator (ICOS)+ Tregs in carcinoma tissue. (a) Representative immunohistochemical (IHC) staining of Foxp3 in tumor, peritumor, and normal gastric tissue are shown (magnification, ×400). (b) Different tissues were stained for Foxp3 and ICOS using standard immunofluorescence techniques. FoxP3+ staining (green) was in the nucleus and ICOS+ staining (red) was on the surface of the cell. The top three photographs are shown at ×400 magnification, the three photographs below are shown at ×1000 magnification (green arrow, Foxp3+; red arrow, ICOS+; yellow arrow, double positive). (c) Box blot set showing the Foxp3+ cell level in different tissues (IHC). (d) ICOS+ Treg level was significantly higher in carcinoma tissue than in peritumor tissue or normal tissue. (e) Greater ICOS+ Treg/total Treg ratio is both in intratumor and peritumor tissue.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4317822&req=5

fig02: Increased population of regulatory T cells (Tregs) and inducible costimulator (ICOS)+ Tregs in carcinoma tissue. (a) Representative immunohistochemical (IHC) staining of Foxp3 in tumor, peritumor, and normal gastric tissue are shown (magnification, ×400). (b) Different tissues were stained for Foxp3 and ICOS using standard immunofluorescence techniques. FoxP3+ staining (green) was in the nucleus and ICOS+ staining (red) was on the surface of the cell. The top three photographs are shown at ×400 magnification, the three photographs below are shown at ×1000 magnification (green arrow, Foxp3+; red arrow, ICOS+; yellow arrow, double positive). (c) Box blot set showing the Foxp3+ cell level in different tissues (IHC). (d) ICOS+ Treg level was significantly higher in carcinoma tissue than in peritumor tissue or normal tissue. (e) Greater ICOS+ Treg/total Treg ratio is both in intratumor and peritumor tissue.
Mentions: Circulating Tregs are defined as the population of CD4+CD25+Foxp3+ T cells as a percentage of CD4+ lymphocytes. There was an increased number of circulating Tregs in GC patients compared to the control group (4.69 ± 1.72% vs 3.38 ± 1.04%, P = 0.001; Fig. 1a,b). Moreover, the number of Foxp3+ T cells in tumor tissue (10.45 [range, 0.20–59.50]) are twice that in peritumor tissue (4.55 [range, 0.20–19.60], P = 0.001), and three times that in normal gastric mucosa (3.20 [range, 0.00–13.50], P = 0.001) (Fig. 2a,c). These results collectively suggest high enrichment of Tregs in GC patients.

Bottom Line: We found that the numbers of pDCs, Tregs, and ICOS(+) Tregs in peripheral blood were increased in GC patients compared with healthy donors.In tissue, Tregs and ICOS(+) Tregs were found distributing mainly in carcinoma tissue, whereas pDCs were mainly found in peritumor tissue.In conclusion, pDCs may play a potential role in recruiting ICOS(+) Tregs, and both participate in the immunosuppression microenvironment of GC.

View Article: PubMed Central - PubMed

Affiliation: Department of Gastrointestinal Surgery, The First Affiliated Hospital, Medical College, Zhejiang University, Hangzhou, China.

Show MeSH
Related in: MedlinePlus