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Role of plasmacytoid dendritic cells and inducible costimulator-positive regulatory T cells in the immunosuppression microenvironment of gastric cancer.

Huang XM, Liu XS, Lin XK, Yu H, Sun JY, Liu XK, Chen C, Jin HL, Zhang GE, Shi XX, Zhang Q, Yu JR - Cancer Sci. (2014)

Bottom Line: We found that the numbers of pDCs, Tregs, and ICOS(+) Tregs in peripheral blood were increased in GC patients compared with healthy donors.In tissue, Tregs and ICOS(+) Tregs were found distributing mainly in carcinoma tissue, whereas pDCs were mainly found in peritumor tissue.In conclusion, pDCs may play a potential role in recruiting ICOS(+) Tregs, and both participate in the immunosuppression microenvironment of GC.

View Article: PubMed Central - PubMed

Affiliation: Department of Gastrointestinal Surgery, The First Affiliated Hospital, Medical College, Zhejiang University, Hangzhou, China.

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Increased numbers of regulatory T cells (Tregs) and inducible costimulator (ICOS)+ Tregs in peripheral blood of gastric cancer (GC) patients. (a) Flow cytometric CD4-gated analysis of CD25+Foxp3+ cells was carried out in a GC patient and a control. (b) The percentage of Tregs among CD4+ lymphocytes was higher in the GC patient than that in the control. (c) PBMCs were stained with anti-human ICOS PE-Cy7 and its isotype. (d) The proportion of ICOS+ Tregs and ICOS− Tregs among CD4+ lymphocytes was statistically increased in the GC group compared to the control group.
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fig01: Increased numbers of regulatory T cells (Tregs) and inducible costimulator (ICOS)+ Tregs in peripheral blood of gastric cancer (GC) patients. (a) Flow cytometric CD4-gated analysis of CD25+Foxp3+ cells was carried out in a GC patient and a control. (b) The percentage of Tregs among CD4+ lymphocytes was higher in the GC patient than that in the control. (c) PBMCs were stained with anti-human ICOS PE-Cy7 and its isotype. (d) The proportion of ICOS+ Tregs and ICOS− Tregs among CD4+ lymphocytes was statistically increased in the GC group compared to the control group.

Mentions: Circulating Tregs are defined as the population of CD4+CD25+Foxp3+ T cells as a percentage of CD4+ lymphocytes. There was an increased number of circulating Tregs in GC patients compared to the control group (4.69 ± 1.72% vs 3.38 ± 1.04%, P = 0.001; Fig. 1a,b). Moreover, the number of Foxp3+ T cells in tumor tissue (10.45 [range, 0.20–59.50]) are twice that in peritumor tissue (4.55 [range, 0.20–19.60], P = 0.001), and three times that in normal gastric mucosa (3.20 [range, 0.00–13.50], P = 0.001) (Fig. 2a,c). These results collectively suggest high enrichment of Tregs in GC patients.


Role of plasmacytoid dendritic cells and inducible costimulator-positive regulatory T cells in the immunosuppression microenvironment of gastric cancer.

Huang XM, Liu XS, Lin XK, Yu H, Sun JY, Liu XK, Chen C, Jin HL, Zhang GE, Shi XX, Zhang Q, Yu JR - Cancer Sci. (2014)

Increased numbers of regulatory T cells (Tregs) and inducible costimulator (ICOS)+ Tregs in peripheral blood of gastric cancer (GC) patients. (a) Flow cytometric CD4-gated analysis of CD25+Foxp3+ cells was carried out in a GC patient and a control. (b) The percentage of Tregs among CD4+ lymphocytes was higher in the GC patient than that in the control. (c) PBMCs were stained with anti-human ICOS PE-Cy7 and its isotype. (d) The proportion of ICOS+ Tregs and ICOS− Tregs among CD4+ lymphocytes was statistically increased in the GC group compared to the control group.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4317822&req=5

fig01: Increased numbers of regulatory T cells (Tregs) and inducible costimulator (ICOS)+ Tregs in peripheral blood of gastric cancer (GC) patients. (a) Flow cytometric CD4-gated analysis of CD25+Foxp3+ cells was carried out in a GC patient and a control. (b) The percentage of Tregs among CD4+ lymphocytes was higher in the GC patient than that in the control. (c) PBMCs were stained with anti-human ICOS PE-Cy7 and its isotype. (d) The proportion of ICOS+ Tregs and ICOS− Tregs among CD4+ lymphocytes was statistically increased in the GC group compared to the control group.
Mentions: Circulating Tregs are defined as the population of CD4+CD25+Foxp3+ T cells as a percentage of CD4+ lymphocytes. There was an increased number of circulating Tregs in GC patients compared to the control group (4.69 ± 1.72% vs 3.38 ± 1.04%, P = 0.001; Fig. 1a,b). Moreover, the number of Foxp3+ T cells in tumor tissue (10.45 [range, 0.20–59.50]) are twice that in peritumor tissue (4.55 [range, 0.20–19.60], P = 0.001), and three times that in normal gastric mucosa (3.20 [range, 0.00–13.50], P = 0.001) (Fig. 2a,c). These results collectively suggest high enrichment of Tregs in GC patients.

Bottom Line: We found that the numbers of pDCs, Tregs, and ICOS(+) Tregs in peripheral blood were increased in GC patients compared with healthy donors.In tissue, Tregs and ICOS(+) Tregs were found distributing mainly in carcinoma tissue, whereas pDCs were mainly found in peritumor tissue.In conclusion, pDCs may play a potential role in recruiting ICOS(+) Tregs, and both participate in the immunosuppression microenvironment of GC.

View Article: PubMed Central - PubMed

Affiliation: Department of Gastrointestinal Surgery, The First Affiliated Hospital, Medical College, Zhejiang University, Hangzhou, China.

Show MeSH
Related in: MedlinePlus