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Macrophage inhibitory factor 1 acts as a potential biomarker in patients with esophageal squamous cell carcinoma and is a target for antibody-based therapy.

Wang XB, Jiang XR, Yu XY, Wang L, He S, Feng FY, Guo LP, Jiang W, Lu SH - Cancer Sci. (2014)

Bottom Line: The results showed that the serum MIC1 of ESCC was significantly higher than normal groups (P < 0.001), and was positively associated with tumor invasion (P = 0.030) as well as lymph node metastasis (P = 0.007).The antibody of MIC1 inhibited the tumor growth (P < 0.001), and showing preference for tumor tissues in xenograft model.The decreased formation of neovascularization lumen may be involved in the mechanism.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Molecular Oncology, Department of Etiology and Carcinogenesis, Cancer Institute and Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

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Expression macrophage inhibitory factor 1 (MIC1) in cell lines and tissue samples. (a) Western blotting analysis of MIC1 expression in eight established esophageal squamous cell carcinoma (ESCC) cell lines and one human normal esophageal cell line. (b) The level of MIC1 in cell culture media detected by enzyme linked immunosorbent assay (ELISA). (c) MIC1 expression detected by IHC staining in tissue microarrays (TMA) of ESCC and normal tissues. Overview of the TMA (up) and two representative cylinders (down; i), Negative expression of MIC1 was shown in Normal esophageal tissue (ii). Weak positive (iii) and strong (iv) expression of MIC1 in ESCC tissues. (d) MIC expression in paired ESCC cancerous (T) and surrounding normal tissues (N), normalized by GAPDH (glyceraldehyde 3-phosphate dehydrogenase). (e) Western blotting analysis of MIC1 protein expression. (f) Relative expression of MIC1 in tissues grouped by total tumor node metastasis stage, normalized by GAPDH. (g) Relative expression of MIC1 in ESCC tissues classified according to N stage.
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fig03: Expression macrophage inhibitory factor 1 (MIC1) in cell lines and tissue samples. (a) Western blotting analysis of MIC1 expression in eight established esophageal squamous cell carcinoma (ESCC) cell lines and one human normal esophageal cell line. (b) The level of MIC1 in cell culture media detected by enzyme linked immunosorbent assay (ELISA). (c) MIC1 expression detected by IHC staining in tissue microarrays (TMA) of ESCC and normal tissues. Overview of the TMA (up) and two representative cylinders (down; i), Negative expression of MIC1 was shown in Normal esophageal tissue (ii). Weak positive (iii) and strong (iv) expression of MIC1 in ESCC tissues. (d) MIC expression in paired ESCC cancerous (T) and surrounding normal tissues (N), normalized by GAPDH (glyceraldehyde 3-phosphate dehydrogenase). (e) Western blotting analysis of MIC1 protein expression. (f) Relative expression of MIC1 in tissues grouped by total tumor node metastasis stage, normalized by GAPDH. (g) Relative expression of MIC1 in ESCC tissues classified according to N stage.

Mentions: To evaluate the role of MIC1 in ESCC progression, we first investigated the expression of MIC1 in ESCC cell lines and tumor tissues. Elevated expression of MIC1 was observed in three (37.5%) ESCC cell lines as well as in culture medium compared with that in normal esophageal cell lines (Fig. 3a,b). Then MIC1 protein expression level was examined by ESCC tissue microarray immunohistochemical staining. We found that normal esophageal epithelia showed negative or weak immunoreactions, yet cancer tissues demonstrated weak to intense positive staining in 18 of 40 (45%) ESCC specimens and the positive staining of MIC1 in ESCC tissues were mostly confined to the cytoplasm of ESCC cells with a diffuse pattern (Fig. 3c).


Macrophage inhibitory factor 1 acts as a potential biomarker in patients with esophageal squamous cell carcinoma and is a target for antibody-based therapy.

Wang XB, Jiang XR, Yu XY, Wang L, He S, Feng FY, Guo LP, Jiang W, Lu SH - Cancer Sci. (2014)

Expression macrophage inhibitory factor 1 (MIC1) in cell lines and tissue samples. (a) Western blotting analysis of MIC1 expression in eight established esophageal squamous cell carcinoma (ESCC) cell lines and one human normal esophageal cell line. (b) The level of MIC1 in cell culture media detected by enzyme linked immunosorbent assay (ELISA). (c) MIC1 expression detected by IHC staining in tissue microarrays (TMA) of ESCC and normal tissues. Overview of the TMA (up) and two representative cylinders (down; i), Negative expression of MIC1 was shown in Normal esophageal tissue (ii). Weak positive (iii) and strong (iv) expression of MIC1 in ESCC tissues. (d) MIC expression in paired ESCC cancerous (T) and surrounding normal tissues (N), normalized by GAPDH (glyceraldehyde 3-phosphate dehydrogenase). (e) Western blotting analysis of MIC1 protein expression. (f) Relative expression of MIC1 in tissues grouped by total tumor node metastasis stage, normalized by GAPDH. (g) Relative expression of MIC1 in ESCC tissues classified according to N stage.
© Copyright Policy - open-access
Related In: Results  -  Collection

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fig03: Expression macrophage inhibitory factor 1 (MIC1) in cell lines and tissue samples. (a) Western blotting analysis of MIC1 expression in eight established esophageal squamous cell carcinoma (ESCC) cell lines and one human normal esophageal cell line. (b) The level of MIC1 in cell culture media detected by enzyme linked immunosorbent assay (ELISA). (c) MIC1 expression detected by IHC staining in tissue microarrays (TMA) of ESCC and normal tissues. Overview of the TMA (up) and two representative cylinders (down; i), Negative expression of MIC1 was shown in Normal esophageal tissue (ii). Weak positive (iii) and strong (iv) expression of MIC1 in ESCC tissues. (d) MIC expression in paired ESCC cancerous (T) and surrounding normal tissues (N), normalized by GAPDH (glyceraldehyde 3-phosphate dehydrogenase). (e) Western blotting analysis of MIC1 protein expression. (f) Relative expression of MIC1 in tissues grouped by total tumor node metastasis stage, normalized by GAPDH. (g) Relative expression of MIC1 in ESCC tissues classified according to N stage.
Mentions: To evaluate the role of MIC1 in ESCC progression, we first investigated the expression of MIC1 in ESCC cell lines and tumor tissues. Elevated expression of MIC1 was observed in three (37.5%) ESCC cell lines as well as in culture medium compared with that in normal esophageal cell lines (Fig. 3a,b). Then MIC1 protein expression level was examined by ESCC tissue microarray immunohistochemical staining. We found that normal esophageal epithelia showed negative or weak immunoreactions, yet cancer tissues demonstrated weak to intense positive staining in 18 of 40 (45%) ESCC specimens and the positive staining of MIC1 in ESCC tissues were mostly confined to the cytoplasm of ESCC cells with a diffuse pattern (Fig. 3c).

Bottom Line: The results showed that the serum MIC1 of ESCC was significantly higher than normal groups (P < 0.001), and was positively associated with tumor invasion (P = 0.030) as well as lymph node metastasis (P = 0.007).The antibody of MIC1 inhibited the tumor growth (P < 0.001), and showing preference for tumor tissues in xenograft model.The decreased formation of neovascularization lumen may be involved in the mechanism.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Molecular Oncology, Department of Etiology and Carcinogenesis, Cancer Institute and Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

Show MeSH
Related in: MedlinePlus