MicroRNA-143 regulates collagen type III expression in stromal fibroblasts of scirrhous type gastric cancer.
Bottom Line: In stromal cells, miR-143 enhanced collagen type III expression in normal gastric fibroblasts and cancer-associated fibroblasts through activation of transforming growth factor-β)/SMAD signaling.Furthermore, high miR-143 expression in GC was associated with worse cancer-specific mortality (P = 0.0141).These data suggest that miR-143 regulates fibrosis of scirrhous type GC through induction of collagen expression in stromal fibroblasts and that miR-143 expression serves as a prognostic marker of GC.
Affiliation: Department of Molecular Pathology, Institute of Biomedical and Health Sciences, Hiroshima University, Hiroshima, Japan.Show MeSH
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Mentions: To investigate the relation between miR-143 expression and clinicopathological parameters, we examined miR-143 expression levels in 68 formalin-fixed paraffin-embedded samples of primary GC by qRT-PCR. The miR-143 expression levels were significantly higher in scirrhous type GC cases than in non-scirrhous type GC cases (P = 0.0167; Fig. 5a). As shown in Table 2, miR-143-positive GC cases showed advanced tumor stage (P = 0.0013) and tended to have scirrhous type histology (P = 0.0428) in comparison with miR-143-negative cases. Kaplan–Meier analysis showed that GC patients with high miR-143 expression correlated significantly with worse cancer-specific mortality (P = 0.0002, log–rank test; Fig. 5b). When limited to the cases with scirrhous type GC or non-scirrhous type GC, miR-143 expression had no prognostic effect (data not shown). Univariate and multivariate analysis showed miR-143 to be an independent prognostic marker of GC cases (P = 0.0141; Table 3).
Affiliation: Department of Molecular Pathology, Institute of Biomedical and Health Sciences, Hiroshima University, Hiroshima, Japan.