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Context-dependent activation of Wnt signaling by tumor suppressor RUNX3 in gastric cancer cells.

Ju X, Ishikawa TO, Naka K, Ito K, Ito Y, Oshima M - Cancer Sci. (2014)

Bottom Line: Here, we confirmed that RUNX3 suppressed Wnt signaling activity in several gastric cancer cell lines; however, we found that RUNX3 increased the Wnt signaling activity in KatoIII and SNU668 gastric cancer cells.As found previously, RUNX3 also binds to TCF4 and β-catenin in KatoIII cells, suggesting that these molecules form a ternary complex.These results indicate that RUNX3 can either suppress or activate the Wnt signaling pathway through its binding to the TCF4/β-catenin complex by cell context-dependent mechanisms.

View Article: PubMed Central - PubMed

Affiliation: Division of Genetics, Cancer Research Institute, Kanazawa University, Kanazawa, Japan.

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Suppression of tumorigenicity of KatoIII cells by RUNX3. (a) Relative cell proliferation rates of RUNX3 vector-transfected KatoIII cells and control vector-transfected cells at the indicated time points (mean ± SD). *P < 0.05. (b) Flow cytometry analyses for GFP expression in the control (left) and RUNX3-IRES-mGFP-transfected (right) KatoIII cells. (c) Representative photographs of GFPHi (left) and GFPLo (right) cells sorted from RUNX3-IRES-mGFP-transfected KatoIII cells after culture for 6 days. (d) Representative photographs of the soft agar colonies of the control (left) and RUNX3-transfected KatoIII cells (right). (e) The number of soft agar colonies per dissecting microscopic field. *P < 0.05. (f) Relative p21 mRNA expression level in RUNX3 vector-transfected KatoIII cells to the control cells (mean ± SD). *P < 0.05.
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fig04: Suppression of tumorigenicity of KatoIII cells by RUNX3. (a) Relative cell proliferation rates of RUNX3 vector-transfected KatoIII cells and control vector-transfected cells at the indicated time points (mean ± SD). *P < 0.05. (b) Flow cytometry analyses for GFP expression in the control (left) and RUNX3-IRES-mGFP-transfected (right) KatoIII cells. (c) Representative photographs of GFPHi (left) and GFPLo (right) cells sorted from RUNX3-IRES-mGFP-transfected KatoIII cells after culture for 6 days. (d) Representative photographs of the soft agar colonies of the control (left) and RUNX3-transfected KatoIII cells (right). (e) The number of soft agar colonies per dissecting microscopic field. *P < 0.05. (f) Relative p21 mRNA expression level in RUNX3 vector-transfected KatoIII cells to the control cells (mean ± SD). *P < 0.05.

Mentions: It has been reported that RUNX3 suppresses the tumorigenicity of KatoIII cells.(19) Therefore, we reexamined the role of RUNX3 in the tumorigenicity of KatoIII cells. The RUNX3 vector-transfected KatoIII cells exhibited slight but significant suppression of cell proliferation at 48 and 72 h after seeding (Fig. 4a), and RUNX3 siRNA increased proliferation KatoIII-R3 cells at 48 h after seeding (Fig. S5).


Context-dependent activation of Wnt signaling by tumor suppressor RUNX3 in gastric cancer cells.

Ju X, Ishikawa TO, Naka K, Ito K, Ito Y, Oshima M - Cancer Sci. (2014)

Suppression of tumorigenicity of KatoIII cells by RUNX3. (a) Relative cell proliferation rates of RUNX3 vector-transfected KatoIII cells and control vector-transfected cells at the indicated time points (mean ± SD). *P < 0.05. (b) Flow cytometry analyses for GFP expression in the control (left) and RUNX3-IRES-mGFP-transfected (right) KatoIII cells. (c) Representative photographs of GFPHi (left) and GFPLo (right) cells sorted from RUNX3-IRES-mGFP-transfected KatoIII cells after culture for 6 days. (d) Representative photographs of the soft agar colonies of the control (left) and RUNX3-transfected KatoIII cells (right). (e) The number of soft agar colonies per dissecting microscopic field. *P < 0.05. (f) Relative p21 mRNA expression level in RUNX3 vector-transfected KatoIII cells to the control cells (mean ± SD). *P < 0.05.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4317806&req=5

fig04: Suppression of tumorigenicity of KatoIII cells by RUNX3. (a) Relative cell proliferation rates of RUNX3 vector-transfected KatoIII cells and control vector-transfected cells at the indicated time points (mean ± SD). *P < 0.05. (b) Flow cytometry analyses for GFP expression in the control (left) and RUNX3-IRES-mGFP-transfected (right) KatoIII cells. (c) Representative photographs of GFPHi (left) and GFPLo (right) cells sorted from RUNX3-IRES-mGFP-transfected KatoIII cells after culture for 6 days. (d) Representative photographs of the soft agar colonies of the control (left) and RUNX3-transfected KatoIII cells (right). (e) The number of soft agar colonies per dissecting microscopic field. *P < 0.05. (f) Relative p21 mRNA expression level in RUNX3 vector-transfected KatoIII cells to the control cells (mean ± SD). *P < 0.05.
Mentions: It has been reported that RUNX3 suppresses the tumorigenicity of KatoIII cells.(19) Therefore, we reexamined the role of RUNX3 in the tumorigenicity of KatoIII cells. The RUNX3 vector-transfected KatoIII cells exhibited slight but significant suppression of cell proliferation at 48 and 72 h after seeding (Fig. 4a), and RUNX3 siRNA increased proliferation KatoIII-R3 cells at 48 h after seeding (Fig. S5).

Bottom Line: Here, we confirmed that RUNX3 suppressed Wnt signaling activity in several gastric cancer cell lines; however, we found that RUNX3 increased the Wnt signaling activity in KatoIII and SNU668 gastric cancer cells.As found previously, RUNX3 also binds to TCF4 and β-catenin in KatoIII cells, suggesting that these molecules form a ternary complex.These results indicate that RUNX3 can either suppress or activate the Wnt signaling pathway through its binding to the TCF4/β-catenin complex by cell context-dependent mechanisms.

View Article: PubMed Central - PubMed

Affiliation: Division of Genetics, Cancer Research Institute, Kanazawa University, Kanazawa, Japan.

Show MeSH
Related in: MedlinePlus