Context-dependent activation of Wnt signaling by tumor suppressor RUNX3 in gastric cancer cells.
Bottom Line: Here, we confirmed that RUNX3 suppressed Wnt signaling activity in several gastric cancer cell lines; however, we found that RUNX3 increased the Wnt signaling activity in KatoIII and SNU668 gastric cancer cells.As found previously, RUNX3 also binds to TCF4 and β-catenin in KatoIII cells, suggesting that these molecules form a ternary complex.These results indicate that RUNX3 can either suppress or activate the Wnt signaling pathway through its binding to the TCF4/β-catenin complex by cell context-dependent mechanisms.
Affiliation: Division of Genetics, Cancer Research Institute, Kanazawa University, Kanazawa, Japan.Show MeSH
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Mentions: It has been reported that RUNX3 suppresses the tumorigenicity of KatoIII cells.(19) Therefore, we reexamined the role of RUNX3 in the tumorigenicity of KatoIII cells. The RUNX3 vector-transfected KatoIII cells exhibited slight but significant suppression of cell proliferation at 48 and 72 h after seeding (Fig. 4a), and RUNX3 siRNA increased proliferation KatoIII-R3 cells at 48 h after seeding (Fig. S5).
Affiliation: Division of Genetics, Cancer Research Institute, Kanazawa University, Kanazawa, Japan.