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Effect of microRNA-210 on prognosis and response to chemotherapeutic drugs in pediatric acute lymphoblastic leukemia.

Mei Y, Gao C, Wang K, Cui L, Li W, Zhao X, Liu F, Wu M, Deng G, Ding W, Jia H, Li Z - Cancer Sci. (2014)

Bottom Line: We further examined its effect on the response to chemotherapeutic drugs in the Reh and RS4;11 cell lines.A significantly poorer treatment outcome (P < 0.05) was found in the low-expression group and verified in the validation cohort (76 cases, P < 0.05).Increasing/decreasing miR-210 expression using agomir/antagomir could enhance or reduce the response of Reh cells and RS4;11 cells to daunorubicin/dexamethasone/L-asparaginase and daunorubicin/dexamethasone/vincristine, respectively.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Major Diseases in Children (Capital Medical University), Ministry of Education, National Key Discipline of Pediatrics, Ministry of Education, Hematology Center, Beijing Children's Hospital, Capital Medical University, Beijing, China.

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Change in drug sensitivity of Reh cells determined by MTS assay. The cells were transfected with miR-210 agomir, antagomir and controls, respectively. The cell viability after treated with drug mixture at concentrations of IC5 (a,b), IC25 (c,d), IC50 (e,f) IC75 (g,h), and IC95 (i,j) were shown. Data are shown as means ± SE.
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fig06: Change in drug sensitivity of Reh cells determined by MTS assay. The cells were transfected with miR-210 agomir, antagomir and controls, respectively. The cell viability after treated with drug mixture at concentrations of IC5 (a,b), IC25 (c,d), IC50 (e,f) IC75 (g,h), and IC95 (i,j) were shown. Data are shown as means ± SE.

Mentions: As combinations of the four drugs were administered in chemotherapy, the effects of changing the intracellular level of miR-210 on the response of the two cell lines to multidrug treatment were examined. The two cell lines after transfection with miR-210 agomir or antagomir were treated with mixtures of the four drugs at five concentrations (IC5, IC25, IC50, IC75 and IC95). miR-210 agomir and antagomir significantly decreased or increased the viability of cells treated with the four drugs, respectively, at concentrations of IC5 through IC75 (Figs 6a–h, 7a–h) but had no effect on the viability of cells treated with the four drugs at a concentration of IC95 (Figs 6i–j, 7i–j). At high concentration, the drug combination probably overcomes the resistance attributable to low expression of miR-210.


Effect of microRNA-210 on prognosis and response to chemotherapeutic drugs in pediatric acute lymphoblastic leukemia.

Mei Y, Gao C, Wang K, Cui L, Li W, Zhao X, Liu F, Wu M, Deng G, Ding W, Jia H, Li Z - Cancer Sci. (2014)

Change in drug sensitivity of Reh cells determined by MTS assay. The cells were transfected with miR-210 agomir, antagomir and controls, respectively. The cell viability after treated with drug mixture at concentrations of IC5 (a,b), IC25 (c,d), IC50 (e,f) IC75 (g,h), and IC95 (i,j) were shown. Data are shown as means ± SE.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4317805&req=5

fig06: Change in drug sensitivity of Reh cells determined by MTS assay. The cells were transfected with miR-210 agomir, antagomir and controls, respectively. The cell viability after treated with drug mixture at concentrations of IC5 (a,b), IC25 (c,d), IC50 (e,f) IC75 (g,h), and IC95 (i,j) were shown. Data are shown as means ± SE.
Mentions: As combinations of the four drugs were administered in chemotherapy, the effects of changing the intracellular level of miR-210 on the response of the two cell lines to multidrug treatment were examined. The two cell lines after transfection with miR-210 agomir or antagomir were treated with mixtures of the four drugs at five concentrations (IC5, IC25, IC50, IC75 and IC95). miR-210 agomir and antagomir significantly decreased or increased the viability of cells treated with the four drugs, respectively, at concentrations of IC5 through IC75 (Figs 6a–h, 7a–h) but had no effect on the viability of cells treated with the four drugs at a concentration of IC95 (Figs 6i–j, 7i–j). At high concentration, the drug combination probably overcomes the resistance attributable to low expression of miR-210.

Bottom Line: We further examined its effect on the response to chemotherapeutic drugs in the Reh and RS4;11 cell lines.A significantly poorer treatment outcome (P < 0.05) was found in the low-expression group and verified in the validation cohort (76 cases, P < 0.05).Increasing/decreasing miR-210 expression using agomir/antagomir could enhance or reduce the response of Reh cells and RS4;11 cells to daunorubicin/dexamethasone/L-asparaginase and daunorubicin/dexamethasone/vincristine, respectively.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Major Diseases in Children (Capital Medical University), Ministry of Education, National Key Discipline of Pediatrics, Ministry of Education, Hematology Center, Beijing Children's Hospital, Capital Medical University, Beijing, China.

Show MeSH
Related in: MedlinePlus