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Effect of microRNA-210 on prognosis and response to chemotherapeutic drugs in pediatric acute lymphoblastic leukemia.

Mei Y, Gao C, Wang K, Cui L, Li W, Zhao X, Liu F, Wu M, Deng G, Ding W, Jia H, Li Z - Cancer Sci. (2014)

Bottom Line: We further examined its effect on the response to chemotherapeutic drugs in the Reh and RS4;11 cell lines.A significantly poorer treatment outcome (P < 0.05) was found in the low-expression group and verified in the validation cohort (76 cases, P < 0.05).Increasing/decreasing miR-210 expression using agomir/antagomir could enhance or reduce the response of Reh cells and RS4;11 cells to daunorubicin/dexamethasone/L-asparaginase and daunorubicin/dexamethasone/vincristine, respectively.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Major Diseases in Children (Capital Medical University), Ministry of Education, National Key Discipline of Pediatrics, Ministry of Education, Hematology Center, Beijing Children's Hospital, Capital Medical University, Beijing, China.

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Change in drug sensitivity of Reh cells determined by MTS assay. The cells were transfected with miR-210 agomir, antagomir and controls, respectively. The drug response to DNR (a,b), L-ASP (c, d), DEX (e, f) and VCR (g, h) are shown.
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fig04: Change in drug sensitivity of Reh cells determined by MTS assay. The cells were transfected with miR-210 agomir, antagomir and controls, respectively. The drug response to DNR (a,b), L-ASP (c, d), DEX (e, f) and VCR (g, h) are shown.

Mentions: In Reh cells transfected with miR-210 agomir or antagomir, the IC50s of DNR, L-ASP, and DEX were significantly decreased or increased compared with that in cells transfected with agomir-NC or antagomir-NC, respectively (Fig. 4a–f). However, miR-210 agomir and antagomir had no effect on the IC50 of VCR (Fig. 4g–h).


Effect of microRNA-210 on prognosis and response to chemotherapeutic drugs in pediatric acute lymphoblastic leukemia.

Mei Y, Gao C, Wang K, Cui L, Li W, Zhao X, Liu F, Wu M, Deng G, Ding W, Jia H, Li Z - Cancer Sci. (2014)

Change in drug sensitivity of Reh cells determined by MTS assay. The cells were transfected with miR-210 agomir, antagomir and controls, respectively. The drug response to DNR (a,b), L-ASP (c, d), DEX (e, f) and VCR (g, h) are shown.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4317805&req=5

fig04: Change in drug sensitivity of Reh cells determined by MTS assay. The cells were transfected with miR-210 agomir, antagomir and controls, respectively. The drug response to DNR (a,b), L-ASP (c, d), DEX (e, f) and VCR (g, h) are shown.
Mentions: In Reh cells transfected with miR-210 agomir or antagomir, the IC50s of DNR, L-ASP, and DEX were significantly decreased or increased compared with that in cells transfected with agomir-NC or antagomir-NC, respectively (Fig. 4a–f). However, miR-210 agomir and antagomir had no effect on the IC50 of VCR (Fig. 4g–h).

Bottom Line: We further examined its effect on the response to chemotherapeutic drugs in the Reh and RS4;11 cell lines.A significantly poorer treatment outcome (P < 0.05) was found in the low-expression group and verified in the validation cohort (76 cases, P < 0.05).Increasing/decreasing miR-210 expression using agomir/antagomir could enhance or reduce the response of Reh cells and RS4;11 cells to daunorubicin/dexamethasone/L-asparaginase and daunorubicin/dexamethasone/vincristine, respectively.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Major Diseases in Children (Capital Medical University), Ministry of Education, National Key Discipline of Pediatrics, Ministry of Education, Hematology Center, Beijing Children's Hospital, Capital Medical University, Beijing, China.

Show MeSH
Related in: MedlinePlus