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Sirtuin 1 facilitates chemoresistance of pancreatic cancer cells by regulating adaptive response to chemotherapy-induced stress.

Zhang JG, Hong DF, Zhang CW, Sun XD, Wang ZF, Shi Y, Liu JW, Shen GL, Zhang YB, Cheng J, Wang CY, Zhao G - Cancer Sci. (2014)

Bottom Line: In the present study, SIRT1 in PANC-1, BXPC-3, and ASPC-1 cells was upregulated after treatment with gemcitabine.Western blot results also showed that SIRT1, acetylated-p53, FOXO3a, and p21 were upregulated after combined treatment, whereas no obvious change was evident in total p53 protein.Thus, our results indicated a special role for SIRT1 in the regulation of adaptive response to chemotherapy-induced stress, which is involved in chemoresistance.

View Article: PubMed Central - PubMed

Affiliation: Hepatobiliary and Pancreatic Surgery, Zhejiang Provincial People's Hospital, Hangzhou, China; Pancreatic Disease Institute, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

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Induction of cellular senescence in pancreatic cancer cells treated with EX527 and (or) gemcitabine (GEM). PANC-1 (a) and ASPC-1 (b) cells were treated with EX527 (2 μM) and (or) GEM (50 μg/mL) for 48 h, and senescence-associated-β-gal activity (blue) was measured (×400). The graphs show the relationship between cellular senescence and each drug. Error bars represent mean ± SD. **P < 0.01 versus control group; #P < 0.01 versus single drug treatment.
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fig07: Induction of cellular senescence in pancreatic cancer cells treated with EX527 and (or) gemcitabine (GEM). PANC-1 (a) and ASPC-1 (b) cells were treated with EX527 (2 μM) and (or) GEM (50 μg/mL) for 48 h, and senescence-associated-β-gal activity (blue) was measured (×400). The graphs show the relationship between cellular senescence and each drug. Error bars represent mean ± SD. **P < 0.01 versus control group; #P < 0.01 versus single drug treatment.

Mentions: According to the results of the SA-β-gal assay, compared to untreated PANC-1 and ASPC-1 cells, SA-β-gal-positive cells were mildly increased in GEM-treated PANC-1 and ASPC-1 cells, and moderately increased in EX527-treated cells. Moreover, compared to GEM or EX527 alone, the combination of GEM and EX527 significantly increased the number of SA-β-gal-positive cells in PANC-1 and ASPC-1 cells even further (Fig. 7).


Sirtuin 1 facilitates chemoresistance of pancreatic cancer cells by regulating adaptive response to chemotherapy-induced stress.

Zhang JG, Hong DF, Zhang CW, Sun XD, Wang ZF, Shi Y, Liu JW, Shen GL, Zhang YB, Cheng J, Wang CY, Zhao G - Cancer Sci. (2014)

Induction of cellular senescence in pancreatic cancer cells treated with EX527 and (or) gemcitabine (GEM). PANC-1 (a) and ASPC-1 (b) cells were treated with EX527 (2 μM) and (or) GEM (50 μg/mL) for 48 h, and senescence-associated-β-gal activity (blue) was measured (×400). The graphs show the relationship between cellular senescence and each drug. Error bars represent mean ± SD. **P < 0.01 versus control group; #P < 0.01 versus single drug treatment.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4317803&req=5

fig07: Induction of cellular senescence in pancreatic cancer cells treated with EX527 and (or) gemcitabine (GEM). PANC-1 (a) and ASPC-1 (b) cells were treated with EX527 (2 μM) and (or) GEM (50 μg/mL) for 48 h, and senescence-associated-β-gal activity (blue) was measured (×400). The graphs show the relationship between cellular senescence and each drug. Error bars represent mean ± SD. **P < 0.01 versus control group; #P < 0.01 versus single drug treatment.
Mentions: According to the results of the SA-β-gal assay, compared to untreated PANC-1 and ASPC-1 cells, SA-β-gal-positive cells were mildly increased in GEM-treated PANC-1 and ASPC-1 cells, and moderately increased in EX527-treated cells. Moreover, compared to GEM or EX527 alone, the combination of GEM and EX527 significantly increased the number of SA-β-gal-positive cells in PANC-1 and ASPC-1 cells even further (Fig. 7).

Bottom Line: In the present study, SIRT1 in PANC-1, BXPC-3, and ASPC-1 cells was upregulated after treatment with gemcitabine.Western blot results also showed that SIRT1, acetylated-p53, FOXO3a, and p21 were upregulated after combined treatment, whereas no obvious change was evident in total p53 protein.Thus, our results indicated a special role for SIRT1 in the regulation of adaptive response to chemotherapy-induced stress, which is involved in chemoresistance.

View Article: PubMed Central - PubMed

Affiliation: Hepatobiliary and Pancreatic Surgery, Zhejiang Provincial People's Hospital, Hangzhou, China; Pancreatic Disease Institute, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Show MeSH
Related in: MedlinePlus