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Sirtuin 1 facilitates chemoresistance of pancreatic cancer cells by regulating adaptive response to chemotherapy-induced stress.

Zhang JG, Hong DF, Zhang CW, Sun XD, Wang ZF, Shi Y, Liu JW, Shen GL, Zhang YB, Cheng J, Wang CY, Zhao G - Cancer Sci. (2014)

Bottom Line: In the present study, SIRT1 in PANC-1, BXPC-3, and ASPC-1 cells was upregulated after treatment with gemcitabine.Western blot results also showed that SIRT1, acetylated-p53, FOXO3a, and p21 were upregulated after combined treatment, whereas no obvious change was evident in total p53 protein.Thus, our results indicated a special role for SIRT1 in the regulation of adaptive response to chemotherapy-induced stress, which is involved in chemoresistance.

View Article: PubMed Central - PubMed

Affiliation: Hepatobiliary and Pancreatic Surgery, Zhejiang Provincial People's Hospital, Hangzhou, China; Pancreatic Disease Institute, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

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Effect of EX527 and (or) gemcitabine (GEM) on cell cycle distribution in pancreatic cancer cells. Cycle phase distributions of PANC-1 (a) and ASPC-1 (b) cells were analyzed by flow cytometry after staining with propidium iodide. Percentage of cells in each phase of the cell cycle (G0/G1, G2/M and S-phase) is indicated. The experiments were repeated twice with similar results.
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fig06: Effect of EX527 and (or) gemcitabine (GEM) on cell cycle distribution in pancreatic cancer cells. Cycle phase distributions of PANC-1 (a) and ASPC-1 (b) cells were analyzed by flow cytometry after staining with propidium iodide. Percentage of cells in each phase of the cell cycle (G0/G1, G2/M and S-phase) is indicated. The experiments were repeated twice with similar results.

Mentions: When treated with GEM or EX527, the distribution of PANC-1 cells in G0/G1 phase notably increased (57.85 ± 2.29% vs 66.89 ± 1.99%, 76.79 ± 2.01%, P < 0.01; Fig. 6a). Moreover, compared to EX527 or GEM alone, treatment with a combination of both resulted in further significant increase of cells in G0/G1 phase (84.39 ± 1.30%, P < 0.01). These results were also confirmed in ASPC-1 cells (Fig. 6b).


Sirtuin 1 facilitates chemoresistance of pancreatic cancer cells by regulating adaptive response to chemotherapy-induced stress.

Zhang JG, Hong DF, Zhang CW, Sun XD, Wang ZF, Shi Y, Liu JW, Shen GL, Zhang YB, Cheng J, Wang CY, Zhao G - Cancer Sci. (2014)

Effect of EX527 and (or) gemcitabine (GEM) on cell cycle distribution in pancreatic cancer cells. Cycle phase distributions of PANC-1 (a) and ASPC-1 (b) cells were analyzed by flow cytometry after staining with propidium iodide. Percentage of cells in each phase of the cell cycle (G0/G1, G2/M and S-phase) is indicated. The experiments were repeated twice with similar results.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4317803&req=5

fig06: Effect of EX527 and (or) gemcitabine (GEM) on cell cycle distribution in pancreatic cancer cells. Cycle phase distributions of PANC-1 (a) and ASPC-1 (b) cells were analyzed by flow cytometry after staining with propidium iodide. Percentage of cells in each phase of the cell cycle (G0/G1, G2/M and S-phase) is indicated. The experiments were repeated twice with similar results.
Mentions: When treated with GEM or EX527, the distribution of PANC-1 cells in G0/G1 phase notably increased (57.85 ± 2.29% vs 66.89 ± 1.99%, 76.79 ± 2.01%, P < 0.01; Fig. 6a). Moreover, compared to EX527 or GEM alone, treatment with a combination of both resulted in further significant increase of cells in G0/G1 phase (84.39 ± 1.30%, P < 0.01). These results were also confirmed in ASPC-1 cells (Fig. 6b).

Bottom Line: In the present study, SIRT1 in PANC-1, BXPC-3, and ASPC-1 cells was upregulated after treatment with gemcitabine.Western blot results also showed that SIRT1, acetylated-p53, FOXO3a, and p21 were upregulated after combined treatment, whereas no obvious change was evident in total p53 protein.Thus, our results indicated a special role for SIRT1 in the regulation of adaptive response to chemotherapy-induced stress, which is involved in chemoresistance.

View Article: PubMed Central - PubMed

Affiliation: Hepatobiliary and Pancreatic Surgery, Zhejiang Provincial People's Hospital, Hangzhou, China; Pancreatic Disease Institute, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Show MeSH
Related in: MedlinePlus