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Sirtuin 1 facilitates chemoresistance of pancreatic cancer cells by regulating adaptive response to chemotherapy-induced stress.

Zhang JG, Hong DF, Zhang CW, Sun XD, Wang ZF, Shi Y, Liu JW, Shen GL, Zhang YB, Cheng J, Wang CY, Zhao G - Cancer Sci. (2014)

Bottom Line: In the present study, SIRT1 in PANC-1, BXPC-3, and ASPC-1 cells was upregulated after treatment with gemcitabine.Western blot results also showed that SIRT1, acetylated-p53, FOXO3a, and p21 were upregulated after combined treatment, whereas no obvious change was evident in total p53 protein.Thus, our results indicated a special role for SIRT1 in the regulation of adaptive response to chemotherapy-induced stress, which is involved in chemoresistance.

View Article: PubMed Central - PubMed

Affiliation: Hepatobiliary and Pancreatic Surgery, Zhejiang Provincial People's Hospital, Hangzhou, China; Pancreatic Disease Institute, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

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Induction of sirtuin 1 (SIRT1) in pancreatic cancer cell lines treated with gemcitabine (GEM). PANC-1, BXPC-3, and ASPC-1 cells were incubated with GEM (0, 5, 25 μg/mL) for 48 h. SIRT1 expression was monitored by Western blot analysis (a) and quantitative RT-PCR (b). The experiments were repeated twice and a representative result is shown. *P < 0.05, **P < 0.01 versus control group.
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fig01: Induction of sirtuin 1 (SIRT1) in pancreatic cancer cell lines treated with gemcitabine (GEM). PANC-1, BXPC-3, and ASPC-1 cells were incubated with GEM (0, 5, 25 μg/mL) for 48 h. SIRT1 expression was monitored by Western blot analysis (a) and quantitative RT-PCR (b). The experiments were repeated twice and a representative result is shown. *P < 0.05, **P < 0.01 versus control group.

Mentions: PANC-1, BXPC-3, and ASPC-1 cell lines were treated with GEM (0, 5, 25 μg/mL). The Western blot results showed that SIRT1 expression in those three cell lines were all significantly increased, and the same results were also found by qRT-PCR in RNA levels (Fig. 1).


Sirtuin 1 facilitates chemoresistance of pancreatic cancer cells by regulating adaptive response to chemotherapy-induced stress.

Zhang JG, Hong DF, Zhang CW, Sun XD, Wang ZF, Shi Y, Liu JW, Shen GL, Zhang YB, Cheng J, Wang CY, Zhao G - Cancer Sci. (2014)

Induction of sirtuin 1 (SIRT1) in pancreatic cancer cell lines treated with gemcitabine (GEM). PANC-1, BXPC-3, and ASPC-1 cells were incubated with GEM (0, 5, 25 μg/mL) for 48 h. SIRT1 expression was monitored by Western blot analysis (a) and quantitative RT-PCR (b). The experiments were repeated twice and a representative result is shown. *P < 0.05, **P < 0.01 versus control group.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4317803&req=5

fig01: Induction of sirtuin 1 (SIRT1) in pancreatic cancer cell lines treated with gemcitabine (GEM). PANC-1, BXPC-3, and ASPC-1 cells were incubated with GEM (0, 5, 25 μg/mL) for 48 h. SIRT1 expression was monitored by Western blot analysis (a) and quantitative RT-PCR (b). The experiments were repeated twice and a representative result is shown. *P < 0.05, **P < 0.01 versus control group.
Mentions: PANC-1, BXPC-3, and ASPC-1 cell lines were treated with GEM (0, 5, 25 μg/mL). The Western blot results showed that SIRT1 expression in those three cell lines were all significantly increased, and the same results were also found by qRT-PCR in RNA levels (Fig. 1).

Bottom Line: In the present study, SIRT1 in PANC-1, BXPC-3, and ASPC-1 cells was upregulated after treatment with gemcitabine.Western blot results also showed that SIRT1, acetylated-p53, FOXO3a, and p21 were upregulated after combined treatment, whereas no obvious change was evident in total p53 protein.Thus, our results indicated a special role for SIRT1 in the regulation of adaptive response to chemotherapy-induced stress, which is involved in chemoresistance.

View Article: PubMed Central - PubMed

Affiliation: Hepatobiliary and Pancreatic Surgery, Zhejiang Provincial People's Hospital, Hangzhou, China; Pancreatic Disease Institute, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Show MeSH
Related in: MedlinePlus