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ECT2 amplification and overexpression as a new prognostic biomarker for early-stage lung adenocarcinoma.

Murata Y, Minami Y, Iwakawa R, Yokota J, Usui S, Tsuta K, Shiraishi K, Sakashita S, Satomi K, Iijima T, Noguchi M - Cancer Sci. (2014)

Bottom Line: Array-comparative genomic hybridization indicated frequent amplification at chromosome 3q26.These results were verified using another set of early-stage adenocarcinomas resected at another hospital.Abnormality of the ECT2 gene occurs at a relatively early stage of lung adenocarcinogenesis and would be applicable as a new biomarker for prognostication of patients with lung adenocarcinoma.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology, Graduate School of Comprehensive Human Sciences, University of Tsukuba, Ibaraki, Japan.

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(a) Immunohistochemical (IHC) staining pattern of ECT2 and Ki-67. The same nuclei were stained by antibody against ECT2 and Ki-67. ECT2 in tumor cells was also stained weakly in the cytoplasm. Alveolar epithelial cells were used as a negative control. (b) FISH (left) and IHC images for one of the tumors showing high ECT2 amplification. Yellow arrows indicate the same nucleus. Red signal shows the ECT2 gene. Green signal shows chromosome 3 enumeration. IHC score, the count of stained nuclei in 1000 tumor cells; qPCR, quantitative real-time genomic PCR.
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fig02: (a) Immunohistochemical (IHC) staining pattern of ECT2 and Ki-67. The same nuclei were stained by antibody against ECT2 and Ki-67. ECT2 in tumor cells was also stained weakly in the cytoplasm. Alveolar epithelial cells were used as a negative control. (b) FISH (left) and IHC images for one of the tumors showing high ECT2 amplification. Yellow arrows indicate the same nucleus. Red signal shows the ECT2 gene. Green signal shows chromosome 3 enumeration. IHC score, the count of stained nuclei in 1000 tumor cells; qPCR, quantitative real-time genomic PCR.

Mentions: Using the 83 cases, IHC for ECT2, EIF5A2, PIK3CA, and TNFSF10 was carried out. Although faint staining was detected in the cytoplasm of the tumor cells, nuclei were mainly stained by antibody against ECT2 (Fig. 2a). For other genes, the cytoplasm or nuclear membrane was stained. The results showed a slight correlation with those of qPCR. The correlation coefficients were: r = 0.36 for ECT2; r = 0.07 for EIF5A2; r = 0.13 for PIK3CA; and r = 0.14 for TNFSF10. Of these four genes, ECT2 was correlated most closely with the qPCR value.


ECT2 amplification and overexpression as a new prognostic biomarker for early-stage lung adenocarcinoma.

Murata Y, Minami Y, Iwakawa R, Yokota J, Usui S, Tsuta K, Shiraishi K, Sakashita S, Satomi K, Iijima T, Noguchi M - Cancer Sci. (2014)

(a) Immunohistochemical (IHC) staining pattern of ECT2 and Ki-67. The same nuclei were stained by antibody against ECT2 and Ki-67. ECT2 in tumor cells was also stained weakly in the cytoplasm. Alveolar epithelial cells were used as a negative control. (b) FISH (left) and IHC images for one of the tumors showing high ECT2 amplification. Yellow arrows indicate the same nucleus. Red signal shows the ECT2 gene. Green signal shows chromosome 3 enumeration. IHC score, the count of stained nuclei in 1000 tumor cells; qPCR, quantitative real-time genomic PCR.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4317802&req=5

fig02: (a) Immunohistochemical (IHC) staining pattern of ECT2 and Ki-67. The same nuclei were stained by antibody against ECT2 and Ki-67. ECT2 in tumor cells was also stained weakly in the cytoplasm. Alveolar epithelial cells were used as a negative control. (b) FISH (left) and IHC images for one of the tumors showing high ECT2 amplification. Yellow arrows indicate the same nucleus. Red signal shows the ECT2 gene. Green signal shows chromosome 3 enumeration. IHC score, the count of stained nuclei in 1000 tumor cells; qPCR, quantitative real-time genomic PCR.
Mentions: Using the 83 cases, IHC for ECT2, EIF5A2, PIK3CA, and TNFSF10 was carried out. Although faint staining was detected in the cytoplasm of the tumor cells, nuclei were mainly stained by antibody against ECT2 (Fig. 2a). For other genes, the cytoplasm or nuclear membrane was stained. The results showed a slight correlation with those of qPCR. The correlation coefficients were: r = 0.36 for ECT2; r = 0.07 for EIF5A2; r = 0.13 for PIK3CA; and r = 0.14 for TNFSF10. Of these four genes, ECT2 was correlated most closely with the qPCR value.

Bottom Line: Array-comparative genomic hybridization indicated frequent amplification at chromosome 3q26.These results were verified using another set of early-stage adenocarcinomas resected at another hospital.Abnormality of the ECT2 gene occurs at a relatively early stage of lung adenocarcinogenesis and would be applicable as a new biomarker for prognostication of patients with lung adenocarcinoma.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology, Graduate School of Comprehensive Human Sciences, University of Tsukuba, Ibaraki, Japan.

Show MeSH
Related in: MedlinePlus