MKK7 mediates miR-493-dependent suppression of liver metastasis of colon cancer cells.
Bottom Line: MKK7 was expressed in six out of seven colon cancer cell lines examined but not in non-transformed colon epithelial cells, and its expression was required for the activating phosphorylation of JNK.RNA interference-mediated inhibition of MKK7 resulted in marked suppression of liver metastasis of colon cancer cells.Immunohistochemical examination in human primary colon tumors revealed that the occurrence of liver metastasis is associated with elevated levels of MKK7.
Affiliation: Division of Cancer Development System, National Cancer Center Research Institute, Tokyo, Japan.Show MeSH
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Mentions: In light of the functional association of MKK7 to metastasis (Figs 3, 4), we examined whether levels of MKK7 are associated with the presence of liver metastasis in clinical specimens. Human primary tumors with or without synchronous liver metastasis were immunostained with MKK7, and the extent of the staining was quantified (Fig. 5a). The comparison of the staining indicated that MKK7 was expressed in specimens with liver metastasis at levels higher than those without metastasis (Fig. 5b). These data are consistent with the role of MKK7 as a regulator of liver metastasis.
Affiliation: Division of Cancer Development System, National Cancer Center Research Institute, Tokyo, Japan.