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MKK7 mediates miR-493-dependent suppression of liver metastasis of colon cancer cells.

Sakai H, Sato A, Aihara Y, Ikarashi Y, Midorikawa Y, Kracht M, Nakagama H, Okamoto K - Cancer Sci. (2014)

Bottom Line: However, major functional targets that mediate the antimetastatic activity of miR-493 remain elusive.Here, we extended our search for target genes and identified MKK7, a mitogen-activated protein kinase kinase, as a novel target of miR-493. miR-493 inhibits MKK7 expression by targeting the binding site at the 3'-UTR of the mkk7 gene.Immunohistochemical examination in human primary colon tumors revealed that the occurrence of liver metastasis is associated with elevated levels of MKK7.

View Article: PubMed Central - PubMed

Affiliation: Division of Cancer Development System, National Cancer Center Research Institute, Tokyo, Japan.

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High levels of MKK7 in colon cancer are associated with liver metastasis. (a) representative immunostaining of MKK7 (grades 1 and 4) (b) Levels of MKK7 immunostaining of primary colon tumor without metastasis (meta) (−) or with synchronous metastasis (+).
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fig05: High levels of MKK7 in colon cancer are associated with liver metastasis. (a) representative immunostaining of MKK7 (grades 1 and 4) (b) Levels of MKK7 immunostaining of primary colon tumor without metastasis (meta) (−) or with synchronous metastasis (+).

Mentions: In light of the functional association of MKK7 to metastasis (Figs 3, 4), we examined whether levels of MKK7 are associated with the presence of liver metastasis in clinical specimens. Human primary tumors with or without synchronous liver metastasis were immunostained with MKK7, and the extent of the staining was quantified (Fig. 5a). The comparison of the staining indicated that MKK7 was expressed in specimens with liver metastasis at levels higher than those without metastasis (Fig. 5b). These data are consistent with the role of MKK7 as a regulator of liver metastasis.


MKK7 mediates miR-493-dependent suppression of liver metastasis of colon cancer cells.

Sakai H, Sato A, Aihara Y, Ikarashi Y, Midorikawa Y, Kracht M, Nakagama H, Okamoto K - Cancer Sci. (2014)

High levels of MKK7 in colon cancer are associated with liver metastasis. (a) representative immunostaining of MKK7 (grades 1 and 4) (b) Levels of MKK7 immunostaining of primary colon tumor without metastasis (meta) (−) or with synchronous metastasis (+).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4317799&req=5

fig05: High levels of MKK7 in colon cancer are associated with liver metastasis. (a) representative immunostaining of MKK7 (grades 1 and 4) (b) Levels of MKK7 immunostaining of primary colon tumor without metastasis (meta) (−) or with synchronous metastasis (+).
Mentions: In light of the functional association of MKK7 to metastasis (Figs 3, 4), we examined whether levels of MKK7 are associated with the presence of liver metastasis in clinical specimens. Human primary tumors with or without synchronous liver metastasis were immunostained with MKK7, and the extent of the staining was quantified (Fig. 5a). The comparison of the staining indicated that MKK7 was expressed in specimens with liver metastasis at levels higher than those without metastasis (Fig. 5b). These data are consistent with the role of MKK7 as a regulator of liver metastasis.

Bottom Line: However, major functional targets that mediate the antimetastatic activity of miR-493 remain elusive.Here, we extended our search for target genes and identified MKK7, a mitogen-activated protein kinase kinase, as a novel target of miR-493. miR-493 inhibits MKK7 expression by targeting the binding site at the 3'-UTR of the mkk7 gene.Immunohistochemical examination in human primary colon tumors revealed that the occurrence of liver metastasis is associated with elevated levels of MKK7.

View Article: PubMed Central - PubMed

Affiliation: Division of Cancer Development System, National Cancer Center Research Institute, Tokyo, Japan.

Show MeSH
Related in: MedlinePlus