MKK7 mediates miR-493-dependent suppression of liver metastasis of colon cancer cells.
Bottom Line: However, major functional targets that mediate the antimetastatic activity of miR-493 remain elusive.Here, we extended our search for target genes and identified MKK7, a mitogen-activated protein kinase kinase, as a novel target of miR-493. miR-493 inhibits MKK7 expression by targeting the binding site at the 3'-UTR of the mkk7 gene.Immunohistochemical examination in human primary colon tumors revealed that the occurrence of liver metastasis is associated with elevated levels of MKK7.
Affiliation: Division of Cancer Development System, National Cancer Center Research Institute, Tokyo, Japan.Show MeSH
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Mentions: Next, we examined MKK7 expression in colon cancer cell lines. Western blot analyses showed that MKK7 was expressed in six out of seven colon cancer cell lines examined (DLD-1, HCT116, HT29, RKO, SW480, and SW620), whereas the level of expression in non-transformed FHC colon epithelial cells was much lower than those in the cancer cells (Fig. 2a).
Affiliation: Division of Cancer Development System, National Cancer Center Research Institute, Tokyo, Japan.