MKK7 mediates miR-493-dependent suppression of liver metastasis of colon cancer cells.
Bottom Line: MKK7 was expressed in six out of seven colon cancer cell lines examined but not in non-transformed colon epithelial cells, and its expression was required for the activating phosphorylation of JNK.RNA interference-mediated inhibition of MKK7 resulted in marked suppression of liver metastasis of colon cancer cells.Immunohistochemical examination in human primary colon tumors revealed that the occurrence of liver metastasis is associated with elevated levels of MKK7.
Affiliation: Division of Cancer Development System, National Cancer Center Research Institute, Tokyo, Japan.Show MeSH
Related in: MedlinePlus
Mentions: Next, we examined MKK7 expression in colon cancer cell lines. Western blot analyses showed that MKK7 was expressed in six out of seven colon cancer cell lines examined (DLD-1, HCT116, HT29, RKO, SW480, and SW620), whereas the level of expression in non-transformed FHC colon epithelial cells was much lower than those in the cancer cells (Fig. 2a).
Affiliation: Division of Cancer Development System, National Cancer Center Research Institute, Tokyo, Japan.