Characterization of common marmoset dysgerminoma-like tumor induced by the lentiviral expression of reprogramming factors.
Bottom Line: Recent generation of induced pluripotent stem (iPSCs) has made a significant impact on the field of human regenerative medicine.Prior to the clinical application of iPSCs, testing of their safety and usefulness must be carried out using reliable animal models of various diseases.In order to generate iPSCs from common marmoset (CM; Callithrix jacchus), one of the most useful experimental animals, we have lentivirally transduced reprogramming factors, including POU5F1 (also known as OCT3/4), SOX2, KLF4, and c-MYC into CM fibroblasts.
Affiliation: Division of Molecular and Clinical Genetics, Molecular and Clinical Genetics, Medical Institute of Bioregulation, Kyushu University, Fukuoka, Japan.Show MeSH
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Mentions: Common marmoset ARCs, CM ESCs, and iPS A cells derived from fetal liver cells (provided by Erika Sasaki, KEIO-REKEN Research Center for Human Cognition, Keio University, Tokyo, Japan) were maintained in DMEM/F12 (Sigma-Aldrich, St. Louis, MO, USA) containing 20% Knockout Serum Replacement (Gibco, Carlsbad, CA, USA), 0.1 mM non-essential amino acid (Gibco), 1 mM L-glutamine (Nacalai Tesque, Kyoto, Japan), 1% antibiotic–antimycotics (Nacalai Tesque), 0.4 mM 2-mercaptoethanol (Sigma Aldrich), and 0.12% sodium hydroxide (Nacalai Tesque). The CM DGs were maintained in DMEM/F12 containing 10% FBS at 37°C in a 5% humidified CO2 atmosphere. Detailed descriptions of the cell culture, reprogramming method, and proliferation assay are provided in Figures 1 and 5.
Affiliation: Division of Molecular and Clinical Genetics, Molecular and Clinical Genetics, Medical Institute of Bioregulation, Kyushu University, Fukuoka, Japan.