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Characterization of common marmoset dysgerminoma-like tumor induced by the lentiviral expression of reprogramming factors.

Yamaguchi S, Marumoto T, Nii T, Kawano H, Liao J, Nagai Y, Okada M, Takahashi A, Inoue H, Sasaki E, Fujii H, Okano S, Ebise H, Sato T, Suyama M, Okano H, Miura Y, Tani K - Cancer Sci. (2014)

Bottom Line: Recent generation of induced pluripotent stem (iPSCs) has made a significant impact on the field of human regenerative medicine.Prior to the clinical application of iPSCs, testing of their safety and usefulness must be carried out using reliable animal models of various diseases.In order to generate iPSCs from common marmoset (CM; Callithrix jacchus), one of the most useful experimental animals, we have lentivirally transduced reprogramming factors, including POU5F1 (also known as OCT3/4), SOX2, KLF4, and c-MYC into CM fibroblasts.

View Article: PubMed Central - PubMed

Affiliation: Division of Molecular and Clinical Genetics, Molecular and Clinical Genetics, Medical Institute of Bioregulation, Kyushu University, Fukuoka, Japan.

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Effects of DNA-damaging agents and irradiation on common marmoset dysgerminoma-like cells (CM DGs). The cells were treated with (a) mitomycin C (MMC), (b) cisplatin, or (c) irradiation, and the proportions of sub-G1 populations in aorta-gonado-mesonephros (AGM) fibroblasts or CM DG cell lines were analyzed by FACS. Results are shown as means ± SD. *P < 0.05; **P < 0.01; ***P < 0.001.
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fig04: Effects of DNA-damaging agents and irradiation on common marmoset dysgerminoma-like cells (CM DGs). The cells were treated with (a) mitomycin C (MMC), (b) cisplatin, or (c) irradiation, and the proportions of sub-G1 populations in aorta-gonado-mesonephros (AGM) fibroblasts or CM DG cell lines were analyzed by FACS. Results are shown as means ± SD. *P < 0.05; **P < 0.01; ***P < 0.001.

Mentions: Dysgerminomas are generally sensitive to cisplatin and irradiation.(21,22) We therefore examined the effects of DNA-damaging agents such as MMC and cisplatin on CM DGs. Three CM DG cell lines (CMY402a, CMY402b, and CMY403a) were treated with MMC for 1 h, and the proportion of dead cells was analyzed by flow cytometry at 24 h after treatment. The percentage of cells with a sub-G1 DNA content was taken as a measure of dead cells in the population. The proportion of dead cells in MMC-treated CM DG cultures was significantly higher than that in controls (MMC-treated AGM fibroblasts) (Figs 4a, S7). Similar results were obtained when these three cell lines were treated with cisplatin or irradiation (Figs 4b,c, S8, S9). These results suggested that CM DGs were more sensitive to DNA damage than their parental AGM fibroblasts.


Characterization of common marmoset dysgerminoma-like tumor induced by the lentiviral expression of reprogramming factors.

Yamaguchi S, Marumoto T, Nii T, Kawano H, Liao J, Nagai Y, Okada M, Takahashi A, Inoue H, Sasaki E, Fujii H, Okano S, Ebise H, Sato T, Suyama M, Okano H, Miura Y, Tani K - Cancer Sci. (2014)

Effects of DNA-damaging agents and irradiation on common marmoset dysgerminoma-like cells (CM DGs). The cells were treated with (a) mitomycin C (MMC), (b) cisplatin, or (c) irradiation, and the proportions of sub-G1 populations in aorta-gonado-mesonephros (AGM) fibroblasts or CM DG cell lines were analyzed by FACS. Results are shown as means ± SD. *P < 0.05; **P < 0.01; ***P < 0.001.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4317795&req=5

fig04: Effects of DNA-damaging agents and irradiation on common marmoset dysgerminoma-like cells (CM DGs). The cells were treated with (a) mitomycin C (MMC), (b) cisplatin, or (c) irradiation, and the proportions of sub-G1 populations in aorta-gonado-mesonephros (AGM) fibroblasts or CM DG cell lines were analyzed by FACS. Results are shown as means ± SD. *P < 0.05; **P < 0.01; ***P < 0.001.
Mentions: Dysgerminomas are generally sensitive to cisplatin and irradiation.(21,22) We therefore examined the effects of DNA-damaging agents such as MMC and cisplatin on CM DGs. Three CM DG cell lines (CMY402a, CMY402b, and CMY403a) were treated with MMC for 1 h, and the proportion of dead cells was analyzed by flow cytometry at 24 h after treatment. The percentage of cells with a sub-G1 DNA content was taken as a measure of dead cells in the population. The proportion of dead cells in MMC-treated CM DG cultures was significantly higher than that in controls (MMC-treated AGM fibroblasts) (Figs 4a, S7). Similar results were obtained when these three cell lines were treated with cisplatin or irradiation (Figs 4b,c, S8, S9). These results suggested that CM DGs were more sensitive to DNA damage than their parental AGM fibroblasts.

Bottom Line: Recent generation of induced pluripotent stem (iPSCs) has made a significant impact on the field of human regenerative medicine.Prior to the clinical application of iPSCs, testing of their safety and usefulness must be carried out using reliable animal models of various diseases.In order to generate iPSCs from common marmoset (CM; Callithrix jacchus), one of the most useful experimental animals, we have lentivirally transduced reprogramming factors, including POU5F1 (also known as OCT3/4), SOX2, KLF4, and c-MYC into CM fibroblasts.

View Article: PubMed Central - PubMed

Affiliation: Division of Molecular and Clinical Genetics, Molecular and Clinical Genetics, Medical Institute of Bioregulation, Kyushu University, Fukuoka, Japan.

Show MeSH
Related in: MedlinePlus