Characterization of common marmoset dysgerminoma-like tumor induced by the lentiviral expression of reprogramming factors.
Bottom Line: Recent generation of induced pluripotent stem (iPSCs) has made a significant impact on the field of human regenerative medicine.Prior to the clinical application of iPSCs, testing of their safety and usefulness must be carried out using reliable animal models of various diseases.In order to generate iPSCs from common marmoset (CM; Callithrix jacchus), one of the most useful experimental animals, we have lentivirally transduced reprogramming factors, including POU5F1 (also known as OCT3/4), SOX2, KLF4, and c-MYC into CM fibroblasts.
Affiliation: Division of Molecular and Clinical Genetics, Molecular and Clinical Genetics, Medical Institute of Bioregulation, Kyushu University, Fukuoka, Japan.Show MeSH
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Mentions: Dysgerminomas are generally sensitive to cisplatin and irradiation.(21,22) We therefore examined the effects of DNA-damaging agents such as MMC and cisplatin on CM DGs. Three CM DG cell lines (CMY402a, CMY402b, and CMY403a) were treated with MMC for 1 h, and the proportion of dead cells was analyzed by flow cytometry at 24 h after treatment. The percentage of cells with a sub-G1 DNA content was taken as a measure of dead cells in the population. The proportion of dead cells in MMC-treated CM DG cultures was significantly higher than that in controls (MMC-treated AGM fibroblasts) (Figs 4a, S7). Similar results were obtained when these three cell lines were treated with cisplatin or irradiation (Figs 4b,c, S8, S9). These results suggested that CM DGs were more sensitive to DNA damage than their parental AGM fibroblasts.
Affiliation: Division of Molecular and Clinical Genetics, Molecular and Clinical Genetics, Medical Institute of Bioregulation, Kyushu University, Fukuoka, Japan.