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Characterization of common marmoset dysgerminoma-like tumor induced by the lentiviral expression of reprogramming factors.

Yamaguchi S, Marumoto T, Nii T, Kawano H, Liao J, Nagai Y, Okada M, Takahashi A, Inoue H, Sasaki E, Fujii H, Okano S, Ebise H, Sato T, Suyama M, Okano H, Miura Y, Tani K - Cancer Sci. (2014)

Bottom Line: Recent generation of induced pluripotent stem (iPSCs) has made a significant impact on the field of human regenerative medicine.Prior to the clinical application of iPSCs, testing of their safety and usefulness must be carried out using reliable animal models of various diseases.In order to generate iPSCs from common marmoset (CM; Callithrix jacchus), one of the most useful experimental animals, we have lentivirally transduced reprogramming factors, including POU5F1 (also known as OCT3/4), SOX2, KLF4, and c-MYC into CM fibroblasts.

View Article: PubMed Central - PubMed

Affiliation: Division of Molecular and Clinical Genetics, Molecular and Clinical Genetics, Medical Institute of Bioregulation, Kyushu University, Fukuoka, Japan.

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Characterization of common marmoset dysgerminoma-like (CM DG) cells in culture. (a) Representative phase-contrast image of CM DGs. (b) Immunofluorescent image of Venus expression in CM DGs. Bar = 100 μm. (c) Western blot analysis showing expression of reprogramming factors in CM DG cell lines. (d) RT-PCR analysis showing the expression of endogenous or exogenous reprogramming factors in CM DGs. Cj11 (CM embryonic stem cell line) was used as control.
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fig03: Characterization of common marmoset dysgerminoma-like (CM DG) cells in culture. (a) Representative phase-contrast image of CM DGs. (b) Immunofluorescent image of Venus expression in CM DGs. Bar = 100 μm. (c) Western blot analysis showing expression of reprogramming factors in CM DG cell lines. (d) RT-PCR analysis showing the expression of endogenous or exogenous reprogramming factors in CM DGs. Cj11 (CM embryonic stem cell line) was used as control.

Mentions: We then surgically removed CM DGs and cultured them in vitro to examine their biological characteristics. The CM DGs could grow infinitely in a semifloating state in the culture dish, and showed continuous expression of Venus fluorescent protein (Fig. 3a,b). We generated five CM DG cell lines (CMY401, CMY402a, CMY402b, CMY403a, and CMY403b) from five independent tumors formed by the injection of ARCs into SCID mice, and found that all four transduced reprogramming factors were integrated into their genomes (Fig. S6a). Both endogenous and exogenous reprogramming factors were expressed in these cell lines (Fig. 3c,d).


Characterization of common marmoset dysgerminoma-like tumor induced by the lentiviral expression of reprogramming factors.

Yamaguchi S, Marumoto T, Nii T, Kawano H, Liao J, Nagai Y, Okada M, Takahashi A, Inoue H, Sasaki E, Fujii H, Okano S, Ebise H, Sato T, Suyama M, Okano H, Miura Y, Tani K - Cancer Sci. (2014)

Characterization of common marmoset dysgerminoma-like (CM DG) cells in culture. (a) Representative phase-contrast image of CM DGs. (b) Immunofluorescent image of Venus expression in CM DGs. Bar = 100 μm. (c) Western blot analysis showing expression of reprogramming factors in CM DG cell lines. (d) RT-PCR analysis showing the expression of endogenous or exogenous reprogramming factors in CM DGs. Cj11 (CM embryonic stem cell line) was used as control.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4317795&req=5

fig03: Characterization of common marmoset dysgerminoma-like (CM DG) cells in culture. (a) Representative phase-contrast image of CM DGs. (b) Immunofluorescent image of Venus expression in CM DGs. Bar = 100 μm. (c) Western blot analysis showing expression of reprogramming factors in CM DG cell lines. (d) RT-PCR analysis showing the expression of endogenous or exogenous reprogramming factors in CM DGs. Cj11 (CM embryonic stem cell line) was used as control.
Mentions: We then surgically removed CM DGs and cultured them in vitro to examine their biological characteristics. The CM DGs could grow infinitely in a semifloating state in the culture dish, and showed continuous expression of Venus fluorescent protein (Fig. 3a,b). We generated five CM DG cell lines (CMY401, CMY402a, CMY402b, CMY403a, and CMY403b) from five independent tumors formed by the injection of ARCs into SCID mice, and found that all four transduced reprogramming factors were integrated into their genomes (Fig. S6a). Both endogenous and exogenous reprogramming factors were expressed in these cell lines (Fig. 3c,d).

Bottom Line: Recent generation of induced pluripotent stem (iPSCs) has made a significant impact on the field of human regenerative medicine.Prior to the clinical application of iPSCs, testing of their safety and usefulness must be carried out using reliable animal models of various diseases.In order to generate iPSCs from common marmoset (CM; Callithrix jacchus), one of the most useful experimental animals, we have lentivirally transduced reprogramming factors, including POU5F1 (also known as OCT3/4), SOX2, KLF4, and c-MYC into CM fibroblasts.

View Article: PubMed Central - PubMed

Affiliation: Division of Molecular and Clinical Genetics, Molecular and Clinical Genetics, Medical Institute of Bioregulation, Kyushu University, Fukuoka, Japan.

Show MeSH
Related in: MedlinePlus