Characterization of common marmoset dysgerminoma-like tumor induced by the lentiviral expression of reprogramming factors.
Bottom Line: Recent generation of induced pluripotent stem (iPSCs) has made a significant impact on the field of human regenerative medicine.Prior to the clinical application of iPSCs, testing of their safety and usefulness must be carried out using reliable animal models of various diseases.In order to generate iPSCs from common marmoset (CM; Callithrix jacchus), one of the most useful experimental animals, we have lentivirally transduced reprogramming factors, including POU5F1 (also known as OCT3/4), SOX2, KLF4, and c-MYC into CM fibroblasts.
Affiliation: Division of Molecular and Clinical Genetics, Molecular and Clinical Genetics, Medical Institute of Bioregulation, Kyushu University, Fukuoka, Japan.Show MeSH
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Mentions: Total RNA from AGM fibroblasts, ARCs, and iPS A cells were isolated using the RNeasy Mini Kit (Qiagen, Valencia, CA, USA). RNA was reverse-transcribed, biotin-labeled, and hybridized for 16 h to a marmoset genome oligonucleotide custom array Marmo2 (in preparation)(12), which was subsequently washed and stained in a Fluidics Station 450 (Affymetrix, Santa Clara, CA, USA) according to the manufacturer's instructions. Detailed protocols of microarray analysis are provided in Figures 2 and S5.
Affiliation: Division of Molecular and Clinical Genetics, Molecular and Clinical Genetics, Medical Institute of Bioregulation, Kyushu University, Fukuoka, Japan.