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Methylation-induced downregulation of TFPI-2 causes TMPRSS4 overexpression and contributes to oncogenesis in a subset of non-small-cell lung carcinoma.

Hamamoto J, Soejima K, Naoki K, Yasuda H, Hayashi Y, Yoda S, Nakayama S, Satomi R, Terai H, Ikemura S, Sato T, Arai D, Ishioka K, Ohgino K, Betsuyaku T - Cancer Sci. (2014)

Bottom Line: Interestingly, we found that TMPRSS4 expression was associated with tissue factor pathway inhibitor 2 (TFPI-2) expression in these clinical samples.Knockdown of TMPRSS4 reduced the proliferation rate in several lung cancer cell lines.We found a novel molecular mechanism that TFPI-2 negatively regulates cell growth by inhibiting transcription of TMPRSS4.

View Article: PubMed Central - PubMed

Affiliation: Department of Pulmonary Medicine, School of Medicine, Keio University, Tokyo, Japan.

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Effect of knockdown or overexpression of TMPRSS4 on lung cancer cell growth. NCI-H358, NCI-H520, NCI-H1975, A549, and NCI-H2228 cells were seeded at 5000 cells/well for NCI-H358 and 1000 cells/well for other cell lines. TMPRSS4 siRNA (a) or a TMPRSS4 overexpression plasmid (b) was transfected the next day. Cell growth was measured using a proliferation assay from day 0 to day 6 and calculated as fold change from day 0. *P < 0.05 compared to negative siRNA or Flag plasmid alone. All experiments were carried out in triplicate. Data are representative of three independent experiments.
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fig04: Effect of knockdown or overexpression of TMPRSS4 on lung cancer cell growth. NCI-H358, NCI-H520, NCI-H1975, A549, and NCI-H2228 cells were seeded at 5000 cells/well for NCI-H358 and 1000 cells/well for other cell lines. TMPRSS4 siRNA (a) or a TMPRSS4 overexpression plasmid (b) was transfected the next day. Cell growth was measured using a proliferation assay from day 0 to day 6 and calculated as fold change from day 0. *P < 0.05 compared to negative siRNA or Flag plasmid alone. All experiments were carried out in triplicate. Data are representative of three independent experiments.

Mentions: Next, a proliferation assay was carried out to assess the effect of TMPRSS4 on cell growth by knockdown or overexpression of the gene. The knockdown of TMPRSS4 expression by siRNA treatment inhibited growth in all of five tested cell lines, NCI-H358, NCI-H520, NCI-H1975, A549, and NCI-H2228 (Fig.4a); conversely, the overexpression of TMPRSS4 augmented the growth of these cell lines (Fig.4b).


Methylation-induced downregulation of TFPI-2 causes TMPRSS4 overexpression and contributes to oncogenesis in a subset of non-small-cell lung carcinoma.

Hamamoto J, Soejima K, Naoki K, Yasuda H, Hayashi Y, Yoda S, Nakayama S, Satomi R, Terai H, Ikemura S, Sato T, Arai D, Ishioka K, Ohgino K, Betsuyaku T - Cancer Sci. (2014)

Effect of knockdown or overexpression of TMPRSS4 on lung cancer cell growth. NCI-H358, NCI-H520, NCI-H1975, A549, and NCI-H2228 cells were seeded at 5000 cells/well for NCI-H358 and 1000 cells/well for other cell lines. TMPRSS4 siRNA (a) or a TMPRSS4 overexpression plasmid (b) was transfected the next day. Cell growth was measured using a proliferation assay from day 0 to day 6 and calculated as fold change from day 0. *P < 0.05 compared to negative siRNA or Flag plasmid alone. All experiments were carried out in triplicate. Data are representative of three independent experiments.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4317784&req=5

fig04: Effect of knockdown or overexpression of TMPRSS4 on lung cancer cell growth. NCI-H358, NCI-H520, NCI-H1975, A549, and NCI-H2228 cells were seeded at 5000 cells/well for NCI-H358 and 1000 cells/well for other cell lines. TMPRSS4 siRNA (a) or a TMPRSS4 overexpression plasmid (b) was transfected the next day. Cell growth was measured using a proliferation assay from day 0 to day 6 and calculated as fold change from day 0. *P < 0.05 compared to negative siRNA or Flag plasmid alone. All experiments were carried out in triplicate. Data are representative of three independent experiments.
Mentions: Next, a proliferation assay was carried out to assess the effect of TMPRSS4 on cell growth by knockdown or overexpression of the gene. The knockdown of TMPRSS4 expression by siRNA treatment inhibited growth in all of five tested cell lines, NCI-H358, NCI-H520, NCI-H1975, A549, and NCI-H2228 (Fig.4a); conversely, the overexpression of TMPRSS4 augmented the growth of these cell lines (Fig.4b).

Bottom Line: Interestingly, we found that TMPRSS4 expression was associated with tissue factor pathway inhibitor 2 (TFPI-2) expression in these clinical samples.Knockdown of TMPRSS4 reduced the proliferation rate in several lung cancer cell lines.We found a novel molecular mechanism that TFPI-2 negatively regulates cell growth by inhibiting transcription of TMPRSS4.

View Article: PubMed Central - PubMed

Affiliation: Department of Pulmonary Medicine, School of Medicine, Keio University, Tokyo, Japan.

Show MeSH
Related in: MedlinePlus