Methylation-induced downregulation of TFPI-2 causes TMPRSS4 overexpression and contributes to oncogenesis in a subset of non-small-cell lung carcinoma.
Bottom Line: Interestingly, we found that TMPRSS4 expression was associated with tissue factor pathway inhibitor 2 (TFPI-2) expression in these clinical samples.Knockdown of TMPRSS4 reduced the proliferation rate in several lung cancer cell lines.We found a novel molecular mechanism that TFPI-2 negatively regulates cell growth by inhibiting transcription of TMPRSS4.
Affiliation: Department of Pulmonary Medicine, School of Medicine, Keio University, Tokyo, Japan.Show MeSH
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Mentions: Next, a proliferation assay was carried out to assess the effect of TMPRSS4 on cell growth by knockdown or overexpression of the gene. The knockdown of TMPRSS4 expression by siRNA treatment inhibited growth in all of five tested cell lines, NCI-H358, NCI-H520, NCI-H1975, A549, and NCI-H2228 (Fig.4a); conversely, the overexpression of TMPRSS4 augmented the growth of these cell lines (Fig.4b).
Affiliation: Department of Pulmonary Medicine, School of Medicine, Keio University, Tokyo, Japan.