High-dose chemotherapeutics of intravesical chemotherapy rapidly induce mitochondrial dysfunction in bladder cancer-derived spheroids.
Bottom Line: Two hours after exposure to MMC, the mitochondrial membrane potential decreased and the mitochondria were fragmented, while plasma membrane integrity was maintained.ATP levels rapidly decreased in CTOS after exposure to EPI or MMC.In the CTOS prepared directly from 19 surgical specimens exposed to EPI and MMC, the decrease of ATP levels varied among patients.
Affiliation: Department of Biochemistry, Osaka Medical Center for Cancer and Cardiovascular Diseases, Osaka, Japan; Department of Urology, Osaka University Graduate School of Medicine, Osaka, Japan.Show MeSH
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Mentions: We previously reported the preparation and culture of bladder cancer cells from surgically resected samples based on the CTOS method.24 Here, we focused on CTOS from TUR samples. CTOS were prepared from 176 TUR samples, including 135 from the previous study. More than 20 CTOSs were obtained from each sample; the success rate of CTOS preparation was better in NMIBC (76.2%) than in MIBC (42.1%) (Fig.1a). Low-grade (in pathological findings) and papillary (in cystoscopic appearance) types had better success rates than others. The CTOS from high-grade tumors were irregularly shaped, with a high nucleus-to-cytoplasm ratio (Fig.1b). In contrast, CTOS from low-grade tumors were round, with a low nucleus-to-cytoplasm ratio. These findings were comparable to the original tumors. There was no correlation between clinicopathological factors and CTOS growth in 14 patient samples examined (Suppl. Table S1).
Affiliation: Department of Biochemistry, Osaka Medical Center for Cancer and Cardiovascular Diseases, Osaka, Japan; Department of Urology, Osaka University Graduate School of Medicine, Osaka, Japan.