Microparticles induce multifactorial resistance through oncogenic pathways independently of cancer cell type.
Bottom Line: By co-culturing MP derived from MDR-positive cells with recipient cells, we showed that sensitive cells accumulated Pgp, IAP proteins and mRNA.In addition, MP promoted microRNA transfer and NFκB and Yb-1 activation.Therefore, our results indicate that MP can induce a multifactorial phenotype in sensitive cancer cells.
Affiliation: Program of Hemato-Oncology Molecular, Brazilian National Cancer Institute, Rio de Janeiro, Brazil.Show MeSH
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Mentions: In MDR cell-derived MP, we analyzed soluble proteins (cIAP-1, XIAP and survivin) and RNA (mRNA and miRNA) that are involved in the multifactorial resistant phenotype. First, our data revealed that CML MDR-positive cell-derived MP contained anti-apoptotic proteins, such as survivin, cIAP-1 and a small amount of XIAP (Fig.2a). Their respective mRNA, BIRC5 and BIRC2, but not BIRC4, were contained in MP in addition to ABCB1 mRNA (Fig.2b).
Affiliation: Program of Hemato-Oncology Molecular, Brazilian National Cancer Institute, Rio de Janeiro, Brazil.