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Microparticles induce multifactorial resistance through oncogenic pathways independently of cancer cell type.

de Souza PS, Cruz AL, Viola JP, Maia RC - Cancer Sci. (2014)

Bottom Line: By co-culturing MP derived from MDR-positive cells with recipient cells, we showed that sensitive cells accumulated Pgp, IAP proteins and mRNA.In addition, MP promoted microRNA transfer and NFκB and Yb-1 activation.Therefore, our results indicate that MP can induce a multifactorial phenotype in sensitive cancer cells.

View Article: PubMed Central - PubMed

Affiliation: Program of Hemato-Oncology Molecular, Brazilian National Cancer Institute, Rio de Janeiro, Brazil.

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Related in: MedlinePlus

Microparticles derived from multidrug resistance (MDR)-cells transport resistance proteins and RNA molecules. cIAP-1, survivin and XIAP protein levels of Lucena cells and MDR-positive membrane microparticles (MP) were analyzed by western blot. β-actin was used as load control (a). ABCB1,BIRC2,BIRC5 and BIRC4mRNA levels were analyzed in Lucena cells and MDR-positive MP by TaqMan quantitative RT-PCR. The mRNA were normalized to β-actin mRNA expression (b). miR-21, miR-451 and miR-27a levels were determined in Lucena cells and MDR-positive MP. These miRNA were normalized to RNUB6 (c). Each bar represents the mean ± SD of triplicate experiments.
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fig02: Microparticles derived from multidrug resistance (MDR)-cells transport resistance proteins and RNA molecules. cIAP-1, survivin and XIAP protein levels of Lucena cells and MDR-positive membrane microparticles (MP) were analyzed by western blot. β-actin was used as load control (a). ABCB1,BIRC2,BIRC5 and BIRC4mRNA levels were analyzed in Lucena cells and MDR-positive MP by TaqMan quantitative RT-PCR. The mRNA were normalized to β-actin mRNA expression (b). miR-21, miR-451 and miR-27a levels were determined in Lucena cells and MDR-positive MP. These miRNA were normalized to RNUB6 (c). Each bar represents the mean ± SD of triplicate experiments.

Mentions: In MDR cell-derived MP, we analyzed soluble proteins (cIAP-1, XIAP and survivin) and RNA (mRNA and miRNA) that are involved in the multifactorial resistant phenotype. First, our data revealed that CML MDR-positive cell-derived MP contained anti-apoptotic proteins, such as survivin, cIAP-1 and a small amount of XIAP (Fig.2a). Their respective mRNA, BIRC5 and BIRC2, but not BIRC4, were contained in MP in addition to ABCB1 mRNA (Fig.2b).


Microparticles induce multifactorial resistance through oncogenic pathways independently of cancer cell type.

de Souza PS, Cruz AL, Viola JP, Maia RC - Cancer Sci. (2014)

Microparticles derived from multidrug resistance (MDR)-cells transport resistance proteins and RNA molecules. cIAP-1, survivin and XIAP protein levels of Lucena cells and MDR-positive membrane microparticles (MP) were analyzed by western blot. β-actin was used as load control (a). ABCB1,BIRC2,BIRC5 and BIRC4mRNA levels were analyzed in Lucena cells and MDR-positive MP by TaqMan quantitative RT-PCR. The mRNA were normalized to β-actin mRNA expression (b). miR-21, miR-451 and miR-27a levels were determined in Lucena cells and MDR-positive MP. These miRNA were normalized to RNUB6 (c). Each bar represents the mean ± SD of triplicate experiments.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4317771&req=5

fig02: Microparticles derived from multidrug resistance (MDR)-cells transport resistance proteins and RNA molecules. cIAP-1, survivin and XIAP protein levels of Lucena cells and MDR-positive membrane microparticles (MP) were analyzed by western blot. β-actin was used as load control (a). ABCB1,BIRC2,BIRC5 and BIRC4mRNA levels were analyzed in Lucena cells and MDR-positive MP by TaqMan quantitative RT-PCR. The mRNA were normalized to β-actin mRNA expression (b). miR-21, miR-451 and miR-27a levels were determined in Lucena cells and MDR-positive MP. These miRNA were normalized to RNUB6 (c). Each bar represents the mean ± SD of triplicate experiments.
Mentions: In MDR cell-derived MP, we analyzed soluble proteins (cIAP-1, XIAP and survivin) and RNA (mRNA and miRNA) that are involved in the multifactorial resistant phenotype. First, our data revealed that CML MDR-positive cell-derived MP contained anti-apoptotic proteins, such as survivin, cIAP-1 and a small amount of XIAP (Fig.2a). Their respective mRNA, BIRC5 and BIRC2, but not BIRC4, were contained in MP in addition to ABCB1 mRNA (Fig.2b).

Bottom Line: By co-culturing MP derived from MDR-positive cells with recipient cells, we showed that sensitive cells accumulated Pgp, IAP proteins and mRNA.In addition, MP promoted microRNA transfer and NFκB and Yb-1 activation.Therefore, our results indicate that MP can induce a multifactorial phenotype in sensitive cancer cells.

View Article: PubMed Central - PubMed

Affiliation: Program of Hemato-Oncology Molecular, Brazilian National Cancer Institute, Rio de Janeiro, Brazil.

Show MeSH
Related in: MedlinePlus