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Inducing malignant transformation of endometriosis in rats by long-term sustaining hyperestrogenemia and type II diabetes.

Wang CT, Wang DB, Liu KR, Li Y, Sun CX, Guo CS, Ren F - Cancer Sci. (2014)

Bottom Line: The same tendency was found in the corresponding eutopic endometria.The induced malignant endometriosis showed similarities with human disease in the pathological process and histomorphological and molecular biological features.The method is feasible.

View Article: PubMed Central - PubMed

Affiliation: Department of Obstetrics and Gynecology, Shengjing Hospital, China Medical University, Shenyang, China.

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H-score value of apoptosis and proliferation of endometriosis (EMs) lesions in a rat model of malignant transformation, expressed as mean ± SEM. *Compared with EMs and atypical EMs of the Es group (treated with estradiol [5 mg/kg three times/week after surgery], streptozotocin [25 mg/kg, 1 month after surgery], and high carbohydrate-and-fat feed), P < 0.01. #Compared to EMs of the control group (placebo saline and standard feed), P < 0.05. (a, e) EMs in the control group (400×); (b, f) EMs in the Es group (400×); (c, g) atypical EMs in the Es group (400×); (d, h) malignant EMs in the Es group (400×).
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fig02: H-score value of apoptosis and proliferation of endometriosis (EMs) lesions in a rat model of malignant transformation, expressed as mean ± SEM. *Compared with EMs and atypical EMs of the Es group (treated with estradiol [5 mg/kg three times/week after surgery], streptozotocin [25 mg/kg, 1 month after surgery], and high carbohydrate-and-fat feed), P < 0.01. #Compared to EMs of the control group (placebo saline and standard feed), P < 0.05. (a, e) EMs in the control group (400×); (b, f) EMs in the Es group (400×); (c, g) atypical EMs in the Es group (400×); (d, h) malignant EMs in the Es group (400×).

Mentions: The atrophy and simple hyperplastic EMs were excluded from the proliferation and apoptosis analysis. In the Es group, the immunostaining of PCNA protein increased (P < 0.01), and the level of TUNEL positivity decreased (P < 0.01) progressively in the order of EMs, atypical EMs, and malignant EMs. The immunostaining of PCNA in EMs of the Es group was stronger than that of the control group (P < 0.05), and the staining intensity of apoptosis in the Es group was lower than that of the control group (P < 0.05) (Fig.2).


Inducing malignant transformation of endometriosis in rats by long-term sustaining hyperestrogenemia and type II diabetes.

Wang CT, Wang DB, Liu KR, Li Y, Sun CX, Guo CS, Ren F - Cancer Sci. (2014)

H-score value of apoptosis and proliferation of endometriosis (EMs) lesions in a rat model of malignant transformation, expressed as mean ± SEM. *Compared with EMs and atypical EMs of the Es group (treated with estradiol [5 mg/kg three times/week after surgery], streptozotocin [25 mg/kg, 1 month after surgery], and high carbohydrate-and-fat feed), P < 0.01. #Compared to EMs of the control group (placebo saline and standard feed), P < 0.05. (a, e) EMs in the control group (400×); (b, f) EMs in the Es group (400×); (c, g) atypical EMs in the Es group (400×); (d, h) malignant EMs in the Es group (400×).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4317770&req=5

fig02: H-score value of apoptosis and proliferation of endometriosis (EMs) lesions in a rat model of malignant transformation, expressed as mean ± SEM. *Compared with EMs and atypical EMs of the Es group (treated with estradiol [5 mg/kg three times/week after surgery], streptozotocin [25 mg/kg, 1 month after surgery], and high carbohydrate-and-fat feed), P < 0.01. #Compared to EMs of the control group (placebo saline and standard feed), P < 0.05. (a, e) EMs in the control group (400×); (b, f) EMs in the Es group (400×); (c, g) atypical EMs in the Es group (400×); (d, h) malignant EMs in the Es group (400×).
Mentions: The atrophy and simple hyperplastic EMs were excluded from the proliferation and apoptosis analysis. In the Es group, the immunostaining of PCNA protein increased (P < 0.01), and the level of TUNEL positivity decreased (P < 0.01) progressively in the order of EMs, atypical EMs, and malignant EMs. The immunostaining of PCNA in EMs of the Es group was stronger than that of the control group (P < 0.05), and the staining intensity of apoptosis in the Es group was lower than that of the control group (P < 0.05) (Fig.2).

Bottom Line: The same tendency was found in the corresponding eutopic endometria.The induced malignant endometriosis showed similarities with human disease in the pathological process and histomorphological and molecular biological features.The method is feasible.

View Article: PubMed Central - PubMed

Affiliation: Department of Obstetrics and Gynecology, Shengjing Hospital, China Medical University, Shenyang, China.

Show MeSH
Related in: MedlinePlus