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Inducing malignant transformation of endometriosis in rats by long-term sustaining hyperestrogenemia and type II diabetes.

Wang CT, Wang DB, Liu KR, Li Y, Sun CX, Guo CS, Ren F - Cancer Sci. (2014)

Bottom Line: The same tendency was found in the corresponding eutopic endometria.The induced malignant endometriosis showed similarities with human disease in the pathological process and histomorphological and molecular biological features.The method is feasible.

View Article: PubMed Central - PubMed

Affiliation: Department of Obstetrics and Gynecology, Shengjing Hospital, China Medical University, Shenyang, China.

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Related in: MedlinePlus

Rat model of malignant transformation in surgically induced endometriosis (EMs). (a) Distinctively enlarged EMs lesion. (b) Viable EMs lesions of the control group (placebo saline and standard feed). (c) EMs of the control group (100×; 400×). (d) EMs with simple hyperplasia showing that the epithelial cells were increased in number, enlarged in size, and distributed in multiple layers, without atypical nuclei (100×; 400×). (e) Atypical EMs showing that the number of endometrial glands was increased, multiple layers were arranged compactly, cytoplasm was reduced, and the cell nuclei were enlarged and moderately irregular (100×; 400×). (f) Malignant transformation of EMs (endometrioid adenocarcinoma): the number of endometrial glands was increased extraordinarily with a disordered arrangement of cells, infiltration of inflammatory cells, reduced cytoplasm, and condensed nuclei irregular in shape and size with the back-to-back phenomenon. There were also bleeding points (100×; 400×). (g) Atrophy EMs showed an apparent decrease of epithelial and stromal cells (100×; 400×). (h, i) Ultrastructure of EMs. (h) Malignant endometriotic cells showing vacuole changes of mitochondria (arrow, 5000×). (i) Simple hyperplastic endometriotic cells of the Es group, treated with estradiol (5 mg/kg three times/week after surgery), streptozotocin (25 mg/kg, 1 month after surgery), and high carbohydrate-and-fat feed, at month 8 showing clearly enlarged and well-defined nuclei with reduced cytoplasm (arrow, 6000×).
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fig01: Rat model of malignant transformation in surgically induced endometriosis (EMs). (a) Distinctively enlarged EMs lesion. (b) Viable EMs lesions of the control group (placebo saline and standard feed). (c) EMs of the control group (100×; 400×). (d) EMs with simple hyperplasia showing that the epithelial cells were increased in number, enlarged in size, and distributed in multiple layers, without atypical nuclei (100×; 400×). (e) Atypical EMs showing that the number of endometrial glands was increased, multiple layers were arranged compactly, cytoplasm was reduced, and the cell nuclei were enlarged and moderately irregular (100×; 400×). (f) Malignant transformation of EMs (endometrioid adenocarcinoma): the number of endometrial glands was increased extraordinarily with a disordered arrangement of cells, infiltration of inflammatory cells, reduced cytoplasm, and condensed nuclei irregular in shape and size with the back-to-back phenomenon. There were also bleeding points (100×; 400×). (g) Atrophy EMs showed an apparent decrease of epithelial and stromal cells (100×; 400×). (h, i) Ultrastructure of EMs. (h) Malignant endometriotic cells showing vacuole changes of mitochondria (arrow, 5000×). (i) Simple hyperplastic endometriotic cells of the Es group, treated with estradiol (5 mg/kg three times/week after surgery), streptozotocin (25 mg/kg, 1 month after surgery), and high carbohydrate-and-fat feed, at month 8 showing clearly enlarged and well-defined nuclei with reduced cytoplasm (arrow, 6000×).

Mentions: Two cases of EMs in the Es group (8 months after surgery) were extraordinarily enlarged (Fig.1a). Grossly, the two were cystic, consisted of thickened capsule wall with papillary hyperplasia and sanguinopurulent fluid (H&E stain revealed malignancy). Endometriosis of the control group (Fig.1b) had the appearance of unilocular cystic structures filled with clear fluid. The uteri of the Es group showed severe edema, with no papillary hyperplasia, whereas uteri of the control group showed no obvious abnormality.


Inducing malignant transformation of endometriosis in rats by long-term sustaining hyperestrogenemia and type II diabetes.

Wang CT, Wang DB, Liu KR, Li Y, Sun CX, Guo CS, Ren F - Cancer Sci. (2014)

Rat model of malignant transformation in surgically induced endometriosis (EMs). (a) Distinctively enlarged EMs lesion. (b) Viable EMs lesions of the control group (placebo saline and standard feed). (c) EMs of the control group (100×; 400×). (d) EMs with simple hyperplasia showing that the epithelial cells were increased in number, enlarged in size, and distributed in multiple layers, without atypical nuclei (100×; 400×). (e) Atypical EMs showing that the number of endometrial glands was increased, multiple layers were arranged compactly, cytoplasm was reduced, and the cell nuclei were enlarged and moderately irregular (100×; 400×). (f) Malignant transformation of EMs (endometrioid adenocarcinoma): the number of endometrial glands was increased extraordinarily with a disordered arrangement of cells, infiltration of inflammatory cells, reduced cytoplasm, and condensed nuclei irregular in shape and size with the back-to-back phenomenon. There were also bleeding points (100×; 400×). (g) Atrophy EMs showed an apparent decrease of epithelial and stromal cells (100×; 400×). (h, i) Ultrastructure of EMs. (h) Malignant endometriotic cells showing vacuole changes of mitochondria (arrow, 5000×). (i) Simple hyperplastic endometriotic cells of the Es group, treated with estradiol (5 mg/kg three times/week after surgery), streptozotocin (25 mg/kg, 1 month after surgery), and high carbohydrate-and-fat feed, at month 8 showing clearly enlarged and well-defined nuclei with reduced cytoplasm (arrow, 6000×).
© Copyright Policy - open-access
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4317770&req=5

fig01: Rat model of malignant transformation in surgically induced endometriosis (EMs). (a) Distinctively enlarged EMs lesion. (b) Viable EMs lesions of the control group (placebo saline and standard feed). (c) EMs of the control group (100×; 400×). (d) EMs with simple hyperplasia showing that the epithelial cells were increased in number, enlarged in size, and distributed in multiple layers, without atypical nuclei (100×; 400×). (e) Atypical EMs showing that the number of endometrial glands was increased, multiple layers were arranged compactly, cytoplasm was reduced, and the cell nuclei were enlarged and moderately irregular (100×; 400×). (f) Malignant transformation of EMs (endometrioid adenocarcinoma): the number of endometrial glands was increased extraordinarily with a disordered arrangement of cells, infiltration of inflammatory cells, reduced cytoplasm, and condensed nuclei irregular in shape and size with the back-to-back phenomenon. There were also bleeding points (100×; 400×). (g) Atrophy EMs showed an apparent decrease of epithelial and stromal cells (100×; 400×). (h, i) Ultrastructure of EMs. (h) Malignant endometriotic cells showing vacuole changes of mitochondria (arrow, 5000×). (i) Simple hyperplastic endometriotic cells of the Es group, treated with estradiol (5 mg/kg three times/week after surgery), streptozotocin (25 mg/kg, 1 month after surgery), and high carbohydrate-and-fat feed, at month 8 showing clearly enlarged and well-defined nuclei with reduced cytoplasm (arrow, 6000×).
Mentions: Two cases of EMs in the Es group (8 months after surgery) were extraordinarily enlarged (Fig.1a). Grossly, the two were cystic, consisted of thickened capsule wall with papillary hyperplasia and sanguinopurulent fluid (H&E stain revealed malignancy). Endometriosis of the control group (Fig.1b) had the appearance of unilocular cystic structures filled with clear fluid. The uteri of the Es group showed severe edema, with no papillary hyperplasia, whereas uteri of the control group showed no obvious abnormality.

Bottom Line: The same tendency was found in the corresponding eutopic endometria.The induced malignant endometriosis showed similarities with human disease in the pathological process and histomorphological and molecular biological features.The method is feasible.

View Article: PubMed Central - PubMed

Affiliation: Department of Obstetrics and Gynecology, Shengjing Hospital, China Medical University, Shenyang, China.

Show MeSH
Related in: MedlinePlus