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Disruption of medial prefrontal synchrony in the subchronic phencyclidine model of schizophrenia in rats.

Young AM, Stubbendorff C, Valencia M, Gerdjikov TV - Neuroscience (2014)

Bottom Line: We found that this regimen reduced theta oscillations in the medial prefrontal cortex.It further produced abnormal cortical synchronization in putative cortical pyramidal cells.These alterations in prefrontal cortex functioning may contribute to cognitive deficits seen in subchronic NMDA antagonist pre-treated animals in prefrontal-dependent tasks.

View Article: PubMed Central - PubMed

Affiliation: School of Psychology, College of Medicine, Biological Sciences and Psychology, University of Leicester, United Kingdom.

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mPFC putative pyramidal neurons show enhanced synchrony with lower LFP frequencies in PCP-treated animals. (A) Example of single-unit activity in a neuron recorded from a PCP-treated animal (top) juxtaposed to ongoing LFP oscillations (middle) recorded from the same electrode and filtered at 1–5 Hz (green trace). The instantaneous phase (bottom) of the 1–5-Hz oscillations is obtained using a Hilbert transformation. It was used to calculate resultant vector length for spike times relative to the phases of the 1–5-Hz frequency band as shown in the polar plot on the right. Resultant vector length reflects the magnitude of the directionality effect and thus represents the degree of locking. (B) Example of single-unit activity juxtaposed to ongoing LFP oscillations in a neuron recorded from a saline-treated animal; data are represented as in (A). (C) Population locking of single units across frequencies for PCP and saline-treated animals. Neurons recorded in PCP-treated animals showed enhanced locking to lower frequencies. Error bands are calculated across neurons and represent SEM. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
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f0015: mPFC putative pyramidal neurons show enhanced synchrony with lower LFP frequencies in PCP-treated animals. (A) Example of single-unit activity in a neuron recorded from a PCP-treated animal (top) juxtaposed to ongoing LFP oscillations (middle) recorded from the same electrode and filtered at 1–5 Hz (green trace). The instantaneous phase (bottom) of the 1–5-Hz oscillations is obtained using a Hilbert transformation. It was used to calculate resultant vector length for spike times relative to the phases of the 1–5-Hz frequency band as shown in the polar plot on the right. Resultant vector length reflects the magnitude of the directionality effect and thus represents the degree of locking. (B) Example of single-unit activity juxtaposed to ongoing LFP oscillations in a neuron recorded from a saline-treated animal; data are represented as in (A). (C) Population locking of single units across frequencies for PCP and saline-treated animals. Neurons recorded in PCP-treated animals showed enhanced locking to lower frequencies. Error bands are calculated across neurons and represent SEM. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

Mentions: mPFC was targeted with a left-side craniotomy with coordinates: +3.2 mm AP; 0.5 mm ML; −2 mm DV (Paxinos and Watson, 2007). Recording electrodes consisted of quartz glass-coated platinum/tungsten wires pulled and ground to custom shapes in our laboratory (shank diameter 80 μm; diameter of the metal core 23 μm; free tip length ∼8 μm; impedance, 1–3 MΩ; Thomas Recording, Giessen, Germany). Wideband signals were acquired continuously via an op-amp-based headstage amplifier (HST/8o50-G1-GR, 1x gain, Plexon Inc., Dallas, TX, USA), passed through a preamplifier (PBX2/16wb, 1000x gain; Plexon Inc., Dallas, TX, USA) and digitized at 40 kHz. For spike sorting the raw signal was band-pass filtered 300–3000 Hz and spikes were sorted using the Matlab-based Wave_clus software to yield single-unit spike trains (Quiroga et al., 2004). Wave_clus performs unsupervised spike detection and sorting using wavelets and super-paramagnetic clustering. All automatic detection thresholds and sorting solutions were examined individually and adjusted if needed. Field potentials were low-pass filtered using a 200-Hz cut-off Butterworth filter and downsampled offline to 5000 Hz. Power spectral densities of the signals recorded from PFC were estimated by means of Welch periodograms (window length: 2 s, overlap: 90%, Hanning window, resolution 1 Hz/bin). Treatment effects in the energy values (mean power of all frequencies within the band) for the delta (0.5–3.5 Hz), theta (4–7.5 Hz), alpha (8–12 Hz), mu (10–12 Hz), beta (13–30 Hz) and gamma (30–70 Hz) frequency bands were assessed using the Mann–Whitney U test for equal medians. To investigate drug effects on spike-local field potential (LFP) synchrony, the LFP signal was band-pass filtered using a 3-Hz moving window with a second-order Butterworth filter. A Hilbert transform was applied to obtain the instantaneous phase for each frequency range (Fig. 3A, B) (Saleem et al., 2010). Average phase angle for each spike at each frequency window was calculated using the Matlab toolbox CircStat (Berens, 2009). Further analyses were calculated using Neuroexplorer (Nex Technologies, Littleton, MA, USA) and custom-written Matlab routines.


Disruption of medial prefrontal synchrony in the subchronic phencyclidine model of schizophrenia in rats.

Young AM, Stubbendorff C, Valencia M, Gerdjikov TV - Neuroscience (2014)

mPFC putative pyramidal neurons show enhanced synchrony with lower LFP frequencies in PCP-treated animals. (A) Example of single-unit activity in a neuron recorded from a PCP-treated animal (top) juxtaposed to ongoing LFP oscillations (middle) recorded from the same electrode and filtered at 1–5 Hz (green trace). The instantaneous phase (bottom) of the 1–5-Hz oscillations is obtained using a Hilbert transformation. It was used to calculate resultant vector length for spike times relative to the phases of the 1–5-Hz frequency band as shown in the polar plot on the right. Resultant vector length reflects the magnitude of the directionality effect and thus represents the degree of locking. (B) Example of single-unit activity juxtaposed to ongoing LFP oscillations in a neuron recorded from a saline-treated animal; data are represented as in (A). (C) Population locking of single units across frequencies for PCP and saline-treated animals. Neurons recorded in PCP-treated animals showed enhanced locking to lower frequencies. Error bands are calculated across neurons and represent SEM. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
© Copyright Policy - CC BY
Related In: Results  -  Collection

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Show All Figures
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f0015: mPFC putative pyramidal neurons show enhanced synchrony with lower LFP frequencies in PCP-treated animals. (A) Example of single-unit activity in a neuron recorded from a PCP-treated animal (top) juxtaposed to ongoing LFP oscillations (middle) recorded from the same electrode and filtered at 1–5 Hz (green trace). The instantaneous phase (bottom) of the 1–5-Hz oscillations is obtained using a Hilbert transformation. It was used to calculate resultant vector length for spike times relative to the phases of the 1–5-Hz frequency band as shown in the polar plot on the right. Resultant vector length reflects the magnitude of the directionality effect and thus represents the degree of locking. (B) Example of single-unit activity juxtaposed to ongoing LFP oscillations in a neuron recorded from a saline-treated animal; data are represented as in (A). (C) Population locking of single units across frequencies for PCP and saline-treated animals. Neurons recorded in PCP-treated animals showed enhanced locking to lower frequencies. Error bands are calculated across neurons and represent SEM. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
Mentions: mPFC was targeted with a left-side craniotomy with coordinates: +3.2 mm AP; 0.5 mm ML; −2 mm DV (Paxinos and Watson, 2007). Recording electrodes consisted of quartz glass-coated platinum/tungsten wires pulled and ground to custom shapes in our laboratory (shank diameter 80 μm; diameter of the metal core 23 μm; free tip length ∼8 μm; impedance, 1–3 MΩ; Thomas Recording, Giessen, Germany). Wideband signals were acquired continuously via an op-amp-based headstage amplifier (HST/8o50-G1-GR, 1x gain, Plexon Inc., Dallas, TX, USA), passed through a preamplifier (PBX2/16wb, 1000x gain; Plexon Inc., Dallas, TX, USA) and digitized at 40 kHz. For spike sorting the raw signal was band-pass filtered 300–3000 Hz and spikes were sorted using the Matlab-based Wave_clus software to yield single-unit spike trains (Quiroga et al., 2004). Wave_clus performs unsupervised spike detection and sorting using wavelets and super-paramagnetic clustering. All automatic detection thresholds and sorting solutions were examined individually and adjusted if needed. Field potentials were low-pass filtered using a 200-Hz cut-off Butterworth filter and downsampled offline to 5000 Hz. Power spectral densities of the signals recorded from PFC were estimated by means of Welch periodograms (window length: 2 s, overlap: 90%, Hanning window, resolution 1 Hz/bin). Treatment effects in the energy values (mean power of all frequencies within the band) for the delta (0.5–3.5 Hz), theta (4–7.5 Hz), alpha (8–12 Hz), mu (10–12 Hz), beta (13–30 Hz) and gamma (30–70 Hz) frequency bands were assessed using the Mann–Whitney U test for equal medians. To investigate drug effects on spike-local field potential (LFP) synchrony, the LFP signal was band-pass filtered using a 3-Hz moving window with a second-order Butterworth filter. A Hilbert transform was applied to obtain the instantaneous phase for each frequency range (Fig. 3A, B) (Saleem et al., 2010). Average phase angle for each spike at each frequency window was calculated using the Matlab toolbox CircStat (Berens, 2009). Further analyses were calculated using Neuroexplorer (Nex Technologies, Littleton, MA, USA) and custom-written Matlab routines.

Bottom Line: We found that this regimen reduced theta oscillations in the medial prefrontal cortex.It further produced abnormal cortical synchronization in putative cortical pyramidal cells.These alterations in prefrontal cortex functioning may contribute to cognitive deficits seen in subchronic NMDA antagonist pre-treated animals in prefrontal-dependent tasks.

View Article: PubMed Central - PubMed

Affiliation: School of Psychology, College of Medicine, Biological Sciences and Psychology, University of Leicester, United Kingdom.

Show MeSH
Related in: MedlinePlus