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Controlling RECK miR21 Promotes Tumor Cell Invasion and Is Related to Biochemical Recurrence in Prostate Cancer.

Leite KR, Reis ST, Viana N, Morais DR, Moura CM, Silva IA, Pontes J, Katz B, Srougi M - J Cancer (2015)

Bottom Line: We defined different as an expression level greater or less than 1.1 with p<0.05.Transfection of miR-21 using lipid based assay in DU145 cell line, showed decrease in expression of RECK resulting in increase in expression of MMP9.We conclude that miR-21 overexpression is related to increased biochemical recurrence after surgical treatment of prostate cancer.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Medical Research, LIM55, Urology Department, University of Sao Paulo Medical School, Sao Paulo, Brazil.

ABSTRACT
The search for biomarkers to characterize prostate cancer aggressiveness has been the objective for the majority of researchers involved with the most prevalent tumor in men. MiRNAs are important for the control of many cellular functions and their deregulation is involved with tumor development and progression. To find miRNAs differentially expressed in prostate cancer and their relation to prognostic factors and biochemical recurrence we studied 53 surgical specimens from men who underwent radical prostatectomy, through a microarray analysis using the microarray platform (GeneChip® miRNA Array - Affymetrix) with more than 46,000 probes and 847 mature human miRNAs and transcripts. We defined different as an expression level greater or less than 1.1 with p<0.05. The validation study using qRT-PCR had confirmed miR21 as overexpressed in tumor that have recurred with a risk of 2.5. Transfection of miR-21 using lipid based assay in DU145 cell line, showed decrease in expression of RECK resulting in increase in expression of MMP9. Invasion assay with Matrigel showed increase in tumor cell invasion after miR-21 transfection. We conclude that miR-21 overexpression is related to increased biochemical recurrence after surgical treatment of prostate cancer. And the negative control of RECK results in overexpression of MMP9 promotes increasing tumor cell invasion supporting miR-21 as an oncomiR related to aggressiveness in prostate cancer.

No MeSH data available.


Related in: MedlinePlus

miRNAs simultaneously under or overexpressed considering different unfavorable prognostic factors and biochemical recurrence after radical prostatectomy.
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Figure 1: miRNAs simultaneously under or overexpressed considering different unfavorable prognostic factors and biochemical recurrence after radical prostatectomy.

Mentions: When all results were compared, there were few miRNAs commonly under or overexpressed by different unfavorable prognostic factors and related to biochemical recurrence (Figure 1). miRNAs 92, 450 and 16 were downexpressed by tumors that have recurred and were non organ-confined or when the pre-operatory PSA was >10ng/mL. Whereas miRNAs 885, 1226 and 1202 were overexpressed by tumors that have recurred and were non organ-confined or the pre-operatory PSA was >10ng/mL. The validation by qRT-PCR of these miRNAs in the same group of patients previously studied using the microarray did not confirm a relationship between their expression, prognostic factors or biochemical recurrence.


Controlling RECK miR21 Promotes Tumor Cell Invasion and Is Related to Biochemical Recurrence in Prostate Cancer.

Leite KR, Reis ST, Viana N, Morais DR, Moura CM, Silva IA, Pontes J, Katz B, Srougi M - J Cancer (2015)

miRNAs simultaneously under or overexpressed considering different unfavorable prognostic factors and biochemical recurrence after radical prostatectomy.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4317766&req=5

Figure 1: miRNAs simultaneously under or overexpressed considering different unfavorable prognostic factors and biochemical recurrence after radical prostatectomy.
Mentions: When all results were compared, there were few miRNAs commonly under or overexpressed by different unfavorable prognostic factors and related to biochemical recurrence (Figure 1). miRNAs 92, 450 and 16 were downexpressed by tumors that have recurred and were non organ-confined or when the pre-operatory PSA was >10ng/mL. Whereas miRNAs 885, 1226 and 1202 were overexpressed by tumors that have recurred and were non organ-confined or the pre-operatory PSA was >10ng/mL. The validation by qRT-PCR of these miRNAs in the same group of patients previously studied using the microarray did not confirm a relationship between their expression, prognostic factors or biochemical recurrence.

Bottom Line: We defined different as an expression level greater or less than 1.1 with p<0.05.Transfection of miR-21 using lipid based assay in DU145 cell line, showed decrease in expression of RECK resulting in increase in expression of MMP9.We conclude that miR-21 overexpression is related to increased biochemical recurrence after surgical treatment of prostate cancer.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Medical Research, LIM55, Urology Department, University of Sao Paulo Medical School, Sao Paulo, Brazil.

ABSTRACT
The search for biomarkers to characterize prostate cancer aggressiveness has been the objective for the majority of researchers involved with the most prevalent tumor in men. MiRNAs are important for the control of many cellular functions and their deregulation is involved with tumor development and progression. To find miRNAs differentially expressed in prostate cancer and their relation to prognostic factors and biochemical recurrence we studied 53 surgical specimens from men who underwent radical prostatectomy, through a microarray analysis using the microarray platform (GeneChip® miRNA Array - Affymetrix) with more than 46,000 probes and 847 mature human miRNAs and transcripts. We defined different as an expression level greater or less than 1.1 with p<0.05. The validation study using qRT-PCR had confirmed miR21 as overexpressed in tumor that have recurred with a risk of 2.5. Transfection of miR-21 using lipid based assay in DU145 cell line, showed decrease in expression of RECK resulting in increase in expression of MMP9. Invasion assay with Matrigel showed increase in tumor cell invasion after miR-21 transfection. We conclude that miR-21 overexpression is related to increased biochemical recurrence after surgical treatment of prostate cancer. And the negative control of RECK results in overexpression of MMP9 promotes increasing tumor cell invasion supporting miR-21 as an oncomiR related to aggressiveness in prostate cancer.

No MeSH data available.


Related in: MedlinePlus