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De novo assembly, transcriptome characterization, lignin accumulation, and anatomic characteristics: novel insights into lignin biosynthesis during celery leaf development.

Jia XL, Wang GL, Xiong F, Yu XR, Xu ZS, Wang F, Xiong AS - Sci Rep (2015)

Bottom Line: Regulating lignin synthesis in celery growth development has a significant economic value.Lignin accumulation in different tissues and at different stages of celery development coincides with the anatomic characteristics and transcript levels of genes involved in lignin biosynthesis.Identifying the genes that encode lignin biosynthesis-related enzymes accompanied by lignin distribution may help elucidate the regulatory mechanisms of lignin biosynthesis in celery.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, China.

ABSTRACT
Celery of the family Apiaceae is a biennial herb that is cultivated and consumed worldwide. Lignin is essential for cell wall structural integrity, stem strength, water transport, mechanical support, and plant pathogen defense. This study discussed the mechanism of lignin formation at different stages of celery development. The transcriptome profile, lignin distribution, anatomical characteristics, and expression profile of leaves at three stages were analyzed. Regulating lignin synthesis in celery growth development has a significant economic value. Celery leaves at three stages were collected, and Illumina paired-end sequencing technology was used to analyze large-scale transcriptome sequences. From Stage 1 to 3, the collenchyma and vascular bundles in the petioles and leaf blades thickened and expanded, whereas the phloem and the xylem extensively developed. Spongy and palisade mesophyll tissues further developed and were tightly arranged. Lignin accumulation increased in the petioles and the mesophyll (palisade and spongy), and the xylem showed strong lignification. Lignin accumulation in different tissues and at different stages of celery development coincides with the anatomic characteristics and transcript levels of genes involved in lignin biosynthesis. Identifying the genes that encode lignin biosynthesis-related enzymes accompanied by lignin distribution may help elucidate the regulatory mechanisms of lignin biosynthesis in celery.

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Related in: MedlinePlus

Scanning electron micrographs of ‘Ventura' petiole at Stage 3.(A): Structure of petiole transverse sections; (B): structure of vascular bundle; (C): structure of collenchyma; (D): structure of cells.
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f7: Scanning electron micrographs of ‘Ventura' petiole at Stage 3.(A): Structure of petiole transverse sections; (B): structure of vascular bundle; (C): structure of collenchyma; (D): structure of cells.

Mentions: Petiole transection structure was characterized with SEM (Figure 7).The vascular bundles in petioles serve as important channels for material transport, and collenchyma strengthen and support tissues in plants2223. The collenchyma and vascular bundles in the petioles were thick and large, and the cells were large and tightly arranged (Figure 7A–D).


De novo assembly, transcriptome characterization, lignin accumulation, and anatomic characteristics: novel insights into lignin biosynthesis during celery leaf development.

Jia XL, Wang GL, Xiong F, Yu XR, Xu ZS, Wang F, Xiong AS - Sci Rep (2015)

Scanning electron micrographs of ‘Ventura' petiole at Stage 3.(A): Structure of petiole transverse sections; (B): structure of vascular bundle; (C): structure of collenchyma; (D): structure of cells.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4317703&req=5

f7: Scanning electron micrographs of ‘Ventura' petiole at Stage 3.(A): Structure of petiole transverse sections; (B): structure of vascular bundle; (C): structure of collenchyma; (D): structure of cells.
Mentions: Petiole transection structure was characterized with SEM (Figure 7).The vascular bundles in petioles serve as important channels for material transport, and collenchyma strengthen and support tissues in plants2223. The collenchyma and vascular bundles in the petioles were thick and large, and the cells were large and tightly arranged (Figure 7A–D).

Bottom Line: Regulating lignin synthesis in celery growth development has a significant economic value.Lignin accumulation in different tissues and at different stages of celery development coincides with the anatomic characteristics and transcript levels of genes involved in lignin biosynthesis.Identifying the genes that encode lignin biosynthesis-related enzymes accompanied by lignin distribution may help elucidate the regulatory mechanisms of lignin biosynthesis in celery.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, China.

ABSTRACT
Celery of the family Apiaceae is a biennial herb that is cultivated and consumed worldwide. Lignin is essential for cell wall structural integrity, stem strength, water transport, mechanical support, and plant pathogen defense. This study discussed the mechanism of lignin formation at different stages of celery development. The transcriptome profile, lignin distribution, anatomical characteristics, and expression profile of leaves at three stages were analyzed. Regulating lignin synthesis in celery growth development has a significant economic value. Celery leaves at three stages were collected, and Illumina paired-end sequencing technology was used to analyze large-scale transcriptome sequences. From Stage 1 to 3, the collenchyma and vascular bundles in the petioles and leaf blades thickened and expanded, whereas the phloem and the xylem extensively developed. Spongy and palisade mesophyll tissues further developed and were tightly arranged. Lignin accumulation increased in the petioles and the mesophyll (palisade and spongy), and the xylem showed strong lignification. Lignin accumulation in different tissues and at different stages of celery development coincides with the anatomic characteristics and transcript levels of genes involved in lignin biosynthesis. Identifying the genes that encode lignin biosynthesis-related enzymes accompanied by lignin distribution may help elucidate the regulatory mechanisms of lignin biosynthesis in celery.

Show MeSH
Related in: MedlinePlus