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De novo assembly, transcriptome characterization, lignin accumulation, and anatomic characteristics: novel insights into lignin biosynthesis during celery leaf development.

Jia XL, Wang GL, Xiong F, Yu XR, Xu ZS, Wang F, Xiong AS - Sci Rep (2015)

Bottom Line: Regulating lignin synthesis in celery growth development has a significant economic value.Lignin accumulation in different tissues and at different stages of celery development coincides with the anatomic characteristics and transcript levels of genes involved in lignin biosynthesis.Identifying the genes that encode lignin biosynthesis-related enzymes accompanied by lignin distribution may help elucidate the regulatory mechanisms of lignin biosynthesis in celery.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, China.

ABSTRACT
Celery of the family Apiaceae is a biennial herb that is cultivated and consumed worldwide. Lignin is essential for cell wall structural integrity, stem strength, water transport, mechanical support, and plant pathogen defense. This study discussed the mechanism of lignin formation at different stages of celery development. The transcriptome profile, lignin distribution, anatomical characteristics, and expression profile of leaves at three stages were analyzed. Regulating lignin synthesis in celery growth development has a significant economic value. Celery leaves at three stages were collected, and Illumina paired-end sequencing technology was used to analyze large-scale transcriptome sequences. From Stage 1 to 3, the collenchyma and vascular bundles in the petioles and leaf blades thickened and expanded, whereas the phloem and the xylem extensively developed. Spongy and palisade mesophyll tissues further developed and were tightly arranged. Lignin accumulation increased in the petioles and the mesophyll (palisade and spongy), and the xylem showed strong lignification. Lignin accumulation in different tissues and at different stages of celery development coincides with the anatomic characteristics and transcript levels of genes involved in lignin biosynthesis. Identifying the genes that encode lignin biosynthesis-related enzymes accompanied by lignin distribution may help elucidate the regulatory mechanisms of lignin biosynthesis in celery.

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Related in: MedlinePlus

Structural comparison of ‘Ventura' petiole.(A), (B): Stage 1 of ‘Ventura' × 200; (C), (D): Stage 2 of ‘Ventura' × 200; (E), (F): Stage 3 of ‘Ventura' × 200. Ep: epidermis; C: collenchyma; P: phloem; X: xylem.
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f6: Structural comparison of ‘Ventura' petiole.(A), (B): Stage 1 of ‘Ventura' × 200; (C), (D): Stage 2 of ‘Ventura' × 200; (E), (F): Stage 3 of ‘Ventura' × 200. Ep: epidermis; C: collenchyma; P: phloem; X: xylem.

Mentions: This study comprehensively investigated the structural leaf development of ‘Ventura' using resin-embedding microtomy and scanning electron microscopy (SEM). As shown in Figure 5, the leaf blade gradually thickened, and the spongy and palisade mesophyll tissues were tightly arranged at the three stages. The collenchyma and vascular bundles in the leaf vein thickened and expanded during leaf growth and development. As shown in Figure 6, the collenchyma grew, the vascular bundles further expanded, the phloem and xylem extensively developed, and the epidermal cells of the petioles expanded from Stage 1 to Stage 3.


De novo assembly, transcriptome characterization, lignin accumulation, and anatomic characteristics: novel insights into lignin biosynthesis during celery leaf development.

Jia XL, Wang GL, Xiong F, Yu XR, Xu ZS, Wang F, Xiong AS - Sci Rep (2015)

Structural comparison of ‘Ventura' petiole.(A), (B): Stage 1 of ‘Ventura' × 200; (C), (D): Stage 2 of ‘Ventura' × 200; (E), (F): Stage 3 of ‘Ventura' × 200. Ep: epidermis; C: collenchyma; P: phloem; X: xylem.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4317703&req=5

f6: Structural comparison of ‘Ventura' petiole.(A), (B): Stage 1 of ‘Ventura' × 200; (C), (D): Stage 2 of ‘Ventura' × 200; (E), (F): Stage 3 of ‘Ventura' × 200. Ep: epidermis; C: collenchyma; P: phloem; X: xylem.
Mentions: This study comprehensively investigated the structural leaf development of ‘Ventura' using resin-embedding microtomy and scanning electron microscopy (SEM). As shown in Figure 5, the leaf blade gradually thickened, and the spongy and palisade mesophyll tissues were tightly arranged at the three stages. The collenchyma and vascular bundles in the leaf vein thickened and expanded during leaf growth and development. As shown in Figure 6, the collenchyma grew, the vascular bundles further expanded, the phloem and xylem extensively developed, and the epidermal cells of the petioles expanded from Stage 1 to Stage 3.

Bottom Line: Regulating lignin synthesis in celery growth development has a significant economic value.Lignin accumulation in different tissues and at different stages of celery development coincides with the anatomic characteristics and transcript levels of genes involved in lignin biosynthesis.Identifying the genes that encode lignin biosynthesis-related enzymes accompanied by lignin distribution may help elucidate the regulatory mechanisms of lignin biosynthesis in celery.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, China.

ABSTRACT
Celery of the family Apiaceae is a biennial herb that is cultivated and consumed worldwide. Lignin is essential for cell wall structural integrity, stem strength, water transport, mechanical support, and plant pathogen defense. This study discussed the mechanism of lignin formation at different stages of celery development. The transcriptome profile, lignin distribution, anatomical characteristics, and expression profile of leaves at three stages were analyzed. Regulating lignin synthesis in celery growth development has a significant economic value. Celery leaves at three stages were collected, and Illumina paired-end sequencing technology was used to analyze large-scale transcriptome sequences. From Stage 1 to 3, the collenchyma and vascular bundles in the petioles and leaf blades thickened and expanded, whereas the phloem and the xylem extensively developed. Spongy and palisade mesophyll tissues further developed and were tightly arranged. Lignin accumulation increased in the petioles and the mesophyll (palisade and spongy), and the xylem showed strong lignification. Lignin accumulation in different tissues and at different stages of celery development coincides with the anatomic characteristics and transcript levels of genes involved in lignin biosynthesis. Identifying the genes that encode lignin biosynthesis-related enzymes accompanied by lignin distribution may help elucidate the regulatory mechanisms of lignin biosynthesis in celery.

Show MeSH
Related in: MedlinePlus