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De novo assembly, transcriptome characterization, lignin accumulation, and anatomic characteristics: novel insights into lignin biosynthesis during celery leaf development.

Jia XL, Wang GL, Xiong F, Yu XR, Xu ZS, Wang F, Xiong AS - Sci Rep (2015)

Bottom Line: Regulating lignin synthesis in celery growth development has a significant economic value.Lignin accumulation in different tissues and at different stages of celery development coincides with the anatomic characteristics and transcript levels of genes involved in lignin biosynthesis.Identifying the genes that encode lignin biosynthesis-related enzymes accompanied by lignin distribution may help elucidate the regulatory mechanisms of lignin biosynthesis in celery.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, China.

ABSTRACT
Celery of the family Apiaceae is a biennial herb that is cultivated and consumed worldwide. Lignin is essential for cell wall structural integrity, stem strength, water transport, mechanical support, and plant pathogen defense. This study discussed the mechanism of lignin formation at different stages of celery development. The transcriptome profile, lignin distribution, anatomical characteristics, and expression profile of leaves at three stages were analyzed. Regulating lignin synthesis in celery growth development has a significant economic value. Celery leaves at three stages were collected, and Illumina paired-end sequencing technology was used to analyze large-scale transcriptome sequences. From Stage 1 to 3, the collenchyma and vascular bundles in the petioles and leaf blades thickened and expanded, whereas the phloem and the xylem extensively developed. Spongy and palisade mesophyll tissues further developed and were tightly arranged. Lignin accumulation increased in the petioles and the mesophyll (palisade and spongy), and the xylem showed strong lignification. Lignin accumulation in different tissues and at different stages of celery development coincides with the anatomic characteristics and transcript levels of genes involved in lignin biosynthesis. Identifying the genes that encode lignin biosynthesis-related enzymes accompanied by lignin distribution may help elucidate the regulatory mechanisms of lignin biosynthesis in celery.

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Expression profiles of genes involved in lignin biosynthesis at different stages of ‘Ventura'.Each bar represents the mean value results from triplicate experiments ± SD.
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f13: Expression profiles of genes involved in lignin biosynthesis at different stages of ‘Ventura'.Each bar represents the mean value results from triplicate experiments ± SD.

Mentions: The relative transcript level of AgCOMT in the petioles increased and peaked at Stage 1, decreased at Stage 2, and then continued to decrease and diminish at Stage 3. The transcript levels of AgC3H, Ag4CL, and AgPOD gradually increased among the three stages, with no significant difference in the relative expression of AgC3H and AgPOD between the two former stages. The relative expression levels of AgPAL, AgC4H, AgF5H, AgHCT, AgCCoAOMT, AgCCR, and AgCAD increased at Stage 2 and then decreased at Stage 3. The transcript levels of AgPAL, AgC4H, and AgCCoAOMT were higher at Stage 3 than at Stage 1, but that of AgHCT was lower at Stage 3 than at Stage 1. No significant difference in the transcript levels of AgF5H, AgCCR, and AgCAD was detected between Stage 1 and Stage 3 (Figure 13).


De novo assembly, transcriptome characterization, lignin accumulation, and anatomic characteristics: novel insights into lignin biosynthesis during celery leaf development.

Jia XL, Wang GL, Xiong F, Yu XR, Xu ZS, Wang F, Xiong AS - Sci Rep (2015)

Expression profiles of genes involved in lignin biosynthesis at different stages of ‘Ventura'.Each bar represents the mean value results from triplicate experiments ± SD.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4317703&req=5

f13: Expression profiles of genes involved in lignin biosynthesis at different stages of ‘Ventura'.Each bar represents the mean value results from triplicate experiments ± SD.
Mentions: The relative transcript level of AgCOMT in the petioles increased and peaked at Stage 1, decreased at Stage 2, and then continued to decrease and diminish at Stage 3. The transcript levels of AgC3H, Ag4CL, and AgPOD gradually increased among the three stages, with no significant difference in the relative expression of AgC3H and AgPOD between the two former stages. The relative expression levels of AgPAL, AgC4H, AgF5H, AgHCT, AgCCoAOMT, AgCCR, and AgCAD increased at Stage 2 and then decreased at Stage 3. The transcript levels of AgPAL, AgC4H, and AgCCoAOMT were higher at Stage 3 than at Stage 1, but that of AgHCT was lower at Stage 3 than at Stage 1. No significant difference in the transcript levels of AgF5H, AgCCR, and AgCAD was detected between Stage 1 and Stage 3 (Figure 13).

Bottom Line: Regulating lignin synthesis in celery growth development has a significant economic value.Lignin accumulation in different tissues and at different stages of celery development coincides with the anatomic characteristics and transcript levels of genes involved in lignin biosynthesis.Identifying the genes that encode lignin biosynthesis-related enzymes accompanied by lignin distribution may help elucidate the regulatory mechanisms of lignin biosynthesis in celery.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, China.

ABSTRACT
Celery of the family Apiaceae is a biennial herb that is cultivated and consumed worldwide. Lignin is essential for cell wall structural integrity, stem strength, water transport, mechanical support, and plant pathogen defense. This study discussed the mechanism of lignin formation at different stages of celery development. The transcriptome profile, lignin distribution, anatomical characteristics, and expression profile of leaves at three stages were analyzed. Regulating lignin synthesis in celery growth development has a significant economic value. Celery leaves at three stages were collected, and Illumina paired-end sequencing technology was used to analyze large-scale transcriptome sequences. From Stage 1 to 3, the collenchyma and vascular bundles in the petioles and leaf blades thickened and expanded, whereas the phloem and the xylem extensively developed. Spongy and palisade mesophyll tissues further developed and were tightly arranged. Lignin accumulation increased in the petioles and the mesophyll (palisade and spongy), and the xylem showed strong lignification. Lignin accumulation in different tissues and at different stages of celery development coincides with the anatomic characteristics and transcript levels of genes involved in lignin biosynthesis. Identifying the genes that encode lignin biosynthesis-related enzymes accompanied by lignin distribution may help elucidate the regulatory mechanisms of lignin biosynthesis in celery.

Show MeSH
Related in: MedlinePlus