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De novo assembly, transcriptome characterization, lignin accumulation, and anatomic characteristics: novel insights into lignin biosynthesis during celery leaf development.

Jia XL, Wang GL, Xiong F, Yu XR, Xu ZS, Wang F, Xiong AS - Sci Rep (2015)

Bottom Line: Regulating lignin synthesis in celery growth development has a significant economic value.Lignin accumulation in different tissues and at different stages of celery development coincides with the anatomic characteristics and transcript levels of genes involved in lignin biosynthesis.Identifying the genes that encode lignin biosynthesis-related enzymes accompanied by lignin distribution may help elucidate the regulatory mechanisms of lignin biosynthesis in celery.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, China.

ABSTRACT
Celery of the family Apiaceae is a biennial herb that is cultivated and consumed worldwide. Lignin is essential for cell wall structural integrity, stem strength, water transport, mechanical support, and plant pathogen defense. This study discussed the mechanism of lignin formation at different stages of celery development. The transcriptome profile, lignin distribution, anatomical characteristics, and expression profile of leaves at three stages were analyzed. Regulating lignin synthesis in celery growth development has a significant economic value. Celery leaves at three stages were collected, and Illumina paired-end sequencing technology was used to analyze large-scale transcriptome sequences. From Stage 1 to 3, the collenchyma and vascular bundles in the petioles and leaf blades thickened and expanded, whereas the phloem and the xylem extensively developed. Spongy and palisade mesophyll tissues further developed and were tightly arranged. Lignin accumulation increased in the petioles and the mesophyll (palisade and spongy), and the xylem showed strong lignification. Lignin accumulation in different tissues and at different stages of celery development coincides with the anatomic characteristics and transcript levels of genes involved in lignin biosynthesis. Identifying the genes that encode lignin biosynthesis-related enzymes accompanied by lignin distribution may help elucidate the regulatory mechanisms of lignin biosynthesis in celery.

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Related in: MedlinePlus

Expression profiles of genes involved in lignin biosynthesis in different tissues of ‘Ventura'.Each bar represents the mean value results from triplicate experiments ± SD.
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f12: Expression profiles of genes involved in lignin biosynthesis in different tissues of ‘Ventura'.Each bar represents the mean value results from triplicate experiments ± SD.

Mentions: The 11 genes were differentially expressed in the roots, stems, petioles, and leaf blades of ‘Ventura' (Figure 12). These genes may perform different functions in different celery tissues and thus affect the growth and development of celery plants. The relative expression level of AgCCoAOMT was higher in the roots than in the other three tissues, and no significant difference in its expression was detected among the stems, petioles, and leaf blades. The transcript levels of AgCOMT were the highest in the stems, followed by the petioles and leaf blades, and then the roots. The transcript levels of AgC4H, AgF5H, and AgHCT were higher in the petioles than in the other three tissues. The relative expression levels of AgC4H and AgHCT were slightly lower in the roots and leaf blades and remained low in the stems. The expression level of AgF5H was lower in the roots and stems than in the leaf blades and petioles. The transcript levels of AgPAL, AgC3H, Ag4CL, AgCCR, AgCAD, and AgPOD were higher in the leaf blades than in the other three tissues. The relative expression levels of AgPAL, AgC3H, AgCCR, and AgCAD were lower in the roots, stems, and petioles than in the leaf blades, with no significant difference among the three former tissues. The transcript level of Ag4CL was slightly low in the stems and remained relatively low in the roots and petioles, with no significant difference between the two latter tissues. The relative expression of AgPOD was relatively low in the roots and petioles with no significant difference, and the lowest expression level was observed in the stems (Figure 12).


De novo assembly, transcriptome characterization, lignin accumulation, and anatomic characteristics: novel insights into lignin biosynthesis during celery leaf development.

Jia XL, Wang GL, Xiong F, Yu XR, Xu ZS, Wang F, Xiong AS - Sci Rep (2015)

Expression profiles of genes involved in lignin biosynthesis in different tissues of ‘Ventura'.Each bar represents the mean value results from triplicate experiments ± SD.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4317703&req=5

f12: Expression profiles of genes involved in lignin biosynthesis in different tissues of ‘Ventura'.Each bar represents the mean value results from triplicate experiments ± SD.
Mentions: The 11 genes were differentially expressed in the roots, stems, petioles, and leaf blades of ‘Ventura' (Figure 12). These genes may perform different functions in different celery tissues and thus affect the growth and development of celery plants. The relative expression level of AgCCoAOMT was higher in the roots than in the other three tissues, and no significant difference in its expression was detected among the stems, petioles, and leaf blades. The transcript levels of AgCOMT were the highest in the stems, followed by the petioles and leaf blades, and then the roots. The transcript levels of AgC4H, AgF5H, and AgHCT were higher in the petioles than in the other three tissues. The relative expression levels of AgC4H and AgHCT were slightly lower in the roots and leaf blades and remained low in the stems. The expression level of AgF5H was lower in the roots and stems than in the leaf blades and petioles. The transcript levels of AgPAL, AgC3H, Ag4CL, AgCCR, AgCAD, and AgPOD were higher in the leaf blades than in the other three tissues. The relative expression levels of AgPAL, AgC3H, AgCCR, and AgCAD were lower in the roots, stems, and petioles than in the leaf blades, with no significant difference among the three former tissues. The transcript level of Ag4CL was slightly low in the stems and remained relatively low in the roots and petioles, with no significant difference between the two latter tissues. The relative expression of AgPOD was relatively low in the roots and petioles with no significant difference, and the lowest expression level was observed in the stems (Figure 12).

Bottom Line: Regulating lignin synthesis in celery growth development has a significant economic value.Lignin accumulation in different tissues and at different stages of celery development coincides with the anatomic characteristics and transcript levels of genes involved in lignin biosynthesis.Identifying the genes that encode lignin biosynthesis-related enzymes accompanied by lignin distribution may help elucidate the regulatory mechanisms of lignin biosynthesis in celery.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, China.

ABSTRACT
Celery of the family Apiaceae is a biennial herb that is cultivated and consumed worldwide. Lignin is essential for cell wall structural integrity, stem strength, water transport, mechanical support, and plant pathogen defense. This study discussed the mechanism of lignin formation at different stages of celery development. The transcriptome profile, lignin distribution, anatomical characteristics, and expression profile of leaves at three stages were analyzed. Regulating lignin synthesis in celery growth development has a significant economic value. Celery leaves at three stages were collected, and Illumina paired-end sequencing technology was used to analyze large-scale transcriptome sequences. From Stage 1 to 3, the collenchyma and vascular bundles in the petioles and leaf blades thickened and expanded, whereas the phloem and the xylem extensively developed. Spongy and palisade mesophyll tissues further developed and were tightly arranged. Lignin accumulation increased in the petioles and the mesophyll (palisade and spongy), and the xylem showed strong lignification. Lignin accumulation in different tissues and at different stages of celery development coincides with the anatomic characteristics and transcript levels of genes involved in lignin biosynthesis. Identifying the genes that encode lignin biosynthesis-related enzymes accompanied by lignin distribution may help elucidate the regulatory mechanisms of lignin biosynthesis in celery.

Show MeSH
Related in: MedlinePlus